Which of the following PCR modifications was developed to allow for the amplification of an RNA template ? A ) Multiplex B ) Nested C ) Reverse transcriptase D ) Real - time

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Answer 1

Transcriptase in reverse To enable the amplification of an RNA template, PCR modifications were created.

The relatively straightforward polymerase chain reaction (PCR) technology amplifies a DNA template to generate certain DNA fragments in vitro. Amplification of DNA sequences by PCR requires much less time than traditional procedures, which can take days or weeks to clone a DNA sequence into a vector and replicate it in a living cell.

Contrary to conventional biochemical tests, which include nucleic acid detection with radioisotopes, the PCR procedure only needs a little amount of biological material. As a result, PCR can amp up individual sequences at higher levels and with greater sensitivity than earlier techniques did.

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Related Questions

Place the biochemical name and enzymatic activity corresponding to each complex within the respiratory chain. Complex 1 Complex II Complex III Complex IV Answer Bank cytochrome bo, complex facilitates electron transfer from ubiquinol to cytochromec facilitates electron transfer from cytochrome o to 0, facilitates electron transfer from spyinate to ubiquinone succinate dehydrogeonse complex facilitates electron transfer from NADH to ubiquinone cytochrome oxidase NADH dehydrogenase complex

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The series of electron flow in the respiratory chain in the mitochondria membrane is-

NADH -------> Q --------> Cyt b ------> Cyt c1 --------> Cyt c -------> Cyt (a + a3) -------> O2

Complex I ; NADH dehydrogenase Complex Facilitated the electron transfer NADH to ubiquinone

Complex II; Succinate dehydrogenase Complex Facilitated the electron transfer succinate to ubiquinone

Complex III; Cytochrome bc1 complex or Ubiquinone: cytochrome c oxidoreductase Facilitated the electron transfer from ubiquinol to cytochrome c.

Complex VI; Cytochrome oxidase Facilitated the electron transfer from cytochrome c to O2​​​​​​.

In the electron transport chain, four protein complexes (designated complex I-IV) are involved in transporting electrons from NADH and FADH2 to molecular oxygen.

Complex I creates the hydrogen ion gradient by pumping four hydrogen ions from the matrix into the intermembranous region across the membrane.

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If a patient is having a hard time digesting a certain lipid known as triacylglycerol. What organ(s)
effect the digestion of this lipid? Why could this be happening?

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Triacylglycerol, also known as a fat molecule, is primarily digested by enzymes produced by the pancreas, called lipases. These enzymes break down the fat molecules into fatty acids and glycerol, which are then absorbed by the small intestine. The process of fat digestion also requires the presence of bile, which is produced by the liver and stored in the gallbladder. In some cases, a person may have difficulty digesting fats due to a lack of these enzymes or bile, which can be caused by conditions such as pancreatitis or gallbladder disease. Other factors that can affect fat digestion include certain medications, food intolerances, and diseases that affect the small intestine, such as Crohn's disease or celiac disease.

WILL MARK BRAINLIEST
The presence of the mineral iridium in Earth's rocks gives evidence to what events in Earth's history?

Question 3 options:

Solar storm activity


Increased volcanic eruptions


Asteroid impacts


Reversed polarity

Answers

The presence of the mineral iridium in Earth's rocks gives evidence to asteroid impacts. Option C

What is Iridium?

The element that is called iridium is one of the elements that are contained in the earth's crust. Many of the isotopes of iridium have been found to be radioactive. This is why we can be able to talk about iridium as we are looking at the history of the earth.

The mineral iridium is often present in large amounts and has been part of the geological history of the earth. It has taken part in all the earth events that have been taken place over the geological time scale of the earth and this explains why a study of the element is important as we are looking at the geological timescales of the earth in which we live and carry out activities.

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please select all of the locations where coccidioidomycosis is endemic.check all that apply semi-arid desert areas semi-arid desert areas the western hemisphere the western hemisphere the south atlantic states of the united states the south atlantic states of the united states the mississippi and ohio river drainage areas of the united statesthe mississippi and ohio river drainage areas of the united states

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The locations where coccidioidomycosis is endemic are a) Semi-arid deserts and b) The western hemisphere

Coccidioidomycosis is most common in the southwestern United States' semi-arid desert climate. Acute disease is brought on by the organisms being breathed in as spores, resulting in fever, chills, chest pain, hemoptysis, and dyspnea. The study of chest imaging reveals both condolidation and caitation, as well as widespread hilar lymphadenopathy. Patients with a previous infection are susceptible to reactivation and dissemination, regardless of their immunocompromised status. The semiarid regions of the United States have long been identified as the Coccidioidomycosi epidemic region, which is also located in the Western hemisphere. The activities that allow the fungus to enter the air and be inhaled by the susceptible host are the cause of the infection.

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Categorize the following neurotransmitter/neuromodulator as to its ef belong to more than one category fect on the postsynaptic neuron. Some neurotransmitters may GABA Excitatory Inhibitory Glutamate 00-59-47 Dopamine Glycine Norepinephrine Nitric Oxide Acetylcholine Substance P

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A neurotransmitter is a signaling molecule secreted by way of a neuron to have an effect on any other cell across a synapse. The mobile receiving the sign, any major body element or goal cellular, can be any other neuron, but could also be a gland or muscle cell.

Excitatory Inhibitatory

Glutamate - Helps in learning and memory. GABA - Reduces the Activity of neurons.

Dopamine - When it binds to D1 receptors Dopamine - When it binds to D2 receptors.

Glycine Glycine

Norepinephrine - Raises heartbeat and alertness.  

Nitric Oxide - Increases blood flow to the brain and reduces oxidative stress there.  

Acetylcholine - Activates the motor neurons to move and control muscles.  

Substance P - Activates the vomiting center in the Medulla, regulates mood disorders and stress in the body.  

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Which of the following are abiotic factors that determine the organisms that can exist in marine environments?
O amount of oxygen and salt concentration
O amount of carbon dioxide and amount of decomposers
O amount of moisture and variety of consumers
O amount of algae and range of temperature

Answers

Answer:

O amount of moisture and variety of consumers

Abiotic factors include sunlight, temperature, moisture, wind or water currents, soil type, and nutrient availability. 

What is the main benefit of an upwelling in the ocean?
OA.
OB.
O C.
OD.
It creates strong tides in coastal regions.
It increases the salinity of the water in an area.
It brings new nutrients into an underwater region.
It wipes out an ecosystem, allowing for secondary succession.

Answers

Answer: C.

Explanation:

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Besides increasing blood pressure and causing drinking, angiotensin II stimulates the release of ______, which is crucial to Na+ conservation

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Besides increasing blood pressure and causing drinking, angiotensin II stimulates the release of aldosterone.

A steroid hormone made by means of the adrenal cortex. It allows for managing the balance of water and salts within the kidney by using preserving sodium and liberating potassium from the frame. Too much aldosterone can purpose excessive blood strain and a build-up of fluid in body tissues.

In hyperaldosteronism, overproduction of the hormone aldosterone leads to fluid retention and improved blood pressure, weak point, and, rarely, periods of paralysis. Hyperaldosteronism can be a result of a tumor in the adrenal gland or can be a response to some illnesses.

Aldosterone reasons sodium to be absorbed and potassium to be excreted into the lumen through principal cells.

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A 21-year-old woman delivered a normal term infant a week ago and is now nursing the infant. She now notes a lump in her right axilla that has increased in size over the past week. On physical examination there is a rubbery, mobile, 1.5-cm mass beneath the skin at the right anterior axillary line. The mass is excised and the microscopic appearance is shown in the figure. Which of the following hormones most likely pro- duced the greatest effect upon this tissue?
A Cortisol
B Growth hormone
C Oxytocin
D Prolactin
E Testosterone

Answers

On physical examination there is a rubbery, mobile, 1.5-cm mass beneath the skin at the right anterior axillary line.

Oxytocin

The peptide hormone and neuropeptide oxytocin (Oxt or OT) is typically made in the hypothalamus and released by the posterior pituitary. It contributes to social interaction, reproduction, childbirth, and the time following childbirth. During labor and in response to sexual activity, the hormone oxytocin is released into the bloodstream. Additionally, it is accessible in pharmaceutical form. Oxytocin induces uterine contractions in both forms, hastening the labor process. In its natural state, it also contributes to the formation of the mother-baby relationship and milk production. A positive feedback loop governs the creation and release of oxytocin, with the first release of the hormone stimulating subsequent production and release. For instance, when oxytocin is released throughout a uterine contraction

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There are two types of systems: open and closed. An open system is one in which energy can be transferred between the system and its surroundings. The stovetop system is open because heat can be lost into the air. A closed system is one that cannot transfer energy to its surroundings. Biological organisms are open systems.

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Biological organisms are open systems. Through the consumption of energy-storing molecules and the release of energy to the environment through labor, they exchange energy with their surroundings.

Energy is governed by the rules of physics, just like everything else in the physical universe. The movement of energy inside and between all systems in the universe is governed by the principles of thermodynamics. Cells are biological systems on their own. One way to think about systems is as having some degree of order open systems. A system needs energy to be brought into order. The lower the entropy, the more organized a Biological organisms.

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T/F: Kelps with air bladders are common along the western and northwestern Pacific coasts of the United States

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Kelps with air bladders are common along the western and northwestern Pacific coasts of the United States it is a true statement

The primary moisture-bearing and rain-producing air mass throughout the year is the maritime Tropical (mT). It moves toward the pole in the winter and is cooled by the earth's surface. As a result, it has low stratus or stratocumulus clouds, drizzle, and poor visibility.

Due to the high-pressure areas that are prominent there, warm, humid maritime tropical (mT) air develops over tropical and subtropical ocean waters. On the other hand, just south and east of the substantial winter oceanic low-pressure regions, cool, moist maritime polar (mP) air develops over the colder subpolar ocean waters.

Humans have long been concerned with forecasting and predicting future weather conditions as a result of the variability of weather phenomena.

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The correct answer is True with air bladders are common along the western and northwestern Pacific coasts of the United States.

The middle intertidal zone is where the tides ebb and flow twice daily and where a wider range of flora and animals, such as sea stars and anemones, may be found. The intertidal zone is home to a variety of creatures, many of which have evolved to thrive in this continuously shifting environment. Mollusks (clams and mussels) and arthropods (crabs) have shells to prevent drying out and being crushed against the rocks by waves. TRUE: The majority of soft-bodied creatures and marine algae live in the low tide zone. The majority of creatures with shells are located in the spray zone.

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