Direct counts of cells in liquid samples can be performed using a Petroff-Hausser counting chamber. Research this method and describe how it compares to the viable plate count method of determining the number of CFU in a sample.

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Answer 1

The viable plate count is the most commonly used method to estimate the number of viable cells in a sample; while the Petroff-Hausser counting chamber is a direct microscopic count method generally used for counting bacteria and sperm cells.

The Petroff-Hausser counter is a counting chamber used to count microorganisms and cells (e.g., bacteria and spermatic cells) under the microscope.

The Petroff-Hausser counter chamber is divided into 25 large squares which are in turn divided by double lines. Moreover, within each large square, there also are 16 small squares divided by single lines.

Petroff-Hausser counting chambers are often used to directly determine the number of bacteria in a culture or liquid medium. Petroff-Hausser counting chambers are used to count the number of cells in a given volume of culture liquid by observing 10 to 20 microscope fields.

The viable plate count, also known as simply plate count, is the most commonly used procedure to estimate the number of viable cells in a sample.

The viable plate count method consists in obtaining serial dilutions of a sample having viable microorganisms/cells which are plated onto a growth medium.

This method (viable plate count) can be used to determine the number of actively dividing cells.

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Related Questions

What will the percentage of cytosine be in this segment of DNA?

Answers

Answer:

15%

Together, adenine and thymine compose 70% of the segment. This means that 30% of the section is composed of guanine-cytosine pairs. Since these two bases will be equal in quantity, 15% of the DNA section will be cytosine bases.

What do MSDS sheets do, in your own words

Answers

Answer: The MSDS lists the hazardous ingredients of a product, its physical and chemical characteristics (e.g. flammability, explosive properties), its effect on human health, the chemicals with which it can adversely react, handling precautions, the types of measures that can be used to control exposure

Explanation:

A large section of tropical rainforest is cleared to build roads for mining. Select the least likely outcome of this habitat fragmentation from the options below. a.) Species that require open grazing areas may not survive. b.) Species will quickly adapt and re-populate the area. c.) The environment on the habitat edges will support different plants and animals. d.) Subpopulations of species may emerge.

Answers

Answer:

B

Explanation:

B - Species, especially ones with small niches, that looses their niches will likely die out or move to another location. Roads Will, in general, also disrupt the ecosystem (or the remains of it) and its inhabitants, which might lead to migration once again. That would be my reasoning at least. (:

Fertilization, Fruit and Seed Formation x3 3. a. name the two processes that lead to seed formation in flowering

Answers

Answer:

Pollination, the transfer of pollen from flower-to-flower in angiosperms or cone ... In angiosperms, the process of seed development begins with double ...

Missing: x3 ‎| Must include: x3

For every 100ml of deoxygenated blood delivers approximately _____ml of CO2 to the alveoli.​

Answers

Answer:

For every 100ml of deoxygenated blood delivers approximately __4___ml of CO2 to the alveoli.

Answer:

4ml

Explanation:

For every 100ml of deoxygenated blood delivers approximately 4 ml of CO2 to the alveoli.

Hope it is helpful....


What component of Earth's atmosphere exists entirely as a result of photosynthesis?
oxygen pas
n mas
O water vapor
O nitrogen gas
O carbon dioxide gas

Answers

Answer:

Explanation:

Carbon dioxide

The last one is correct
Hope its help for your questions

Two species have homologous structures. What do these homologous structures show about the evolutionary relationship between the two species?

Answers

Two species have homologous structures. What do these homologous structures show about the evolutionary relationship between the two species? (3 points) The homologous structures show that the two species followed up the evolutionarypath up to the structure. They may have branched off right after that trait.

which life cycle stage is found in plants but not animals ​

Answers

Answer:

Multicellular haploid

OAmalOHopeO

Plants have multicellular haploid and multicellular diploid stages in their life cycle.

Gametes develop in the multicellular haploid gametophyte . Fertilization gives rise to a multicellular diploid sporophyte, which produces haploid spores via meiosis.What is multicellular haploid  stage?The haploid multicellular stage produces specialized haploid cells by mitosis that fuse to form a diploid zygote.The zygote undergoes meiosis to produce haploid spores. Each spore gives rise to a multicellular haploid organism by mitosis.

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In New York, the worst-hit state, a total of 350,121 cases have been confirmed and 28,232 deaths have been reported. There are now 29 states with tens of thousands of cases, and more than 50,000 in New York, New Jersey, Massachusetts, Illinois, California, Pennsylvania and Michigan

Answers

Answer:

Thats true

Explanation:

The two most important environmental factors affecting animals are __________ and __________. edtell

Answers

Explanation:

The two most important environmental factors affecting animals are biotic and abiotic.

Hope it helps you! \(^ᴥ^)/

The two most important environmental factors affecting animals are biotic and abiotic.

What is the Environment?

The environment may be defined as anything which is present in the surrounding of a living organism. The term environment was given by Carlyle.

Biotic factors include producers which are autotrophic in nature. Herbivores, Carnivores, omnivores, decomposers, parasites, etc.

Abiotic factors are generally long-lasting environmental conditions such as temperature, light, rain, fire, winds, etc., and topographic factors like mountains, hills, plains, desert, etc.

Therefore, the two most important environmental factors affecting animals are biotic and abiotic.

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Cells control or regulate the flux through metabolic pathways by means of I. allosteric control of enzymes. II. covalent modification of enzymes. III. genetic control of the concentrations of enzymes. IV. genetic expression of allosteric regulators.

Answers

Answer:

I, II, III

Explanation:

Allosteric regulation is a common mechanism of regulation of enzyme activity, which generally involves key enzymes in metabolic pathways. Allosteric modulation occurs when a substance/molecule called 'allosteric regulator' binds to an enzyme at a site other than the enzyme's active site, thereby triggering a conformational change in the enzyme and thus affecting its activity. Moreover, covalent modifications such as phosphorylation, acetylation, methylation, etc, are also involved in the regulation of enzymatic activity in metabolic pathways. For example, phosphorylation is a type of reversible covalent modification of proteins consisting of the addition of phosphate groups at specific amino acid residues (i.e., serine, threonine, and tyrosine) by specific enzymes known as protein kinases. Phosphorylation alters the three-dimensional (3D) structure of proteins, thereby turning the substrate or cellular pathway to active and/or inactive. Finally, both transcriptional and post-transcriptional mechanisms are also able to control the expression of enzymes involved in signaling pathways. Transcriptional mechanisms are capable of limiting the amount of mRNA that is produced from a given gene (in this case, an enzyme encoding gene), whereas post-transcriptional mechanisms such as, for example, RNA interference pathways, control the translation of messenger RNA (mRNA) molecules into proteins.

Compared with a eukaryotic cell, a prokaryotic cell Select one:______
a. lacks organelles beyond ribosomes.
b. is larger.
c. does not require energy.
d. is not living.
e. has no method of movement.

Answers

I believe A) would be the most correct answer in this case. However, the most significant difference between eukaryotes and prokaryotes is that eukaryotic cells contain a nucleus, while the latter does NOT.

B) is incorrect because prokaryotic cells are typically smaller than eukaryotic cells. Think bacteria and other microorganisms.

C) is incorrect because, well, energy is…

D) False, a non-living microorganism would be considered a prion or virus before prokaryote.

E) Many prokaryotic cells actually contain flagellum or cilia for transport.

Let me know if you have other questions — good luck.

The meaning of ALARA in radiation?

Answers

Answer:

The guiding principle of radiation safety is “ALARA”. ALARA stands for “as low as reasonably achievable”.

Leaves stalks flowers roots fruits and seeds are plant

Answers

Answer:

I'm confused as to what it is you are asking like are you asking or telling because I cant tell

Explanation:

what kind of mutation occurred when the DNA strand changed from AATCGTCCG TO AATAGTCCG?

Answers

Answer:

Missense Mutation

Explanation:

AAT CGT CCG to AAT AGT CCG where CGT has become AGT is a point mutation. If a point mutation changes the amino acid, it’s called a missense mutation. In this case CGT (arginine) has become AGT (serine).

some strains of __________________ produce proteins that kills certain insects such as lepidopterans , coleopterans and dipterans​

Answers

Answer:

Bacillus thuringiensis

Explanation:

some strains of Bacillus thuringiensis produce proteins that kills certain insects such as lepidopterans , coleopterans and dipterans​

Answer:

Bacillus thuringiensis

List 3 variable that Anurag should keep the same

Answers

Answer:

seawater ,upside-down funnel and seaweed

Explanation:

how do cells of plant devide without centrioles? explain​

Answers

Answer:

Because the spindle fibers originate outside the nuclear membrane, plant cells can divide without centrioles.

 ~OAmalOHopeO

identify the following organism and state to which phylum it belongs​

Answers

Answer:

it belongs to Coelenterata phylum.

Given the enormous heterogeneity of antigen receptors expressed on the populations of naive B and T lymphocytes, the adaptive immune response relies on a process whereby the rare lymphocyte that binds to the antigen is first induced to proliferate, before it can perform its effector function. For B cells, there is a clever mechanism that ensures that the specificity of the antibody secreted by the plasma cell will recognize the same pathogen that initially stimulated the B cell antigen receptor and induced B cell proliferation. This mechanism is:

Answers

Answer:

The naïve B cell expresses a membrane-bound form of the antibody as a receptor and secretes that same antibody when it differentiates into a plasma cell.

Explanation:

Naive B cell is a type of B cell that has still not been exposed to the antigen. The B cell receptors (BCRs) are transmembrane proteins composed of 1-a surface immunoglobulin molecule capable of recognizing the antigen, and 2-two transmembrane subunits that transduce the signal. When a B cell binds to antigen with its BCR, the B cell will proliferate and differentiate into a plasma cell. This plasma cell then releases antibodies, which are soluble forms of the BCR where the transmembrane domain that anchors the antibody protein to the membrane of the B cell is eliminated.

Cystathioninuria can be caused by two different mutations in the enzyme cystathionase. Cystathioninuria caused by mutation 1 can be overcome by providing cells with increasing concentrations of the coenzyme pyridoxal phosphate. Which of the following statements describe the most likely changes in the binding affinities of the two mutant enzymes?
A. Both mutant enzymes have lost the ability to bind the substrates.
B. The enzyme with mutation 1 has lost the ability to bind to the substrates, whereas the enzyme with mutation 2 has lost the ability to bind to pyridoxal phosphate.
C. The enzyme with mutation 1 has decreased affinity for pyridoxal phosphate, whereas the enzyme with mutation 2 has lost the ability to bind to the substrates.
D. Both mutant enzymes have lost the ability to bind pyridoxal phosphate.

Answers

Answer:

C. The enzyme with mutation 1 has decreased affinity for pyridoxal phosphate, whereas the enzyme with mutation 2 has lost the ability to bind to the substrates.

Explanation:

A coenzyme is an organic cofactor that binds with an enzyme in order to initiate or aid the function of the enzyme. A coenzyme binds to the active site of the enzyme (where the reaction occurs), thereby triggering its activation by modifying protein structure during the reaction. Some examples of coenzymes include Coenzyme A and Adenosine triphosphate (ATP). Pyridoxal phosphate is a coenzyme (it is the active form of vitamin B6) that is required for the function of cystathionase. Moreover, cystathionase is an enzyme that enables cells the synthesis of cysteine from methionine (transsulfuration pathway). The binding of pyridoxal phosphate to the enzyme increases the binding affinity of the enzyme for the substrate, thereby influencing its activity. In this case, it is expected that mutation 1 reduces the binding affinity of the enzyme to the cofactor, and thereby the cofactor is required at a higher concentration to restore normal enzyme activity.

What happens during rusting of an iron nail?

1 sulfur and oxygen combine to form a sulfur dioxide coating

2 iron and oxygen combine to form iron oxide

3 sulfur, iron, and oxygen combine to form iron sulfoxide

4 iron and sulfur combine to form iron sulfide

Answers

Answer: the correct answer is B number 2

Explanation: I just answered this question

What is a function of the integumentary system?

1 forms blood clots
2 grows new tissue
3 absorbs water
4 break down chemical bonds in food

Answers

Answer:

absorbs water is a function of the integumentary system. hope it help you.

A function of the integumentary system is to grow new tissues. Thus, the correct option B.

What is the Integumentary system?

The integumentary system may be defined as a unique type of system that primarily consists of the skin, hair, nails, and exocrine glands. It is the set of organs that construct an outermost layer of an animal or human body.

The integumentary system plays a significant role in the growth of new tissues within the body. The cells of the skin generally hold the mechanism of renewal or replacement of injured or damaged cells which are present on the epidermis part of a specific organ.

The integumentary system also protects the body from infections, and injuries provide defense against pathogens, etc.

Therefore, a function of the integumentary system is to grow new tissues. Thus, the correct option B.

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Why should body temperature not be allowed to fluctuate too much? ​

Answers

Answer:

Because that can destroy the helpful enzymes in the body, and therefore cause a lot of problems or maybe cause death

What is the fallopian tube fimbriae made of? Pls I need help asap

Answers

Answer:

The mucous membrane would be the answer

Phân tích các quy luật hoạt động thần kinh cấp cao ở trẻ và vận dụng trong thiết lập thói quen học tập và kỉ luật ở học sinh tiểu học.

Answers

Answer:

very different than ducks do u want it is not the

What is the fubtion of the nerves

Answers

Answer:

carry messages to and from the brain and spinal cord and the rest of the body

OR

it is a bundle of fibers that receives and sends message between the body and the brain

Answer:

The nervous system is the body's primary governing, regulating, and communication system. It is the hub of all mental activity, such as thinking, learning, and remembering. The nervous system, together with the endocrine system, is in charge of regulating and maintaining homeostasis.

OAmalOHopeO

What is the function of the mitochondria?
A. Stores the cell's DNA
B. Builds proteins
C. Produces energy for the cell by respiration
OD. Stores the cell's glucose
Reset Selection

Answers

C. Mitochondria is like the power plant of the cell and produces its energy.

Answer:

Produces energy for the cell by respiration

Explanation:

The glucose obtained from food is broken down to pyruvic acid in the cytoplasm. This pyruvic acid is broken down into oxygen, water and energy rich ATP molecules in the Mitochondria.

What variable should Anurag change in his experiment

Answers

Answer:

For geological carbon sequestration, the reaction of aqueous CO2 with silicate rock permits carbonate formation, achieving permanent carbon sequestration.A

Explanation:

Ecosystems rely on interdependence between species to keep balance. Which of the following is a threat to a stable
ecosystem?
A. Loss of biodiversity
B. High biodiversity
C. Low biodiversity
D. Increase in biodiversity

Answers

Answer:

loss of biodiversity

Explanation:

Biodiversity- refers to the variety of life on Earth at all its levels, from genes to ecosystems, and can encompass the evolutionary, ecological, and cultural processes that sustain life.

loss in biodiversity affect food chains greatly

thanks

hope it helps

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