Using sophisticated molecular cloning techniques, you have isolated the genes for two serotonin transporters, called TransA and TransB, which are expressed in these neuronal cells. A preliminary immunofluores- cence localization of fixed and permeabilized cells found very bright staining of TransA at the nerve cell plasma membrane and little staining elsewhere. TransB, in contrast, stained only very faintly at the nerve cell plasma membrane, but there was intense staining within the cytosol.

Required:
In which subcellular membrane or organelle would you expect most of the TransB to be found? Explain your answer.

Answers

Answer 1

Answer:

Vesicles  

Explanation:

Serotonin is a neurotransmitter that transports signals or messages between neurons.

Most neuronal cells have vesicles in their interior, which are organelles that store neurotransmitters for exportation -by exocytosis- or from recycling -by endocytosis-. These vesicles also protect the neurotransmitter from the enzymatic action.

Vesicles form in the cellular soma, from where they are transported to nervous terminals. Once the vesicle releases the neurotransmitter to the intercellular space, their membrane remains available in the plasmatic membrane to be reused.

The neurotransmitter concentration in the vesicle interior is related to the storage system and the transport system. There are specialized transporter proteins in the vesicle membrane that are involved with the introduction of the molecule to the organelle.

In the exposed example, transporter Trans B might be located in the vesicle membranes, and hence could be found in the cytosol of the cells.


Related Questions

Describe how and where viruses reproduce and the function of RNA and DNA in this process.

Answers

Answer:

Using the host's cellular metabolism, the viral DNA begins to replicate and form proteins. Then fully formed viruses assemble. These viruses break, or lyse, the cell and spread to other cells to continue the cycle. Like the lytic cycle, in the lysogenic cycle the virus attaches to the host cell and injects its DNA.

Normal diploid somatic cells of the mosquito Culex pipiens contain six chromosomes. The G1 nucleus of a mosquito cell contains 3.0 x 10^-12 grams of DNA. How much nuclear DNA would be expected in metaphase I of meiosis?
a. 6.0 x 10^-12 g.
b. 1.5 x 10^-12 g.
c. 12 x 10^-12 g.
d. 3.0 x 10^-12 g.
e. 0.75 x 10^-12 g.

Answers

Answer:

a. 6.0 x 10^-12 g.

Explanation:

The interphase is the previous step before the cell divission occurs. The interphase is formed of the G1, S, and G2 stages.  

• During the G1 stage, it occurs a high intense biochemical activity. The cell duplicates its size, and the organelles and other molecules and cytoplasmatic structures duplicate too. Some structures, such as microtubules and actin filaments, are synthesized from zero. The endoplasmic reticulum increases in size and produces a membrane for the Golgi apparatus and vacuoles, lysosomes, and vesicles. During this stage, the pair of centrioles separate, and each centriole duplicates. Mitochondria and chloroplasts also replicate.  

During the S stage occurs the DNI replication process. It also occurs the synthesis of histones and other associated proteins. This is the only stage where the DNI molecule is replicated.

• G2 stage is the final one before the cellular division. Here begins the slow process of DNI condensation. Duplication of centrioles completes. Structures such as spindle fibers are assembled.  

Through the process of Meiosis, a diploid germ cell (2n) divides and originates four daughter cells with a haploid chromosome number (n). Each daughter cell has half of the chromosomes of the original one. Meiosis is completed in two phases. During the first phase, and after replication, occurs the chromosome´s reduction division. During the second phase, the cell suffers a new, not reductive division.

1. In the first phase, Meiosis I:  

Prophase I: Chromosomes condensate and became visible. Occurs crossing-over between homologous chromosomes. Crossin-over makes the daughter cells to be genetically different from the original one.  Metaphase I: The pairs of homologous chromosomes randomly align in the equatorial plane.  Anaphase I: occurs the independent separation of homologous chromosomes that migrate to opposite poles of the cell. This separation generates different chromosomal combinations in the daughter cells.  Telophase I: Each of the homologous pairs chromosomes is already in the corresponding poles, and the nuclear membrane forms again in each pole.  

2. In the second phase, Meiosis II:  

Prophase II: Chromosomes condensate again and become visible.  Metaphase II: Chromosomes join the spindle apparatus and migrate to the equatorial plane, where they randomly line up. Sister chromatids are holden together until they reach the Anaphase.  Anaphase II: Centromeres divide, chromatids get separated, and each of them goes forward an opposite cellular pole.  Telophase II: Once in the poles, the chromosomes became lax again, and cytokinesis occurs.  

In the exposed example, DNI in the G1 nucleus of a mosquito cell still has not suffered replication (3.0 x 10^-12 grams). The replication process occurs during the S stage, which follows the G1 stage. After the S stage, the cell has 6.0 x 10^-12 g of DNI.

During meiosis, the reduction in chromosomes number occurs in anaphase where homologous chromosomes migrate to different poles. But during metaphase I, the cell still has 6.0 x 10^-12 g of DNI.

Writing an Equation in Slope-Intercept Form
Q
Check
y
Determine the equation for the given line in
slope-intercept form
4
O y = -5x-1
=-1
2
X
-6
-4
-2
2
LO
O y = x+1
O y = 3x+1
O y=-x-1
-2
-4
Hint

Answers

Answer:

need a full picture plzzz

Fill in the blanks: Antibodies are produced by _______________________________________ and bind to specific ________________________________ on erythrocytes, causing ________________________________, or clumping of erythrocytes.

Answers

Answer:

Antibodies are produced by _white cells__ and bind to specific _antigens_ on erythrocytes, causing __agglutination__, or clumping of erythrocytes.

Explanation:  

Macrophages are one of the different types of cells that intervene in the organism's defense system against strange substances and pathogen agents. These cells are the principal actors in the immune response.

Macrophages have several functions. They are the principal phagocytes of tissues, capable of recognizing different strange molecules that penetrate the organism, such as bacterias, parasites and, viruses. Macrophages phagocyte these substances and eliminate them, a process known as phagocytosis. When macrophages are activated, they release cytokines that favor inflammation response, which is used to neutralize the pathogen agent. When macrophages phagocyte strange substances, they show the antigens in their surfaces to be recognized by lymphocytes.

Antigens are defined as the strange substances that enter the organism and trigger a series of cellular events that produce defense mechanisms. Antibodies recognize antigens as invaders.  

In the organism, there are leucocytes or lymphocytes (white cells) in charge of immune defense. These are B cells, which produce antibodies, and T cells that can destroy infected cells from the body. They all circulate in the blood.

Antibodies are globular proteins produced by lymphocytes in response to the presence of strange molecules. Each type of antibody recognizes and combines with a particular antigen, immobilizing it. After that, the antigen is destroyed by other components of the immune system.

Take the gram seeds. Divide the seeds into three sets A, B and C.
➢ Put the seeds of set A onto the moist cotton.
➢ Soak the seeds of set B in the water overnight and then put on the moist cotton for seed germination.
➢ Put the seeds of set C in the boiling water for some time then allow it to germinate on the moist cotton.
➢ Grow a plant in another set.
➢ Note the observations every week.
Set A
Set B
Set C
Plant 1 st week 2 nd week So on….

Answers

Explanation:

set A ,seeds will germinate

set B,seeds will rot due to the presence of alot of water

set C,seeds will not germinate

based on the 1:2:1 proportions of carbon, hydrogen and oxygen, it can be determined that a glucose molecule is a

Answers

Answer:

Carbohydrate (monosaccharide)

Explanation:

Carbohydrates are one of the four biological molecules in nature. Carbohydrates are structurally composed of carbon, hydrogen and oxygen elements. However, these elements (C,H,O) are present in each carbohydrate in the ratio 1:2:1 respectively.

As stated in this question, glucose is an organic molecule made up of carbon, hydrogen and oxygen in the 1:2:1 proportion, hence, it is a CARBOHYDRATE. Since glucose possess one sugar component, it is classified as a MONOSACCHARIDE with a chemical formula C6H12O6.

P is an autosomal dominant allele that produces a pigment, p is a recessive allele that produces no pigment. A pigmented and a non-pigmented organism mate and produce a pigmented offspring. They then mate and produce a non-pigmented offspring. What is the probability the next offspring will be pigmented

Answers

Answer:3/4

Explanation:4 possibilities, 3 dominant

If a pigmented and non-pigmented organism mate and produce a pigmented organism, which mate again and produce non-pigmented organisms, the probability that the next offspring will be pigmented is ¾ as P is the dominant, pigment-producing allele.

What are dominant alleles?

The association between two genetic variants is referred to as dominant. Each gene has two alleles that an individual inherits from each parent. One allele of a gene, known as the dominant gene, will be expressed if the alleles are different. The impact of the additional allele, known as the recessive one, is concealed.

If one is in charge, the other must be recessive. Then it is referred to as recessive. A dominant gene, or dominant form of a gene, is a specific variant of a gene that expresses itself more powerfully on its own than any other version of the gene that the individual is carrying—in this example, the recessive—for a variety of reasons.

Now, it mainly refers to patterns of inheritance typically used in combination with a Punnett square, where if a person has two copies of a gene and one is shown to frequently be passed down from one generation to another, it is referred to as dominant.

According to biochemistry, what is happening in this situation is that the genetic variant may, for a variety of reasons, cause a cell to perform a very advantageous or very disadvantageous function that the other version of the gene cannot cover up or counteract.

Therefore, the probability that the next offspring will be pigmented is ¾ as P is the dominant, pigment-producing allele.

Read more about dominant alleles, here

https://brainly.com/question/2717245

#SPJ2

how do humans impact the biodiversity of ecosystems

Answers

Answer:

Humans affect biodiversity by their population numbers, use of land, and their lifestyles, causing damage to habitats for species. Through proper education, and by demanding that governments make decisions to preserve biodiversity, the human population will be able to sustain life on earth longer.

Explanation:

[tex]\texttt{ANSWER:}[/tex]

Human population levels, land usage, and lifestyles all have an impact on biodiversity, causing damage to species' habitats. The human population will be able to maintain life on Earth for longer if adequate knowledge is provided and governments are forced to make decisions that protect biodiversity.

[tex]------------[/tex]

[tex]\texttt{HOPE IT HELPS}[/tex]

[tex]\texttt{HAVE A GREAT DAY!!}[/tex]

Provide the missing word in the
sentence below. Write only the
correct word. Provide the whole
word, the first letter of the missing
word is given as a guide to the
correct word and must be included in
the answer.
In freshwater the blood soittes of a
marine fish would be h... to the water.​

Answers

Answer:

Provide the missing word in the

sentence below. Write only the

correct word. Provide the whole

word, the first letter of the missing

word is given as a guide to the

correct word and must be included in

the answer.

In freshwater the blood soittes of a

marine fish would be h... to the water

Explanation:

Provide the missing word in the

sentence below. Write only the

correct word. Provide the whole

word, the first letter of the missing

word is given as a guide to the

correct word and must be included in

the answer.

In freshwater the blood soittes of a

marine fish would be h... to the water

In freshwater the blood soittes of a marine fish would be heated to the water.

which piece of DNA would have the highest tm one with a cytosine plus guanine comment at t 30% or a cystonie plus guane content of 50% if both are heated under the same conditions

Answers

Answer:

50% G+C will have a higher Tm

Explanation:

The Temperature of Melting (Tm) refers to the temperature at which 50% of double-stranded DNA (dsDNA) is changed to single-standard DNA (ssDNA). In the double helix of DNA, Adenine bases always pair with Thymine bases through two hydrogen bonds, whereas Guanine bases always pair with Cytosine bases through three hydrogen bonds. In consequence, a DNA molecule containing a higher GC content is more stable than another DNA molecule containing a lower GC content. The Tm can be calculated as follows = 2 °C(A + T) + 4 °C(G + C) = °C Tm (this equation is useful for oligonucleotides of 14 to 20 base-length).

The main structural unit of the cell membrane is the protein molecule

Answers

Answer:

The cell membrane consists of a double layer of lipid molecules covered by two layers of proteins. The lipid molecules are parallel to each other and perpendicular to the membrane.

In a species of Asclepias (milkweeds) there are two alleles of a gene involved in the synthesis of cardenolides. One allele results in normal synthesis of these molecules, while the other allele prevents synthesis from occurring. Individuals who are heterozygous for this gene produce intermediate amounts of cardenolides. In a population of Asclepias where both alleles of the cardenolide synthesis allele are present the following phenotypes were observed:
Normal cardenolide concentration: 241
Intermediate cardenolide concentration: 720
No cardenolides: 39
What are the expected phenotypic frequencies in this population?
A.Normal: 0.36; Intermediate: 0.62; No cardenolides: 0.02
B. Normal: 0.36; Intermediate: 0.48; No cardenolides: 0.16
C. Normal: 0.24; Intermediate: 0.48; No cardenolides: 0.04
D. Normal: 0.50; Intermediate: 0.48; No cardenolides: 0.02
E. Normal: 0.24; Intermediate: 0.72; No cardenolides: 0.04

Answers

menenemmek ekekejekkwj wkekejkekw

explain what makes psychology a science.

Answers

Psychology is a science because it follows the empirical method. ... We can directly observe and carefully measure externals such as what a person does, says, and marks down on a psychological test. We cannot directly observe a person's mind (e.g., internal thoughts, emotions).

Try this for yourself. Suppose you want to estimate the number of field mice in a small field. One day you capture 500 mice, mark all of them and release them. The next week you capture 200 mice of which 40 were marked by you yesterday. What is your estimate of the population size?

Answers

Answer:

The approximate size of the field mice population is 2500 individuals.

Explanation:

There are different methods to study population density. There are direct methods and indirect methods. Among these last ones, we might find the "Mark-Recapture technique".

The Mark-Recapture technique assumes that the population is closed,  during the sampling season there is no mortality nor natality, marks in the individual last the whole sampling season, and these do not affect the marked individual or their behavior. These marked individuals are randomly distributed in the population, and all the individuals have the same probability of being sampled.

The method consists of capturing a sample of individuals belonging to the population under study, in this case, the field mice population. After capturing the individuals, the researcher marks them and release them again. The third step is to sample again, capture a new amount of individuals, and count how many of them are marked. These marked individuals belong to the first sample. We can calculate the population size by using the Lincoln Index or any other index.

The Lincolns Index states that to obtain the population size, the research must follow the following procedure:

1) Take an initial sample with n1 number of individuals

2) Mark all the sampled individuals and release them

3) Take a second sample with n2 number of individuals

4) Count the number of marked individuals m

To estimate the total number of individuals in this population, N, the following equation must be considered:

N = (n1 x n2)/m

In the exposed example, this is:

• n1= 500 individuals in the first sample

• n2= 200 individuals in the second sample

• m= 40 individuals in the second sample that were previously marked  

             as they were part of the first sample    

N = (n1 x n2)/m = (500 x 200) / 40 = 2500 individuals

The approximate size of the field mice population is 2500 individuals.  

predators are... a. primary producers b. primary consumers c. secondary consumers

Answers

Answer:

Primary consumers

They eat the majority of prey

When body temperature increases, thermoreceptors are stimulated and send nerve signals to the CNS. The CNS sends motor signals to sweat glands, which attempt to reduce body temperature. This is an example of a __________ reflex.a. organ.b. stretch.c. withdrawal.d. visceral.

Answers

Answer:

d. visceral.

Explanation:

The visceral reflex is one that happens autonomously in the body, aiming to maintain the balance of the body through quick responses to some specific impulses. An example of a visceral reflex is the reduction in body temperature with the release of sweat from the sweat glands.

The visceral reflexes are controlled by the autonomic nervous system, using the sympathetic and parasympathetic nervous system.

2. What is the percentage likelihood that the couple will have a child that has the allele for cystic fibrosis

Answers

Answer:

the answer I got for the question you asked is 75%

Erosion and deposition constantly change Earth’s surface. Erosion carries natural materials like rock and soil from one place to another. Through deposition, these natural materials may be deposited in areas where they build up over time.

Which landform results from the deposition of materials in a valley during volcanic eruptions?

A.sill
B.mud pot
C.caldera
D.lava plateau
(Science)

Answers

D. Lava plateau
They are formed when lava flows from the volcano & build up over time as the molten lava is deposited & cools.

que contiene el condón?

Answers

Answer:

plss translate it in English so i Can easyly answer it.

Explanation:

Thank you.

Which way do the chlorophyll bands move on the chromatography paper?

Answers

Answer:

They move up the chromatography paper.

Because the movement of the chlorophyll bands on the chromatography paper is dependent on the stationary phase and the mobile phase, the pigment separates different colors when moving the top of the paper.

What is the significance of chromatography?

Chromatography is a technique that is used to separate and analyze a mixture of chemical substances and is a powerful tool in various fields such as chemistry and biochemistry. The significance of chromatography is that it allows scientists to identify and quantify individual components in a mixture and these techniques are helpful for the synthesis of drugs and other chemical products.

Hence, because the movement of the chlorophyll bands on the chromatography paper is dependent on the stationary phase and the mobile phase, the pigment separates different colors when moving the top of the paper.

Learn more about chromatography here.

https://brainly.com/question/26491567

#SPJ6

Chordates have a chord in the back for support. In animals with vertebrates, the
chord itself becomes the

Answers

Answer:

1

Explanation:

Why did Mendel use pea plants in his experiments?

Answers

Answer:

For Gregor Mendel, pea plants were fundamental in allowing him to understand the means by which traits are inherited between parent and offspring. He chose pea plants because they were easy to grow, could be bred rapidly, and had several observable characteristics, like petal color and pea color.

Explanation:

#carryonlearning


Chemical rocks are formed by ____ that grow out of a solution.

Answers

Answer:

crystals

Explanation:

In determining soil texture, why is it that the soil samples could all not be moulded into the same number of shapes.

Answers

Answer:

yes

Explanation:

yessnfjejfjdjiwjdjebtrifuejfjr

Which of the following is the best definition of an atom?
O A. A particle within the nucleus that has a neutral charge
B. A negatively charged particle that circles the nucleus
C. The fundamental unit of all matter, both living and nonliving
O D. A positively charged particle within the nucleus
SUBMI

Answers

Answer:

C. The fundamental unit of all matter, both living and nonliving

Several genes in humans in addition to the ABO gene (I ) give rise to recognizable antigens on the surface of red blood cells. The MN and Rh genes are two examples. The Rh locus can contain either a positive or a negative allele, with positive being dominant to negative. M and N are codominant alleles of the MNgene. Suppose a child's blood type is B MN Rh(neg). The mother's blood type is O N Rh(neg). Check all of the blood types below that the father could have. (Assume no involvement of the Bombay phenotype; everyone is HH.)
a) O M Rh(neg)O M Rh(neg)
b) B MN Rh(pos)B MN Rh(pos)
c) A M Rh(pos)A M Rh(pos)
d) O MN Rh(pos)O MN Rh(pos)
e) B MM Rh(pos)B MM Rh(pos)
f) B NN Rh(neg)

Answers

Yes yes yes yes nice

What is silk thread?

Answers

Silk worms are an invasive species in the UK. They cover trees and kill them….. silk comes out of their butt and we call it silk thread. Silk thread is silk spun into thread, or silk worm pooo pooooooo

Why do scientists study seismographs?

Answers

To analyse mineral content from earth's crust

Answer:

To determine the location of an epicenter

Explanation:

A seismogram is a graph output by a seismograph ; a record of the shaking and vibration of the ground. By studying seismograms, scientists learn a lot about Earth's internal structure, like how fast the waves are moving and what type of material the waves are passing through.

Which of the following statement(s) is/are true regarding the adrenal glands' relationship with the autonomic nervous system?

a. The adrenal cortex is an extension of the parasympathetic nervous system.
b. The adrenal medulla secretes norepinephrine when stimulated by the sympathetic nervous system.
c. The adrenal glands are strictly nerve tissue.
d. The parasympathetic division stimulates the adrenal cortex to secrete glucocorticoids.
e. The adrenal medulla is penetrated by the fibers of the sympathetic nervous system.

Answers

Answer:

The true statements regarding the adrenal glands' relationship with the autonomic nervous system are:

a. The adrenal cortex is an extension of the parasympathetic nervous system.

c. The adrenal glands are strictly nerve tissue.

d. The parasympathetic division stimulates the adrenal cortex to secrete glucocorticoids.

e. The adrenal medulla is penetrated by the fibers of the sympathetic nervous system.

Explanation:

The  levels of the central nervous system which play important roles in influencing the autonomic nervous system include cerebral cortex, hypothalamus, brain stem, and spinal cord.  Usually, epinephrine (adrenaline) and norepinephrine are released into the blood stem when stress or a threat occurs.  This alert serves as a warning signal and defense system.  The purpose is to maintain homeostasis.

what precautions should you take to avoid exposure to blood borne pathogens

Answers

Explanation:

The Bloodborne Pathogens standard (29 CFR 1910.1030) and CDC's recommended standard precautions both include personal protective equipment, such as gloves, gowns, masks, eye protection (e.g., goggles), and face shields, to protect workers from exposure to infectious diseases.

Answer:

The Bloodborne Pathogens standard (29 CFR 1910.1030) and CDC's recommended standard precautions both include personal protective equipment, such as gloves, gowns, masks, eye protection (e.g., goggles), and face shields, to protect workers from exposure to infectious diseases.

Other Questions
What is the difference between productive and service oriented professions Explain with examples 1. ______ are costs that do not require a monetary payment A) Accounting costs B) Explicit costs C) All opportunity costs D) Implicit costs2. Joe runs a restaurant. He pays his employees $200,000 per year. His ingredients cost him $50,000 per year. Prior to running his restaurant, Joe was a lawyer earning $150,000 per year. What would economists say is Joes cost of running the restaurant?A) $150,000 B) $200,000 C) $250,000 D) $400,000 At the beginning of a snowstorm, Camden had 10 inches of snow on his lawn. Thesnow then began to fall at a constant rate of 2.5 inches per hour. Assuming no snowwas melting, how much snow would Camden have on his lawn 4 hours after the snowbegan to fall? How much snow would Camden have on his lawn after t hours of snowfalling?Snow on lawn after 4 hours:Show on lawn after t hours: a. In 2018, manufacturing workers in the United States earned average compensation of $21.86 per hour. That same year, manufacturing workers in Mexico earned average compensation of $3.20 per hour. U.S. manufacturers can possibly compete if multiple choice 1 the product in the United States is specialized. technology is cheaper in the United States. U.S. workers are more productive. some production is done in low-wage countries. b. All manufacturing is not done in Mexico and other low-wage countries because multiple choice 2 there is not enough foreign labor. of trade barriers. shipping costs are too high. consumers want domestically produced products. 1. The company will spend more money on the ------- of environment-friendly vehicles. (A)develops (B) developed (C) develop (D) development find the greatest common factor of the following monomials 49a4b3 21a4b5 You write (5-2)n to represent the phrase 2 less than 5 divided by a number n. Your friend writes (5n)-2. Are these both reasonable interpretations? Can verbal descriptions lack precision? Explain i have the answer, 2-(3/x+2)i got this from my calculator, however i need another line of working and im unsure of the process used to get there What are the root(s) of the quadratic equation whose related function is graphed below? A person has power of 550 watt. What does it mean? They (know) the result in a couple of day( future indefinite form) If the nth term of A.P. are 7, 12, 17, 22.....is equal to the nth term of another A.P. 27, 30, 33, 36. Find the value of n? The residual income valuation model is a rigorous and straightforward valuation approach, but the analyst should be aware of all of the following implementation issues that will hinder its ability to measure firm value correctly except: _________a. common stock transactionsb. portions of net income attributable to equity claimants other than common shareholdersc. dirty surplus accounting itemsd. positive book value of equity I need help with the incorrect ones please A circular parallel-plate capacitor whose plates have a radius of 25 cm is being charged with a current of 1.3 A. What is the magnetic field 11 cm from the center of the plates what are the four elements of a text Which details of Beatty's account of firemen make it credible/incredible? Is his account a history or simply a fable meant to influence Montag's behavior? draw two intersecting line segment AB and CD intersecting O. Measure the size of each pair of vertically opposite angles. Verify each pair of vertically opposite angles are equal. Which of the following behaviors would best describe someone who is listening and paying attention? a) Leaning toward the speaker O b) Interrupting the speaker to share their opinion c) Avoiding eye contact d) Asking questions to make sure they understand what's being said What is the application of Gene Editing in Primary T Cells?Creative Biogene CRISPR/Cas9 Platform provides comprehensive gene editing services for various cell lines, especially for primary T cells. With years of experience, our talented scientists have established an efficient assay to edit genome in T cells by CRISPR/Cas9. At CRISPR/Cas9 PlatformCB, we offer a gene knockout service for customer as well as isolation of primary T cell service. Based on our excellent platform, our staff are focusing on supplying the best services and products.https://www.creative-biogene.com/crispr-cas9/service/gene-editing-in-primary-t-cells.html