The CRISPR/Cas9 system can cleave genomic DNA at sequences other than the desired target, a phenomenon referred to as off-target cleavage. To avoid off-target cleavage, sites targeted for mutation should appear only once in the genome. What is the minimum length of the part of an sgRNA molecule that binds DNA whose base pair sequence would be expected to be unique within the fruit fly genome
Answers
Answer:
correct answer: 14
Explanation:
the question is incomplete, so a graph with the complete questionnaire is attached and the requested question is answered
We will start from the exercise requesting the minimum length that a sequence forms with more than 1.4x10ˆ8 permutations
for this we deduce that two base pairs can have:
4 * 4 = 16 permutations
we deduce that 3 base pairs can have:
4 * 4 * 4 = 64 permutations
Thus we multiply successively until we reach that 14 base pairs can obtain: 2.68x10ˆ8 permutations that are random
Thus, the 14bp sequence will be found only once in the genome of the fruit fly.
Related Questions
what is a range of the lowest temperatures found at the top of the tropopause
Answers
Answer:
62°F (17°C) to -60°F (-51°C) at the tropopause.
What is the comparison and the differences between the structures of the stomach and the pancreas?
Answers
Answer:
Explanation:
The chyme produced from the stomach is highly acidic in nature; the pancreatic juices contain high levels of bicarbonate, an alkali that neutralizes the acidic chyme. Additionally, the pancreatic juices contain a large variety of enzymes that are required for the digestion of protein and carbohydrates.
Where is new ocean floor formed?
Answers
Answer: Mid ocean ridges
Explanation:
Earth.
Answer:
Hey! New ocean floor is formed at mid-ocean ridges.
Explanation:
. Your lab technician was to determine the ash content of buttermilk by conventional dry ashing. The technician weighed 5 g of liquid buttermilk into one weighed platinum crucible, immediately put the crucible into the muffle furnace using a pair of all stainless steel tongs, and ashed the sample for 48 h at 800 °C. The crucible was removed from the muffle furnace and set on a rack in the open until it was cool enough to reweigh. (4 marks)
a. What errors were made in the preceding method (there were several)?
b. What were the likely results of these errors?
Answers
Answer:
Ashing process is done to evaluate the ash content present in petroleum or other proucts, so that they can be used for certain applications
The two major types of ashing process that includes dry ashing and wet ashing and can be performed using microwave systems or conventional means.
a. errors made in the preceding method while determining the ash content of buttermilk by conventional dry ashing are as following:
Buttermilk should not immediatly put into muffle immediatley, it should be boiled first to avoid splatter.Temperature should be between 500 to 600 degree celcius at high temperature teh ash content can be loss.The sample should be ashed for 12-18 hrs , more than this can damage the required ash content.platinum tip should be used in place of stainless steel because platinum has a lower concomitant tip impedance than stainless stell, so less reactive to ash.The ash should put in dessicator and not allow to kept in open because ash can react with the humidity in teh atmosphere.b. The result that occur due to above errors such as the temperature, time, tongs and leaving ash in open will manipulate the final ash content and its application can be harmful for humans and for other purpose if it will be used.
18. Some groups of organisms work together to stay safe or to take down prey. What do we
call this?
Answers
Answer: Mutualistic.
Explanation:
which trait does the fly agaric and the palm have in common
Answers
Help with the picture
Answers
When a plant generates food through photosynthesis, this food
A.
is always used as soon as it is converted into a form the plant can use.
OB
can be used immediately or stored for later use.
C.
can cause the plant to gain excess layers of fat.
OD
is stored for a short period of time, usually about three weeks,
Answers
Answer:
B:because plant will make the food,store it,or eat it.usually the plant will stored the food for the winter,because of the soil being frozen and barely any sunlight,it will make it hard for the plant to make more food during photosynthesis,sometimes the plants will die but come back when it is springtime.Once food is made it is used or saved for later use for energy.
Answer:
can be used immediately or stored for later use.
Explanation:
Energy generated through photosynthesis can be used immediately or stored for later use depending on the needs of the plant in its environment.
Sometimes, a plant needs to immediately put this energy into growing new branches or leaves. Other times, such as when a cold winter is approaching, it might be better for the plant to store the energy for later use
Drag the tiles to the correct boxes to complete the pairs.
Match the whorl fingerprint subclasses to their descriptions.
Answers
Answer:
A loop with a whorl: central pocket loop
Two loops with an s pattern: double loop
irregular shaped whorl: accidental loop
series of loops with a common center: plain loop
Explanation:
Ok I believe its: Loop with a whorl- plain, 2 loops with a S pattern- double loop, Series of loops with a common center- central pocket loop, and the Irregular-shaped whorl is a accidental loop. If I helped please mark brainliest!! :)
The codon, CAG, is being carried by a mRNA strand into the
cytoplasm. By using the Codon Chart, what amino acid will it code
for?
Answers
e:
Scientists think the earth is approximately how old?
2 billion years
3 billion years
for 4 12 billion years
612 billion years
Scientists think the earth is approximately how old
Answers
Answer:
4 billion
Explanation:
Answer:
4 billion
Explanation:
4 billion
How is food moved through the gut?
a. By peristalsis c. By waves of liquid
b. By gravity
d. Movement
Answers
Explanation:
a. By peristalsis
..................
which species is the banana most closely related to?
lemon
onion
radish
seaweed
Answers
Answer:
onion
Explanation:
"Onions are most closely related to a) banana. Onions are part of the Amaryllidaceae family and bananas are part of the Musaceae family. They are both monocots, meaning the have one cotyledon, parallel leaf veins, and flower petals in multiples of three, among other distinctive characteristics. "
Answer:
Onions are most closely related to a) banana. Onions are part of the Amaryllidaceae family and bananas are part of the Musaceae family. They are both mono cots, meaning the have one cotyledon, parallel leaf veins, and flower petals in multiples of three, among other distinctive characteristics.
Hope this helps!
Please give as brainliest answer!!
Thanks!
Explanation:
The outermost cells of the skin die and slough off because
Answers
Answer:
Explanation:
The keratinocytes: cells found in the outermost layer of the epidermis that produces fibrous protein which gives hair, nails, and skin their hardness and water-resistant properties dies as a result of their distance from the rich blood supply the cells of the next layer lie on top off. Due to less nutrients and oxygen, these cells die as they are pushed towards the surface of our skin.
If the bubbles given off by the plants were collected and tested, the gas present would MOST likely be
(A) oxygen
(B) hydrogen
(C) nitrogen
(D) carbon dioxide
Answers
Answer: D
Explanation: To prove that oxygen is a by-product of photosynthesis, you can set up an experiment with an aquatic plant and capture the gas it emits in response to photosynthesis.
75 POINTS!!! One reason male peacocks spread their tail feathers is to attract a mate. How does choosing a male with a bright, full tail increase a peahen’s chance of producing healthy peachicks?
Answers
Answer:
They have greater health conditions
Explanation:
if they are bright, they are healthy
Answer:
Typically, if a peacock has a bright tail with many feathers and eyespots, a female will choose him. These tail features indicate that the peacock may be healthier than other males and, therefore, produce healthier peachicks.
Explanation:
This is the sample response.
The immunity rate is the ____________ variable. The number of people affected by the disease is the ___________ variable.
Answers
Answer: 1. independent , 2 . dependent
The immunity rate is an independent variable. The number of people affected by the disease is the dependent variable.
What is immune system?
The immune system protects our body from foreign invaders like bacteria, viruses, and fungi, and other toxins.
In general 2 types of the immune system observed in human body.
The innate immune system rapid inheritable response system consist major organs like skin, the eye's cornea, and the mucous membrane that lines the respiratory, gastrointestinal, and genitourinary tracts.
Secondly, the acquired immune system work with the innate system to make a special proteins named antibodies to protect our body from a specific invader.
Human immune system show variation in their production of immune components like cytokines play critical roles and it vary from person to person.
Hence, The immunity rate is an independent variable. The number of people affected by the disease is the dependent variable.
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new biology question pls help
Answers
Answer:
it should be natural selection but im not too sure...
Explanation:
Answer:
The theory of natural selection was explored by 19th-century naturalist Charles Darwin. Natural selection explains how genetic traits of a species may change over time. This may lead to speciation, the formation of a distinct new species.
Explanation:
g What basic characteristics do we generally expect to find in all things that qualify as being alive? Question 1 options: 1) an ability to: reason, make polymers and repair damage. 2) an ability to: reproduce, respond to stimuli and repair damage. 3) an ability to: avoid pain, move from one place to another and evolve. 4) an ability to: capture energy from sun light, move from one place to another and reshape its environment. 5) an ability to be more ordered than their surroundings, respond to stimuli, and capture energy from sunlight.
Answers
Answer:
Number 2: an ability to: reproduce, respond to stimuli and repair damage.
Explanation:
Some basic characteristics of all living things in general which they all have in common include:
The ability to reproduce (reproduction). All living things reproduce...
Ability to respond to stimuli (irritability)
Ability to repair damage: this is involved with cells in the organisms and to stay alive all organismsin general areade up of cells which has the ability to be repaired if damaged.
The equation AU = Q - W describes which one of the following?
O A. Second law of thermodynamics
O B. Efficiency of a heat engine
O C. Work done by a gas
O D. First law of thermodynamics
SUBMI
Answers
Answer:
D. First law of thermodynamics
Explanation:
The first law of thermodynamics refers that energy can be tranferred or changed form one form to another but it can neither be destroyed nor be created. hence, it is also known as law of conservation of energy.
The equation ΔU = Q − W describes the first law of thermodynamics.
Where
U is the internl energy of the system. ΔU is the change in U. Q refers to the total heat supplied into the system (include both addition and release of energy in the system). W is the net work done by the system.Hence, the correct option is D.
Answer:
Option D, First law of thermodynamics
Explanation:
The first law of thermodynamics states the following -
[tex]dU = Q-W[/tex]
where dU represents the Heat added to the System
Q represents the Change in Internal Energy OR net heat transfer OR sum of all heat transferred into and out of the system
W represents the Work done on the system
The first law of thermodynamics is based on the application of the conservation of energy principle to heat and thermodynamic.
hence, option D is correct
How long does it take for Earth to make one rotation on its axis? A. 1 day B. 1 month C. 1 hour D. 1 year
Answers
Answer- A
Explain- Because in order for the Earth to completly turn around, it takes around 23.8 hrs, which is about 1 day.
Answer:
A. 1 day
Explanation:
It only takes 23 hours, 56 minutes and 4.0916 seconds for the Earth to turn once its axis.
2.In what way is the plant’s vascular system like ours?
3.How have scientist classified all plants?
4.Give 2 examples of non-vascular plants. (This will take a little extra research.)
5.List the 2 major vascular tissues of a plant and tell what they do.
1.
2.
6.List the reactants and products of photosynthesis?
Reactants:
Products:
7.Both plant and animal cells have organelles. What are organelles?
8.Which organelle is responsible for photosynthesis?
9.Why is it incorrect to say plants “breathe” Carbon dioxide (CO2)?
10.Where would you expect to find plant cell with a high number of chloroplast?
11.What are the stomata and why are they important to the plant?
12.What is the job of the guard cells?
13.The venus fly trap or the pitcher plant are still considered plants. Why do they “eat” insects?
14 Your friend thinks mistletoe is normal plant. How could you explain why it is NOT considered a “normal” plant?
Answers
Answer:
2.The human circular system does pretty much the
same thing as a plant but the plant can also reproduce oxygen. The
human needs air so does a plant to live and make more air. The
human needs food so does a plant to stay healthy. The human needs
to drink water to live too so does the plant to stay alive
otherwise it will rot and fall apart.
Explanation:
Insulin is a hormone that travels through the blood and helps regulate blood sugar levels. Which best describes how insulin travels in the blood?
in the plasma
in the platelets
attached to the white blood cells
attached to the red blood cells
Answers
Answer:
A:in the plasma
Explanation:
Insulin regulates blood sugar levels and it travels in the plasma. Therefore option A is correct.
What is insulin?Insulin is a hormone that is released by β-cells of the pancreas. It controls the amount of glucose in our bodies. It converts glucose into glycogen and then this glycogen is stored in the liver, and whenever the glucose level reduces in the blood, then this stored glycogen is converted into glucose. It also helps in breaking fat and protein for energy.
Insulin is a very important hormone for the regulation of glucose in the blood. A deficiency of insulin occurs when the immune system attacks islets and it causes diabetes, in this disease blood sugar level increases
.
Insulin flows in the blood through plasma. Therefore, option A is correct.
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The inner protective layer of the viral genetic information
Answers
Answer:
the capsid is the inner protective layer of the viral genetic information(rna)
Which resource is found in large quantities in China and is also imported into China from other countries?
tungsten
pearls
coal
timber
Answers
Answer:
Coal
Explanation:
China fulfills its demand for coal by purchasing it from regional neighbors. In 2017, its coal imports mainly came from Australia (79.9 million tons), Indonesia (35.2 million tons), Mongolia (33.5 million tons), and Russia (25.3 million tons).
Hope this helps
Have a great day!
Recessive sex-linked traits such as color blindness appear more frequently in
males because
A. males inherit only one sex chromosome.
B. males inherit two copies of every sex-linked allele.
C. sex-linked alleles are carried only on Y chromosomes.
D. males inherit only one sex-linked allele.
Answers
Explanation: In humans, recessive sex-linked traits generally occur on the X chromosome. Since males are (XY), they will inherit only one sex-linked allele on their X chromosome.
Hope that helps! :)
What species does the mosquito belong to?
Answers
Answer:
Insects
Explanation:
Answer:
flies (order Diptera). Within Diptera they constitute the family Culicidae
Explanation:
Which of the following statements about oxidative phosphorylation by ATP synthase is FALSE? A) The electrochemical gradient across the inner mitochondrial membrane generated by electron flow is used to synthesize ATP. B) Uncouplers dissipate the electrochemical gradient but electron transport continues without ATP production. C) Inhibitors disrupt electron flow as well as ATP synthesis. D) A proton gradient alone without a corresponding energy input is not sufficient to drive the synthesis of ATP. E) ATP synthesis occurs via simultaneous conformational changes in protein subunits containing catalytic sites.
Answers
Answer
D)A proton gradient alone without a corresponding energy input is not sufficient to drive the synthesis of ATP
Explanation:
Oxidative phosphorylation reffers to metabolic pathway whereby enzyme is used by the cell to achieve nutrient oxidation, so that chemical energy of molecular oxygen is released as a result of this, adenosine triphosphate (ATP) is manufactured. oxidative phosphorylation is seen in all organisms that are aerobic in nature and it occur in the mitochondria of all eukaryotic organisms. There is electron transfer during oxidative phosphorylation, the electrons moves from the donor to the receptor and this is known as redox reaction, as a result of this energy is released, and this energy is used for formation of ATP.
Therefore,among all the options, A proton gradient alone without a corresponding energy input is not sufficient to drive the synthesis of ATP is the false statement.
Meiosis reduces the chromosome number by
Answers
Answer:
i believe it's half and produces four gamete cells
Explanation:
Select all the following that describe isotopes of an element
_ Different numbers of neutrons
_ Different mass numbers
_ Different atomic numbers
_ Different numbers of protons
Answers
Answer: different number of neutrons and different mass numbers
Explanation:
The statements that describe isotopes of an element are:
Different numbers of neutrons.Different mass numbers.What is an isotope?An isotope is defined as varieties of atoms that have the same atomic number and therefore constitute the same element even though they have different mass numbers.
Characteristics of an isotope of an elementAtoms that are isotopes of each other have the same number of protons in the nucleus.They are found in the same place within the periodic table.Therefore, we can conclude that the isotopes of an element are atoms with the same atomic number but different mass numbers.
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