when sequencing a genome why do you need 50x coverage? (for example, a genome size of 1 billion nucleotides would need to have 50 billion nucleotides sequences to ensure adequate coverage)

Chemical contaminants that are frequently found in chemical dangers include toxins, antibiotics, and heavy metals from industrial discharges.

Heavy metals can be found in coal-burning power plants' combustion byproducts, metal piping used in industrial operations, untreated sewage sludge, and traffic. Pollution from toxicants is a significant environmental issue that has posed substantial risks to both agricultural productivity and human health.

Top of the list of environmental toxins harming nature are heavy metals and pesticides. Heavy metal contamination is seen by many as a problem in places with extensive industrial activity. Yet, today it is thought that one of the main sources of heavy metals is through roads and automobiles.

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Water molecules move through a membrane via a process known as osmosis.

Osmosis is the movement of water molecules from an area of lower concentration to an area of higher concentration. This means that water molecules move from an area with fewer molecules of water, to an area with more molecules of water. This is driven by the process of diffusion, where molecules naturally move from an area of higher energy to an area of lower energy. In osmosis, the energy of the water molecules is being used to overcome the energy of the membrane. This energy is used to break apart the membrane, allowing the water molecules to move through. As the water molecules move through the membrane, they continue to diffuse until they reach an area with an equal concentration of molecules. This process helps to maintain a balance of water molecules between the two sides of the membrane.

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First-strand synthesis of cDNA refers to the process of generating the first strand of cDNA from an RNA template.

This process typically involves reverse transcription, where an enzyme called reverse transcriptase synthesizes a complementary DNA strand to the RNA template.

1. RNA isolation: The RNA template is first isolated from the sample of interest. This may involve purifying the RNA from a biological sample using techniques like column chromatography or gel electrophoresis.

2. Reverse transcription: The isolated RNA is then used as a template for the synthesis of the first cDNA strand. Reverse transcriptase, an enzyme that can synthesize a complementary DNA strand from an RNA template, is used to catalyze this process.

3. Second-strand synthesis: Once the first cDNA strand is synthesized, a second complementary strand is generated through polymerase chain reaction (PCR) or other methods.

Overall, first-strand synthesis of cDNA is an essential process for studying gene expression and manipulating DNA sequences in the lab.

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The correct order of electron flow during photosynthesis begins at the top with the location where electrons are first excited in noncyclic electron flow and are as P680 → Cytochrome complex → Plastocyanin → P700 → NADP⁺.

Photosynthesis is the method by which organisms, mostly plants, generate organic compounds (like carbohydrates, fats, and proteins) from inorganic materials (water and carbon dioxide) utilizing the energy of light.

Photosynthesis can be divided into two stages: light-dependent reactions and light-independent reactions. The light-dependent reactions, which occur in the thylakoid membrane, are the first stage of photosynthesis.

Chlorophyll a absorbs light energy that energizes electrons, which are passed through two photosystems, PS II and PS I, as well as an electron transport chain. In this process, ATP is produced through the process of photophosphorylation.

The electrons are first excited in noncyclic electron flow at P680, electrons are then transferred to the cytochrome complex, which is then transferred to plastocyanin, then transferred to P700 and finally, electrons are then transferred to NADP⁺, completing the photosynthesis cycle.

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d) genetic drift. A change in the gene pool due to chance results in genetic drift.

Allele frequencies in a population can shift from generation to generation owing to random events, which is known as genetic drift. To be more precise, genetic drift is changed as a result of "sampling error" while choosing the alleles for the following generation from the gene pool of the current generation.

Contrary to natural selection, genetic drift uses a completely random method to alter the population's genetic composition. Genetic drift is a mechanism of evolution, but it does not lead to adaptations. Read more about genetic drift's mechanism and sampling mistakes.

Hence, environmental factors can have an impact on or act as a mediator of genetic drift and gene flow, which are neutral or non-adaptive evolutionary processes.

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RNA polymerase (green), an enzyme involved in transcription, creates pre-mRNA by using DNA as a template (pink). Pre-mRNA undergoes processing to create a mature mRNA molecule that can be translated to create the protein molecule.

Polypeptide that the original gene intended. A promoter sequence at the start of a gene is where transcription starts when RNA polymerase binds to it (directly or through helper proteins). A new, complementary RNA molecule is created by RNA polymerase using the template strand of one of the DNA strands as a model.

A procedure known as termination marks the end of transcription. Beginning, continuing, and ending are the three phases of transcription. After transcription, RNA molecules in eukaryotes must undergo synthesis processing, which includes splicing, adding a 5' cap and a poly-A tail to either end, and other steps.

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Correct Question:

What is the process of transcription in protein synthesis?

Of the three outcomes of hybridization, the outcome that will result in the continued generation of interspecies hybrids is known as introgressive hybridization.

Hybridization is a biological process in which two species with different genetic characteristics are cross-bred to create a hybrid. It can occur naturally or as a result of human intervention. In nature, hybridization usually occurs between related species or different subspecies, resulting in fertile offspring.

Introgressive hybridization: This is the outcome of hybridization that will result in the continued generation of interspecies hybrids. Introgressive hybridization occurs when hybrids mate with one of the parental species or with other hybrids. The introgression process allows for the transfer of genes from one species to another or from a hybrid back to a parent species. This leads to a genetic transfer that creates new lineages that differ from the parental species.

Introgressive hybridization is a rare phenomenon, but it has been observed in a number of animal and plant species. It is usually the result of natural hybridization, but it can also be facilitated by humans, especially in agriculture and horticulture. The process can have both positive and negative consequences, depending on the specific species involved and the environment in which they occur.

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The push-up is an effective bodyweight workout that targets many upper body muscles. When you do a push-up, you use numerous muscles in your upper body. However, the three primary muscles are the chest, triceps, and shoulders.

Chest muscles when you do a push-up, the chest muscles are the main muscle group targeted. The pectoralis major and pectoralis minor muscles are the main muscles involved in the push-up. These muscles aid in shoulder extension and flexion, as well as arm adduction and abduction. Shoulder muscles when you do a push-up, the muscles in your shoulders are also worked. The primary muscles are the deltoid muscles. The deltoid muscle is a three-part muscle located on the shoulder. The front, middle, and rear deltoid muscles all work together to raise and lower your body.

The anterior deltoid muscles are activated when you lower your body, while the posterior deltoid muscles are activated when you push your body back up. Tricep muscles when you do a push-up, the tricep muscles are also engaged. The triceps are located at the back of the upper arm, and they are responsible for extending the elbow joint. When you lower your body during a push-up, the triceps contract eccentrically, which means that the muscle fibers lengthen as they contract. When you push your body back up, the triceps contract concentrically, which means that the muscle fibers shorten as they contract. In conclusion, the primary muscles used during a push-up are the chest, shoulders, and triceps. However, the muscles of the back, core, and legs also play a supporting role in maintaining proper form and stability.

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High ATP yield; most from oxidative phosphorylation: This characteristic is specific to respiration. In respiration, the electron transport chain is involved in the generation of ATP, and most of the ATP is produced through oxidative phosphorylation.

Most carbon released as CO2: This characteristic is specific to respiration. During respiration, the carbon from the organic molecules is completely oxidized, and most of it is released as CO2.

Electrons reduce an external electron acceptor (e.g., O2): This characteristic is specific to respiration. In respiration, oxygen or other external electron acceptors act as the final electron acceptors, and electrons are transferred to them during the electron transport chain.

Low ATP yield; primarily from glycolysis: This characteristic is specific to fermentation. In fermentation, ATP is primarily produced through glycolysis, which has a low ATP yield.

Electrons from catabolism end up back on partially oxidized substrate: This characteristic is specific to fermentation. In fermentation, the electrons from the catabolism of organic molecules end up back on partially oxidized substrates such as pyruvate or acetaldehyde.

Most carbon remains in organic form: This characteristic is specific to fermentation. In fermentation, most of the carbon from the organic molecules remains in organic form, and only a small amount is released as CO2.

Important for catabolism of sugars: This characteristic applies to both respiration and fermentation. Both processes are important for the catabolism of sugars, which involves the breakdown of complex organic molecules to release energy.

NADH major electron carrier: This characteristic applies to both respiration and fermentation. In both processes, NADH is an important electron carrier, which plays a crucial role in transferring electrons during the breakdown of organic molecules.

Pyruvate is major intermediate: This characteristic applies to both respiration and fermentation. In both processes, pyruvate is a major intermediate, which is produced during glycolysis and serves as a precursor for further metabolic pathways.

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The complement system is a group of proteins that form part of the immune system.

It works to fight infection and other diseases by enhancing the body's natural immune response. It does this by increasing inflammation, promoting the destruction of invading cells, and by activating the clotting cascade to seal off the infection. The complement system is made up of more than 20 different proteins, which are divided into three main categories: the classical, lectin, and alternative pathways. The classical pathway is triggered by antigen-antibody complexes, while the lectin and alternative pathways are triggered by bacterial components such as lipopolysaccharide. The classical pathway activates the cascade of proteins and chemicals which leads to the destruction of the invading cells. The lectin and alternative pathways activate the production of the anaphylatoxins C3a and C5a, which are important mediators of the inflammatory response. The C3a and C5a molecules bind to the surface of invading cells, triggering their destruction. The complement system also helps activate the clotting cascade, which seals off the infection. Ultimately, the complement system helps to fight infection and other diseases by increasing inflammation and promoting the destruction of invading cells.

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The central nervous system (CNS) is composed of the brain and spinal cord and is responsible for coordinating and controlling body functions. The peripheral nervous system (PNS) is composed of nerves and ganglia outside of the brain and spinal cord and is responsible for connecting the CNS to the rest of the body.

Motor neurons are responsible for transmitting signals from the CNS to muscles and other effector organs to produce a response. Sensory neurons are responsible for transmitting signals from sensory organs and receptors to the CNS.

An axon is a long, slender projection of a nerve cell, or neuron, that conducts electrical impulses away from the neuron's cell body. A dendrite is a short, branching projection of a nerve cell that conducts electrical impulses toward the cell body.

Proteins need to be denatured using an anionic detergent such as SDS before gel electrophoresis because:

a) most proteins have a uniform positive charge,

b) the overall charge of a protein depends on the amino acid composition of the protein and can vary greatly, and

c) most proteins have complex three dimensional structures that can impact their movement through the gel.

Denaturing proteins with an anionic detergent like SDS neutralizes the charge of the proteins, allowing them to move evenly through the gel in a consistent pattern during the electrophoresis process.

Without the anionic detergent, the proteins would not move evenly and the results of the gel electrophoresis would not be accurate.

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The general term used to designate problems resulting from damage to or disease of any components of a motor unit, including the somatic motor neuron, neuromuscular junction, and muscle fiber, is known as 'motor unit pathology'.

Neuromuscular disorder is a condition that affects muscles, nerves, and their interaction. It can cause muscle weakness, twitching, pain, and cramps, as well as breathing or swallowing difficulties. There are a variety of neuromuscular disorders, each with its own causes, symptoms, and treatments. Some of the most common neuromuscular disorders include muscular dystrophy, amyotrophic lateral sclerosis, multiple sclerosis, and myasthenia gravis. A muscle disorder refers to any disorder that affects the functioning of the muscles in the body.

This may involve a range of diseases, from myopathies, which are disorders that affect the muscles themselves, to neuromuscular disorders, which affect the interaction between the muscles and nerves that control them. As a result, muscle disorders may cause muscle pain, weakness, and stiffness, as well as a range of other symptoms.

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Peptidoglycan is present in the cell walls of: option (D) which is "eubacteria"

Peptidoglycan is a molecule that makes up the cell walls of most bacteria. This makes it an excellent target for the development of antibiotics that specifically target bacteria but not other organisms since no other organisms have peptidoglycan in their cell walls.

Peptidoglycan is a polysaccharide and peptide complex that makes up the majority of bacterial cell walls. In the cell walls of gram-negative bacteria, it forms a thin, protective layer around the plasma membrane, while in the cell walls of gram-positive bacteria, it makes up a much thicker, more extensive layer.

It provides the bacteria with structural support and protects it from osmotic pressure changes that would otherwise cause the cell to burst or shrivel up. The presence of peptidoglycan in bacteria cell walls provides the basis for Gram staining, a technique used to differentiate bacterial species. Peptidoglycan is not present in the cell walls of any other type of organism except eubacteria.

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Answer:

Yes one A and one B can make an O

Yes, a mother with blood type A and a father with blood type B can give birth to a son with blood type O.

The best explanation of the processes illustrated in Figure 1 is that the introns are removed from the pre-rRNA, and the mature rRNA molecules are joined and then translated to produce the protein portion of the ribosome.

In other words, sections of the pre-rRNA are removed and the mature rRNA molecules are available to combine with proteins to form the ribosomal subunits, which then get translated to create the proteins that make up the ribosomal subunits.

The introns are also known as intervening sequences since they are not observed in mature RNA. rRNA or the ribosomal RNA plays an important structural as well as catalytic role during the process of translation.

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Calvin cycle or the light-independent reactions are responsible for carbon fixation, taking inorganic carbon dioxide gas and converting it into organic molecules that the plant can use as energy.  

The correct options are a. Plants would not produce oxygen. b. G3P would be produced in much smaller quantities. c. NADPH production would decrease while ATP production increased.

In photosynthesis, the light-dependent reactions happen in the thylakoid membranes of the chloroplasts in a plant cell. These reactions create the energy currency for the plant cell in the form of ATP and NADPH. They additionally create oxygen as a byproduct.

The ATP and NADPH made by the light-dependent reactions at that point power the light-independent reactions. They occur in the stroma of the chloroplasts of the plant cells.

The light-independent reactions use ATP and NADPH that were created by the light-dependent reactions to power the conversion of CO2 into G3P (glyceraldehyde 3-phosphate), a carbohydrate molecule.

If plants were no longer able to perform the light reactions and they could only utilize the Calvin cycle, the following would be true: Plants would not produce oxygen because oxygen is produced as a byproduct of the light-dependent reactions, which are no longer occurring.G3P would be produced in much smaller quantities.

This is because ATP and NADPH that were created by the light-dependent reactions are required for the conversion of CO2 into G3P. Without the light-dependent reactions, the amount of G3P produced would decrease significantly. NADPH production would decrease while ATP production increased.

ATP would be produced through the cyclic electron transport pathway that makes use of Photosystem I only. It will produce ATP but not NADPH. As a result, the amount of ATP created would increase, while the amount of NADPH would decrease.G3P would be produced in much greater quantities.

This is incorrect, because, as mentioned above, the amount of G3P produced would actually decrease because the light-dependent reactions, which create ATP and NADPH, are not happening.

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""Smashing into the car" is a phrase. It does not express a complete thought and cannot stand alone as a sentence.

It typically includes a subject, which is the person, place, thing, or idea the sentence is about, and a predicate, which is the action or description that is being performed or stated about the subject. A sentence can be simple, containing just one independent clause, or it can be complex, containing multiple clauses joined together. Sentences can be used to make statements, ask questions, give commands, or express strong emotions.

As a verb, "smashing" means to break or crush something violently into small pieces.

As an adjective, "smashing" means excellent, impressive, or attractive.

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The restriction enzymes will cut straight through both strands of DNA is a statement that is not entirely correct. Restriction enzymes recognize specific DNA sequences and cut the DNA in a particular way.  Therefore, the correct answer is option A.

Some of them will cut straight through, while others will leave an overhang at both ends of the cut. However, some of them cut straight through both strands of DNA. In contrast, others will cut through the DNA, leaving some overhang at both ends. Restriction enzymes are nucleases that cut DNA molecules into smaller pieces. These enzymes play a crucial role in genetic engineering as they allow scientists to manipulate DNA molecules in the laboratory. The enzymes recognize specific DNA sequences, and this ability allows them to cut the DNA at precise locations. Some of the restriction enzymes cut the DNA straight through both strands, while others cut through the DNA but leave overhangs at both ends. The overhangs can be used to join the cut DNA fragments with other fragments through a process called ligation.

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If the student repeats the experiment with a different substrate and measures no enzyme activity then this would happen because the enzyme cannot bind to the new substrate.

The expression enzyme specificity in terms of enzymatic activity means that one enzyme only can bind to a group of related substrates while it does not bind to other molecules.

Therefore, with this data, we can see that enzyme specificity in terms of enzymatic activity is capable of explaining the role of enzymes in the cell.

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Areas of the body with a lot of lymphocytes are also included in the lymphatic system. They are referred to as lymphatic tissue.

Patches by Peyer: The mucous membrane that lines your small intestine contains these little clusters of lymphatic tissue. These lymphoid cells monitor and get rid of intestinal microbes. Appendix: Your appendix contains lymphoid tissue that has the capacity to get rid of bacteria before they enter the gut wall during absorption.

The diffuse lymphatic tissue that is present throughout the mucosa and submucosa, occasionally containing dispersed nodules, is the key diagnostic characteristic of the appendix. The appendix's lumen frequently contains debris.

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B. A feature or behavior that helps an animal survive in its environment.

Yes, the thick-walled structures you are examining could be from bacteria. Bacteria can form these structures as part of their life cycle, and they become dormant during extreme weather conditions, like freezing cold winters and hot dry summers
When you are examining samples from a site in a dry pond that alternates between freezing cold winters and hot dry summers and you find dormant thick-walled structures, it is likely that these are from bacteria and are spores.

Spores are small, sturdy, dormant bacterial cells that can remain viable for lengthy periods of time, ranging from years to decades. During periods of adverse environmental conditions, such as drought or extreme temperatures, bacteria convert themselves into spores, which are capable of surviving until conditions improve. Bacteria are able to survive in adverse conditions such as drying out or freezing by forming spores.

Endospores are the thick-walled spores that some bacteria generate when environmental conditions become too harsh for them to grow. During periods of adverse environmental conditions, such as drought or extreme temperatures, bacteria convert themselves into spores, which are capable of surviving until conditions improve. Spores of bacteria are primarily created by gram-positive bacteria.A bacterium that is made up of endospores can easily survive boiling water, harsh chemicals, and other adverse environmental conditions that would otherwise destroy it. The spore-forming capability of bacteria is a survival mechanism that enables them to survive in hostile conditions until favorable conditions arise for them to begin to grow again. As a result, spore-forming bacteria are among the most resilient and tenacious types of bacteria in existence.

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The depolarizing current causes a burst of electrical activity called an action potential. This indicates that due to an action (stimulus), the resting potential becomes 0 mV.

The sodium/potassium pump uses ATP to transport 3 sodium ions out of the cell and 2 potassium ions into the cell, thus maintaining the resting membrane potential at the axon. This keeps the apparent negative charge of the neuron in place. The sodium-potassium pump sequentially removes three sodium ions from the cell for every two potassium ions introduced.

As such, it retains the significant gradient of potassium ions across the membrane that underlies the resting membrane potential.

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The girl's displacement from her starting point to the pet store is √26 blocks, approximately 5.1 blocks and the distance she walked in blocks is 11 blocks

To find the girl's displacement from her starting point to the pet store, we need to find the net distance and direction from her starting point to the pet store.

Starting from her home, she walked 3 blocks north, then 2 blocks east, then 1 block north, then 1 block east, and finally 4 blocks south. The net displacement can be found using the Pythagorean theorem:

Net displacement = √(3² + 1² + 4²)

Net displacement = √26

Net displacement = 5.1 blocks.

The girl's displacement from her starting point to the pet store is √26 blocks, approximately 5.1 blocks.

The location of the pet store in relation to her home can be described as 2 blocks south and 1 block east.

To calculate the distance she walked in blocks, we can add up the distances of all the legs of her journey:

Distance = 3 + 2 + 1 + 1 + 4

Distance = 11 blocks

The girl walked a total of 11 blocks.

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In Guam, the brown tree snake is an invasive species that has caused a dramatic decline in biodiversity.

An invasive species is an animal or plant that is introduced to an ecosystem and causes damage to the natural environment. They could be brought to new areas as a result of human activities, such as the introduction of foreign species in shipping ballast or the use of non-native species in landscaping.

Brown tree snake in Guam: Brown tree snakes are a non-native species that were first introduced to Guam in the 1940s. It was unintentionally brought to the island as a stowaway on ships from Southeast Asia. Brown tree snakes have been thriving in the island because they do not have any natural predators in Guam, which has led to a decline in biodiversity as well as causing significant environmental and economic damages.

Invasive species like the brown tree snake are known for their destructive tendencies, and they have a significant impact on local ecosystems. They can damage habitats, reduce biodiversity, and cause economic losses.

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A peptide bond between two amino acids is created when the carboxyl group (-COOH) of the first amino acid binds with the amino group (-NH2) of the second amino acid. This is a type of covalent bond that joins two amino acids together to form a protein or polypeptide chain.

The formation of a peptide bond involves the removal of a water molecule, also known as dehydration synthesis. During this process, the amino group of one amino acid combines with the carboxyl group of another, and the resulting molecule is a dipeptide. Amino acids are the building blocks of proteins. The human body uses 20 different types of amino acids to build proteins. These amino acids have different side chains, which determine their properties and function. The sequence and arrangement of amino acids in a protein determine its structure and function.

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The change in blood vessel length had a direct effect on flow rate. As the length of the blood vessel increased, the flow rate decreased.

The effect of the change in blood vessel length on the flow rate can be explained as follows: As per the Poiseuille's Law, the resistance to blood flow in a vessel depends on several factors such as the length of the vessel, the viscosity of the blood, the diameter of the vessel, and the pressure gradient. The formula for Poiseuille's law is given as follows:

R = 8 × η × L / (π × r⁴)

Where, R = resistance to flow, η = viscosity of the blood, L = length of the vessel, r = radius of the vessel.

From the above formula, it is evident that resistance is directly proportional to the length of the vessel. Therefore, when the length of the blood vessel increases, the resistance to flow increases as well. Hence, the flow rate decreases. This explanation can be used to predict the effect of the change in blood vessel length on the flow rate. The results of the experiment can then be compared with the predictions made using the above explanation.

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The given statement is False. For any given species, cells in metaphase II of meiosis would contain half of the genetic material than cells in metaphase of mitosis.

Mitosis is a process in which a single cell divides into two genetically identical daughter cells. During mitosis, the cell nucleus and cytoplasm both divide to form two daughter cells. Mitosis is responsible for asexual reproduction in unicellular organisms and for the growth and repair of tissues in multicellular organisms.

Meiosis is a process in which a diploid cell divides into four haploid daughter cells, each with half the number of chromosomes as the parent cell. Meiosis produces gametes for sexual reproduction. Each chromosome has two sister chromatids and two homologous chromosomes.

Metaphase is the stage of mitosis in which the chromosomes align along the metaphase plate. The spindle fibers attach to the kinetochores of the chromosomes, and the chromosomes are pulled to the centre of the cell.

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