g why did you digest the plasmid with XhoI? What is your expectation if the plasmid is a closed vector without an insert? What is your expectation if your plasmid has an insert (how many bands, what are their sizes)?

Answers

Answer 1

Answer:

I digest the plasmid with Xholin so as to find out the total size of the plasmid after insertion of the gene of interest and whether or not the insert went into the vector. Ao the plasmid is a reclosed vector without an put we expect to see one band. However, if the plasmid has an insert, we expect to see two bands on the gel


Related Questions

Please answer ASAP, the assignments in due really soon :) 30 Points!!! The image below shows the lac operon, which breaks down lactose in bacteria. Which statement correctly describes the expression of this gene?

Answers

Answer:

B. Gene expression is influenced by high lactose levels in the environment.

Explanation:

The lactose operon (lac operon) functions for the metabolism and transport of lactose in several bacteria.

Gene expression is influenced by high lactose levels in the environment as genes are expressed in the presence of high level lactose and absence of glucose. High level lactose carry an activator protein called catabolite activator protein (CAP), which let the RNA polymerase do transcription of the operon.

Hence, the correct option is "B".

Answer:

C

Explanation:

^he said it was right^

have an amazing day! :)

Select the scenarios that describe an abiotic factor that is density independent. Select the TWO answers that are correct.

a. Fir trees grow in number, increasing the amount of food available for moose
b. Heavy and continuous rains flood the land, submerging habitats.
c. Lightning strikes a tree and begins a fire that clears the land.
d. Ticks infesting moose weaken the moose's health and reproductive success.
e. Frigid temperatures flow into the area, causing hypothermia and death.

Answers

Answer: Option B and C.

Heavy and continuous rains flood the land, submerging habitats.

c. Lightning strikes a tree and begins a fire that clears the land.

Explanation:

Heavy and continuous rains flood the land, submerging habitats.

Lightning strikes a tree and begins a fire that clears the land.

The above are example of density independent abiotic factors because rainfall and lightning affect the population size of the environment by decreasing it's population sizes.

Abiotic factors are the non living part of the environment and they include rain fail, temperature,lightning, humidity e.t.c.

Density independent abiotic factors are factors that decrease or increase the population size of the ecosystem.

Therefore, Heavy and continuous rains flood the land, submerging habitats.

cLightning strikes a tree and begins a fire that clears the land are density independent factor because they decrease the population size.

The scenarios that describe an abiotic factor that is density independent are ; ( B and C )

Heavy and continuous  rains flood the land, submerging habitats ( B )Lightning strikes a tree and begins a fire that clears the land ( C )

Abiotic factors are Nonliving components of an ecosystem that has a significant effect on the ecosystem such as ; Rain, rocks, Temperature and humidity. Density Independent factors are factors in an ecosystem that can cause the decrease or increase of the population size of an ecosystem.

Heavy and continuous rainfall is an abiotic factor that has the ability to decrease the population of an ecosystem by submerging organisms habitats.

Lightning striking a tree and setting it ablaze which clears the land also reduces the population of the ecosystem because the fire will lead to loss of habitats and death of some organisms .

Hence we can conclude that The scenarios that describe an abiotic factor that is density independent are Heavy and continuous  rains flood the land, submerging habitats and Lightning strikes a tree and begins a fire that clears the land .

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explain the scientific theory of evolution and the evidence scientist found that proves it to be valid ?

Answers

Answer:

There are many theories

But main evidences are

Fossils of Plants animals and other organisms who lived on this planet at sometime. Oprain And Halden theory which suggests the chemical and biological evolution.

Which element provides strength to the exoskeletons of clams and oysters?
sulfur
oxygen
hydrogen
phosphorus​

Answers

Your answer is phosphorus

Please give brainliest

Phosphorus provides strength to the exoskeletons of clams and oysters. Thus, the correct option is D.

The exoskeleton of organisms including clams and oysters is made up of primarily calcium in the form of calcium phosphate and calcium carbonate.

Calcium phosphate is also present in the form of hydroxyapatite which is Ca₁₀(PO₄)₆(OH)₂ which provides invariable strength to the exoskeletal structure.

Thus, as phosphorus is a part of the exoskeleton, the correct option is D.

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Membrane-bounded vesicles that contain enzymes for oxidizing small organic molecules with the formation of hydrogen peroxide are Group of answer choices

Answers

Answer:

peroxisomes

Explanation:

The peroxisomes represent one of the organelles of the cell. They are usually small and characterized by membrane-bound vesicles that contain oxidative enzymes.

The enzymes in peroxisomes are involved in the oxidation of organic molecules - a process that leads to the formation of hydrogen peroxide. The later is further converted to water and oxygen by the constituent catalase enzyme of the peroxisomes. Fatty acids are also oxidized in peroxisomes but instead of hydrogen peroxide, acetyl groups that are utilized in other biosynthetic reactions are produced.

A student hypothesized that EC growth might be affected by the DNA from circulating erythrocytes. Is this student’s hypothesis reasonable?

Answers

Answer:

Explanation:

It can be said that this hypothesis is actually not reasonable because circulating erythrocytes do not contain DNA. This can be proven by the Mammalian erythrocytes (rbcs) which ultimately loose their nuclei as well as many other organelles during their maturation process and they don't have nuclei or mitochondria meaning that they are not affected by the DNA.

DIHYBRID CROSSES
In all of the examples we have only been concerned with one trait. It is possible, however, to track the inheritance of more than one trait at a time. When one is investigating the inheritance of two traits at once the cross is called a dihybrid cross. Strictly speaking, a dihybrid cross is one between two individuals that are heterozygous for two traits, meaning that they are hybrids in two senses. For example, in humans eye color is determined by multiple factors, but if we compare only blue eyes to brown eyes, brown is dominant (R) and blue acts as a recessive allele (r). If a man heterozygous for both eye color and bent little finger has a child with a woman who also heterozygous for both traits, this would be a dihybrid cross. Which is/are possible genotypes for a person with bent little finger and blue eyes?
1. bbrr
2. BBRR
3. BBrr
4. Bbrr
Referring to the previous question, which is/are possible genotypes for a person with straight little finger and blue eyes?
1. bbrr
2. BbRr
3. Bbrr
4. bbRr

Answers

Answer:

Question 1: bbrr

Question 2: bbRr

Explanation:

To address the first question, the possible genotype for someone with bent little finger and blue eyes would be bbrr.

Since brown eye (B) is dominant over blue eyes (b), in order for the blue eye color to be expressed, it has to be in bb condition. The only option with bb genotype is option 1.  

For the second question, the possible genotype for a person with a straight little finger and blue eyes would be bbRr.

Blue eye genotype is bb as explained in the first question while straight little finger would be Rr since the bent little finger has been represented by the genotype rr in the first question.

if a person who is blood type O marries a person who is blood type A, what are the possible blood types their children could have

Answers

Answer:

The possible blood types their children could have are A or 0.

Explanation:

  The I gene determines the blood type of a person. I is the dominant allele, while i is the recessive allele.

IA dominant over i. The person presents A type of antigens. IB dominant over i. The person presents B type of antigens. IA and IB are codominant. The person presents both types of antigens, A and B. i is the recessive allele. The person does not present any antigen.

Cross: a person who is blood type O with a person who is blood type A

Parental)    ii      x     IA-

Gametes) i   i          IA   -

Punnet Square)   IA            -

                     i      IAi           -i

                     i      IAi           -i

The - symbol represents the possibility of either IA allele or i allele. This is, the parental with blood type A might have the genotype IAIA or IAi

F1)  2/4 = 1/2 IAi

     2/4 = 1/2    -i  (-i could be either IAi or ii, depending on the genotype of the parental with blood type A)

Option 1: ii  x  IAIA

F1)  100% IAi (The whole progeny will have blood type A)

Option 2: ii  x  IAi

F1) 50% IAi (Half of the progeny will have blood type A)

    50% ii (Halfa of the progeny will have blood type 0)  

The possible blood types their children could have are A or 0.

A response to an unconditioned stimulus that occurs naturally without learning is known as a(n) __________ response.
A. neutral
B. conditioned
C. reconditioned
D. unconditioned

Answers

Answer:

it is going o be B. conditioned

Answer:

D

Explanation:

edge

When brown iodine is exposed to starch it turns dark purple. In an experiment, you placed a cornstarch solution in a small plastic bag. Next, you placed the bag in a beaker of water containing 10 drops of iodine. If the solution in the plastic bag turned dark purple (Select all that apply)
a) the plastic bag was permeable to cornstarch.
b) the plastic bag was permeable to iodine.
c) the iodine moved into the plastic bag.
d) the cornstarch moved into the beaker.
e) the plastic bag was selectively permeable.

Answers

Answer:

b) the plastic bag was permeable to iodine

the plastic bag was selectively permeable

the iodine moved into the plastic bag.

the plastic bag was permeable to cornstarch.

Explanation:

The experimental set up represented the concept of osmosis.That is  the movement of water  molecules from the region of higher  water potential to region of lower water potential through a semipermeable membrane(a  cell membrane which only allows water and certain molecules to pass through,but restrain other molecules through its pores.

Generally potassium iodine test is the standard test for starch.

Therefore, in the question,water moves iodine molecules as iodine solution(from high water potential) to move across the paper bag(semi permeable membrane) to the reach the corn solution.(low water potential,high solute potential).This turns the starch in the corn solution  blue-black/purple.

If the solution in the plastic bag turned dark purple:

the plastic bag was permeable to iodine. That is option (B)the iodine moved into the plastic bag.  That is option (C)the plastic bag was selectively permeable.  That is option (E)

To test for the presence of starch in a solution, iodine solution is used. This gives a blue-black coloration which occurred due to the formation of a starch-iodine complex.

From the above experiment, the plastic bag which contains the corn starch solution, separates the solution from water containing 10 drops of iodine.

The turning of the solution, inside the plastic bag, into blue black signifies the formation of starch-iodine complex between the cornstarch solution the iodine water.

The reaction was able to occur because the plastic bag, which is selectively permeable to iodine, allowed the movement of iodine from the beaker of water into it. This is called diffusion.

Therefore, if the solution in the plastic bag turned dark purple it means that the plastic bag which is semi permeable allowed the movement of iodine into it.

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a) There are three nucleotide in each codon, and each of these nucleotides can have one of four different bases. How many possible unique codons are there?
b) If DNA had only two types of bases instead of four, how long would codons need to be to specify all 20 amino acids?

Answers

Answer:

Explanation:

There are 64 possible codons to be specified for, If we have three nucleotides with 4 bases the set of all combinations can encode

4x4x4 = 64 amino acids

This will give 64 different combinations of four nucleotides

If we have 2 bases with 4 nucleotide we have a possibility of having 16 codons specifying for 20 amino acid.

If we have 2 nucleotides with 4 bases the set of combination is

4×4 = 16 amino acids of four nucleotides

If a person had not drunk any water for 8 hours before collecting a sample, would you expect his/her urine to have a high or low specific gravity? Why?

Answers

Answer:

The person’s urine will have a high specific gravity

Explanation:

A high specific gravity is characterized by an increased concentration of solutes in the urine of am individual. A low specific gravity is characterized by a low or decreased concentration of the solutes.This is usually due to dehydration as a result of environmental conditions or tedious physical activities.

This explains why a person who had not drank any water for 8 hours and is dehydrated before collecting a sample is thought to have a urine with a high specific gravity

Mitochondrial and chloroplast genomes typically Mitochondrial and chloroplast genomes typically encode some of the proteins needed for aerobic respiration and photosynthesis, respectively, as well as ribosomal and transfer RNA. encode for ribosomal and transfer RNAs. encode all of the proteins needed for aerobic respiration and photosynthesis, respectively. encode some of the proteins needed for aerobic respiration and photosynthesis, respectively. are composed of primarily noncoding DNA.

Answers

Answer:

The correct answer is "encode some of the proteins needed for aerobic respiration and photosynthesis, respectively, as well as ribosomal and transfer RNA".

Explanation:

Most organisms have a mitochondrial or a chloroplast genome in addition to the main genomic sequence located in the cell nucleus. The genes that are located in these organelles' genome include the genes that are used directly in the mitochondrion or chloroplast , including: proteins needed for aerobic respiration and photosynthesis (for instance genes involved in oxidative phosphorylation), respectively, as well as ribosomal and transfer RNA, which serve to the translation of proteins.

_is a molecule that directs all of a cells activities

Answers

Answer:

[tex]\boxed{\mathrm{Nucleus}}[/tex]

Explanation:

The nucleus directs all cellular activities. The nucleus also contains genetic material of eukaryotic organisms.

Which enzyme is used in gluconeogenesis, but NOT in glycolysis? Group of answer choices PEP carboxykinase enolase (interconverts 2-phosphoglycerate and phosphoenolpyruvate)

Answers

Answer:

PEP carboxykinase

Explanation:

PEP carboxykinase is also known as Phosphoenolpyruvate carboxykinase. It is an enzyme which is used for the production of glucose from non-carbohydrate carbon substrates. This enzyme is used only in gluconeogenesis not in glycolysis because this enzyme helps in the production of glucose while glycolysis is a process in which the glucose  break down into pyruvate and ATP molecules.

what is a radical a group of ions

Answers

Answer:

A radical is a group of atoms of elements carrying a charge. radicals or ions are formed by gaining or loosing ions. positive radical ions are called radical cations whereas negative radical ions are called radical  anions.

hope this helps you u :)      

Which process involves a decrease in the dispersal of matter? Select the correct answer below: heat exchange between two solids

Answers

Answer:

The options are

A.heat exchange between two solids

B.the decomposition of a compound into its constituent elements

C.the precipitation of a solid

D.none of the above

The answer is

C.the precipitation of a solid

Dispersal of matter involves the process whereby there is more space occupied by the resulting element/compound than the initial one. For example the conversion of liquid to gas means that it has an increase in the dispersal of matter as the gas particles will contain more space when compared to a liquid.

Precipitation of a solid means conversion of a liquid to a solid. A liquid is known to contain more space which means there is a decrease in the dispersal of matter.

The process that involved the reduction in the dispersal of matter should be the precipitation of a solid

What is the dispersal of matter?

It refers to the process in which it contains the additional space that should be occupied. Like the conversion of the liquid to gas represent there should be increment in the disperson of the matter since the particles of the gas comprise more space at the time when the comparison is to be done with the liquid. Here, precipitation of the solid represent the transformation of the liquid to the solid.

This question is incomplete. Please find the options below.

A.heat exchange between two solids

B.the decomposition of a compound into its constituent elements

C.the precipitation of a solid

D.none of the above

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Which is incorrect descriptions of the genetic event initiated by the HIV reverse transcriptase (RT) upon the HIV infection?
a. RT catalyzes initial synthesis of ssDNA using viral genomic RNA as the template.
b. The first RNA template is degraded after ssDNA synthesis.
c. The whole process is completed after synthesis of dsDNA.
d. tRNAs are adopted as the first primers.
e. None of these

Answers

Answer:

e. None of these

Explanation:

The immune deficiency viruses (HIV) are retroviruses that use a reverse transcriptase (RT) enzyme to produce a single-stranded DNA (ssDNA) from an RNA template. The reverse transcription allows retroviruses to replicate their genetic material, which is integrated into the host's genome as a double-stranded linear DNA molecule in a similar way to the mechanism of insertion used by endogenous retrotransposons. The synthesis of DNA is started by cellular tRNAs (tRNA3Lys) that are packaged into the virion. After reverse transcription, the HIV DNA enters the nucleus of CD4 immune cells (also known as CD4+ T cells), and then it integrates into the genome to coopt the host's cell machinery for its own replication.

Where does cellular respiration get
the energy to make ATP? *
A. Chemical energy from sugars
B.Light energy from the Sun
C.Energy stored in the covalent bonds of
water molecules
D.Energy stored in the covalent bonds of
carbon dioxide molecules

Answers

Answer:

I believe A.

Explanation:

Cellular respiration gets energy to make ATP from the food you eat, so, A.

Enzymes are protein molecules which are made up of long chains of

Answers

They are made up of amino acids

who is the father of Genetic​

Answers

Answer:

hey there

Explanation: answer is gregor mendal  is the father of genetic in 19th century.

Answer:

Gregor Johann Mendel

The lactic acid generated in the body of a cricketer is first converted to glycogen in 1) Muscles 2) Liver 3) Blood 4) Both 2 and 3

Answers

Answer:

2) liver

Explanation:

the bloods transports the lactic acid to the liver to be converted into glycogen

List three protozoan infections of the circulatory system that are transmitted by arthropods. Include the disease name, specific organism (genus species), vector that is the intermediate between hosts, and disease progress in humans.

Answers

Answer:

List three protozoan infections of the circulatory system that are transmitted by arthropods:

Malaria

Toxoplasmosis

Chagas disease (American trypanosomiasis)

Explanation:

Malaria is a prozotoan infection, that can be produced by several protozoan, in the genus Plasmodium. This Plasmodium infect red blood cells and the vector that trasmites it is the Anopheles mosquitoe. In this disease, red blood cells can have severe damage in different organs because of the hemolysis.

Toxoplasmosis is a disease caused by the protozoan Toxoplasma gondii. The main vector of them are domestic cats. When cats are infected, T. gondii oocysts is found in their feces and it can spread to humans. This disease is generally asymptomatic, except when the patient has AIDS or in pregnancy, which can be very dangerous and fatal.

Chagas disease, or American trypanosomiasis is caused by protozoan Trypanosoma cruzi and is generally transmitted to people thanks to the feces of triatomine bugs. Some of the acute symptoms include fever, vomiting, headache, and in severe cases people can develop a chronic disease. In the worst cases it can produce flaccid cardiomegaly (enlargement of the heart) leading to heart failure and death.

Which structures are highlighted? Which structures are highlighted? pulmonary arterioles alveoli bronchioles pulmonary capillaries

Answers

Hello. You forgot to put the image that complements this question. The image is attached below:

Answer:

pulmonary arterioles

Explanation:

Pulmonary arterioles are small blood vessels located in the lung. They appear as branches of the major arteries and have the main function of releasing blood from capillaries, in addition to regulating blood flow and peripheral blood pressure in capillaries.

It is important to note that arterioles are very small in size, which facilitates their functions to be performed efficiently and quickly.

During the 1800's, what country was a leader of world exploration and placed at the center of most maps?
A. Britain
B. Spain
C. Morocco
D. USA

Answers

Answer:

B. Spain

Explanation:

Portugal and Spain are the western European nations that were the primary players in the age of discovery in 18th century. Under the leadership of prince henry the navigator. With the spread of missionary activities, Christianity becomes the world's largest religion. Exploration of the Americas and other nations started from this place.

The force of a muscle contraction is higher when the muscle is stimulated every 0.5 secs than when stimulated with the same voltage every 0.05 seconds.

a. True
b. False

Answers

Answer:

b. False

Explanation:

Increasing the frequency of muscle stimulation causes wave summation to occur, which causes an overlap of muscle twitch. When this muscle summation happens, the force generated in the muscle increases. This means that stimulating the muscle every 0.5 seconds will generate less muscle contraction force than when stimulated every 0.05 seconds  

In humans, insulin is manufactured in the pancreas, insulin is a peptide hormone that causes the body’s cells to take up glucose. The blueprint for the polypeptide chain that is insulin is found in the — of specialized pancreatic cells.

Answers

Answer:

In type 1 diabetes the immune system erroneously attacks the beta cells that produce insulin in your pancreas. It causes permanent damage, leaving your pancreas unable to produce insulin. Exactly what triggers the immune system to do that isn't clear. Genetic and environmental factors may play a role.

Explanation:

Nucleic acids

Composed of Carbon, Hydrogen, Oxygen, Nitrogen and phosphorous.

DNA

The universal code that stores genetic information in all organisms

Semi

conservative-Bases in original strand split and new nucleotides complementary base pair to form two new strands

Mutations

Any change to the DNA sequence

Gene mutation

Permanent change in the DNA sequence that makes up a gene.

Transcription

The process of copying a nucleotide sequence of DNA to form a complementary strand of mRNA

Translation

Process by which mRNA is decoded and proteins are made.

Codon

Three nucleotide bases

tRNA

Reads the codon on the mRNA using anticodons and the complimentary base pair rules and delivers the amino acids

What would be the replicated DNA strand for the following DNA sequence?...GCGTATG

CGCATATC

What would be the mRNA and tRNA be for the following DNA sequence?...AGGTTA

mRNA...UCCAAU. tRNA...AGGUUA.

The process of converting the genetic message from DNA into ____ is called transcription.

mRNA

How many amino acids are in the human body and what do we call the bond that holds them together?

20; Peptide bond

A single strand of DNA contains the following nine nucleotides in order ACTTATGGA. What sequence of bases will be present on the complementary strand of DNA?

TGAATACCT

In humans, insulin is manufactured in the pancreas. Insulin is a peptide hormone that causes the body's cells to take up glucose. The blueprint for the polypeptide chain that is insulin is found in the __________ of specialized pancreatic cells.

DNA

Given the sequence of DNA nucleotide bases TTAGCCTTG, give the complementary mRNA produced during transcription.

AATCGGAAC

Identify the correct sequence of molecules, as a gene goes from coded information in the nucleus, to the product the cell wants to make.

DNA...messenger RNA...transfer RNA...Protein

Why is DNA replication considered semiconservative?

Each half of the old DNA strand serves as a template for the new growing strand causing the copied DNA to half new and half old.

Which is/are possible genotypes for a person with straight little finger and brown eyes?

a. bbrr
b. bbRR
c. BbRr
d. bbRr

Answers

Answer:

The correct answer is b. bbRR.

Explanation:

Brown eyes color is dominant over blue eyes color. So, we can assign the letter R to the dominant allele that expresses brown eyes and r to the recessive allele that expresses the blue eyes.  The phenotype bent little finger is dominant over the phenotype straight little finger. We can assign the letter B to the dominant allele, and b to the recessive allele.    

a. bbrr : This genotype belongs to a person with blue eyes and straight little finger

b. bbRR : This genotype belongs to a person with brown eyes and straight little finger

c. BbRr : This genotype belongs to a person with brown eyes and bent little finger

d. bbRr: This genotype belongs to a person with brown eyes and straight little finger.

Vaccination is a process of injecting a dead or weakened form of a pathogen into the body. How does this help strengthen the immune system? It allows the body to increase its internal temperature. It allows the body to regulate the inflammatory response. It allows the body to keep pathogen information for future infections. It allows the body to increase its desire to eat healthy food.

Answers

Answer:

It allows the body to keep pathogen information for future infections.

Explanation:

Vaccination is the process whereby a substance called VACCINE is injected into body in order to prepare the immune system for resistance against infections. A vaccine contains the harmless or dead version of a pathogen called ANTIGEN, which functions to give the immune system defense mechanism against that form of pathogen in future.

The vaccine works by stimulating the immune system of the body to fight against the antigens in the same way it will fight against an actual pathogen in the future. Hence, the body keeps the information on how to combat such pathogens when there is an actual infection.

Answer

The answer is C edge 2020

Explanation:

Which of the following accurately describes this element?

Answers

Answer:

Answer Expert Verified

The correct statements are: - Elements are made up of only one type of atom. - Each element has a unique chemical symbol. - Elements can be identified by their atomic number.

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