Answer:
25 mM Tris HCl and 0.1% w/v SDS
Explanation:
A 10X solution is ten times more concentrated than a 1X solution. The stock solution is generally more concentrated (10X) and for its use, a dilution is required. Thus, to prepare a buffer 1X from a 10X buffer, you have to perform a dilution in a factor of 10 (1 volume of 10X solution is taken and mixed with 9 volumes of water). In consequence, all the concentrations of the components are diluted 10 times. To calculate the final concentration of each component in the 1X solution, we simply divide the concentration into 10:
(250 mM Tris HCl)/10 = 25 mM Tris HCl
(1.92 M glycine)/10 = 0.192 M glycine
(1% w/v SDS)/10 = 0.1% w/v SDS
Therefore the final concentrations of Tris and SDS are 25 mM and 0.1% w/v, respectively.
A brown dye has a percent composition of 62.41% C, 5.24% H, and 32.36% N by mass with a molar mass of 346.40 g/mol. Determine the molecular formula of the dye
The molecular formula : C₁₈H₁₈N₈
Further explanationGiven
62.41% C, 5.24% H, and 32.36% N
Required
The molecular formula
Solution
mol ratio
C : 62.41/12.0096 = 5.1967
H : 5.24/1.00784 = 5.1992
N : 32.36/14.0067 = 2.310
Divide by 2.310(smallest)
C : 5.1967/2.31=2.25
H : 5.1992/2.31 = 2.25
N : 2.31/2.31 = 1
Multiplied by 4
C : H : N = 9 : 9 : 4
The empirical formula : C₉H₉N₄
(C₉H₉N₄)n=346.40 g/mol
(12.0096 x 9 + 1.00784 x 9 + 14.0067 x 4)n=346.4
(108.0864+9.07056+56.0268)n=346.4
(173.184)n=346.4
n=2
The molecular formula : C₁₈H₁₈N₈
How many orbitals in an atom can have each of the following designations:
(a) 1s;
(b) 4d;
(c) 3p;
(d) n=3?
Answer:
(a) 1s; has one orbital
(b) 4d; has five orbitals
(c) 3p; has three orbitals
(d) n=3 has nine orbitals
Explanation:
Electrons in an atom are always in constant motion, making it hard to predict there exact position. However, the most probable locations electrons can be be found are described with the terms shells, subshells and orbitals. A shell contains subshells and orbitals are found within subshells. The shells are given names such as K, L, M, N, which correspond to the principal quantum numbers, n = 1, 2, 3, and 4 respectively. There are 4 major types of subshells that can be found in a shell. They are named as s, p, d, f. Each subshell is composed of several orbitals.
a. 1s; the s subshell has only one orbital. Therefore, the 1s subshell has one orbital
b. 4d; the d subshell has five orbitals. Therefore, the 4d subshell has five orbitals
c. 3p; the p subshell has three orbitals. Therefore, the 3d subshell has three orbitals
d. n = 3; the shell with n = 3 has the following subshells, 3s, 3p, 3d.the number of orbitals will be 1 + 3 + 5 = 9 orbitals. Therefore, the number of orbitals in n = 3 is nine orbitals
What volume (in L) of water vapor will be
produced from the reaction of 24.65 L of oxygen?
2C2H6(9) + 702(9) — 4CO2(g) + 6H20(9)
Enter
Answer:
21.13 L
Explanation:
Step 1: Write the balanced equation
2 C₂H₆(g) + 7 O₂(g) ⇒ 4 CO₂(g) + 6 H₂O(g)
Step 2: Determine the appropriate volume ratio
Since all the gases are in the same container at the same temperature and pressure, the volume ratio is equal to the molar ratio, because the volume depends on the number of moles. The volume ratio of O₂(g) to H₂O(g) is 7:6.
Step 3: Determine the volume of H₂O produced from 24.65 L of O₂
24.65 L O₂ × 6 L H₂O/7 L O₂ = 21.13 L H₂O
A change of state is a(n)
process.
A. irreversible
B. reversible
Answer:
Changes of states are reversible, you can go from a solid to liquid and liquid to solid.Answer:
Reversible
Explanation:
Changes of state are physical changes in matter. Common changes of the state include melting, freezing, sublimation, deposition, condensation, and vaporization.
Classify each of the following compounds as a strong acid, weak acid, strong base, or weak base, and write the Ka expression for any weak acid or weak base:
1. [ Select ] ["strong base", "weak base", "strong acid", "weak acid"] LiOH
2. [ Select ] ["weak acid", "strong acid", "strong base", "weak base"] HF
3. [ Select ] ["strong acid", "weak acid", "strong base", "weak base"] HCl
4. [ Select ] ["weak base", "strong base", "weak acid", "strong acid"] NH3
Ka expression: [ Select ] ["[H+][F-] / [HF]", "[Li+][OH-]/ [LiOH]", "[H+][Cl-} / [HCl]", "[NH4+] / [NH3]", "[HF] / [H+][F-}", "[LiOH] / [Li+][OH-]", "[HCl] / [H+][Cl-}", "none"]
Calculate the concentration of OHLaTeX: -? in a solution that has a concentration of H+ = 7 x 10LaTeX: -?6 M at 25°C. Multiply the answer you get by 1010 and enter that into the field to 2 decimal places.
Answer:
See explanation below
Explanation:
There are several ways to know if an acid or base is strong. One method is calculating the pH. If the pH is really low, is a strong acid, and if it's really high is a strong base.
However we do not have a pH value here.
The other method is using bronsted - lowry theory. If an acid is strong, then his conjugate base is weak. Same thing with the bases.
Now, Looking at the 4 compounds, we can say that only two of them is weak and the other two are strong compounds. Let's see:
LiOH ---> Strong. If you try to dissociate :
LiOH ------> Li⁺ + OH⁻ The Li⁺ is a weak conjugate acid.
HF -----> Weak
HF --------> H⁺ + F⁻ The Fluorine is a relatively strong conjugate base.
HCl -----> Strong
This is actually one of the strongest acid.
NH₃ ------> Weak
Now writting the Ka and Kb expressions:
Ka = [H⁺] [F⁻] / [HF]
Kb = [NH₄⁺] [OH⁻] / [NH₃]
Finally, to calculate the [OH⁻] we need to use the following expression:
Kw = [H⁻] [OH⁻]
Solving for [OH⁻] we have:
[OH⁻] = Kw / [H⁺]
Remember that the value of Kw is 1x10⁻¹⁴. So replacing:
[OH⁻] = 1x10⁻¹⁴ / 7x10⁻⁶
[OH⁻] = 1.43x10⁻⁹ M
And now, multiplying by 10¹⁰ we have:
[OH⁻] = 1.429x10⁻⁹ * 1x10¹⁰
[OH⁻] = 14.29Hope this helps
Strong acids and bases are those which completely ionized in body fluid, and weak acids and bases are those who does not completely ionized in body fluid.
Ka expression is used to differentiate between strong and weak acids.
Which are strong acids and base and weak acids and bases?LiOH - strong baseHF - weak acidHCl - strong acidNH3 - weak baseWhat are the Ka expression of the following?Weak acid – HF[tex]\bold{\dfrac{[H+][F-]}{[HF]}}[/tex]
Weak base – NH3[tex]\bold{\dfrac{[NH_4^+] [OH^-]}{[NH_3]} }[/tex]
Calculate the concentration of OH?Given, [tex]\bold{ [H^+]=1\times10^-^6\; at \;25^oC}[/tex]
We know, [tex]\bold{ [H^+]\times[OH^-]=1\times10^-^6\; at \;25^oC}[/tex]
[tex]\bold{[OH^-]=\dfrac{1\times10^-^1^4}{6.2\times10^-^6} = 1.43\times10^-^9}[/tex]
Now, multiplying the value by [tex]10^1^0[/tex]
[tex]\bold{( 1.429\times10^-^9) \times 1\times10^1^0= 14.29}[/tex]
Thus, the value is 14.29.
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Why is this image a good model for the Law of Conservation of Mass?
Answer:
The Law of Conservation of Mass states that mass is neither created nor destroyed in chemical reactions. Since the number and type of atoms in the reactant side of the chemical equation are the same as on the product side, the Law of Conservation of Mass has been demonstrated.
Explanation:
In the answer.
The two main postulates that was given by Antoine Lavoisier are, oxygen play an important role in combustion and the other is mass of the reactant and product is conserved. Therefore, the given image is a good model for the Law of Conservation of Mass.
What is law of conservation of mass?According to Law of conservation of mass, mass can neither be created nor be destroyed. Mass can only be transformed from one form to another. The law of conservation of mass was given by Antoine Lavoisier.
Every reaction in nature follow the law given by Antoine Lavoisier that is mass is always conserved. Another postulated that was given by Antoine Lavoisier is the role of oxygen in combustion reaction. Combustion can not happen without presence of oxygen.
The image is a good model for the Law of Conservation of Mass as number and type of atoms on the reactant side and product side are the same.
Therefore, the given image is a good model for the Law of Conservation of Mass.
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In a space shuttle, the Carbon dioxide, CO2 that the crew exhales is removed from the air by a reaction within canisters of Lithium Hydroxide, LiOH. The LiOH is only 85% efficient. On average, each astronaut exhales around 20.0 mol of CO2 every day. What volume of water is produced when the CO2 reacts with the excess LiOH
Answer:
What volume of water is produced when the CO2 reacts with the excess LiOH
X = 360 mL H2O
Explanation:
CO2 (g) + 2 LiOH(s) ⇒ Li2CO3 (aq) + H2O(l)
20.0 mol excess x g
X = 360 mL H2O
x mL H20 = 20.0 mol CO2 (1 mol H2O /1 mol CO2)(18 g H2O/1 mol H2O)
(1 mL H2O /1 g H2O)
X = 360 mL H2O
When measuring the volume of a liquid, how would sample size (e.g., using a 10 mL graduated cylinder vs. a 100 mL graduated cylinder to measure out 70 mL of a liquid) affect the absolute error and percentage error in the measured values of mass and volume and therefore the density
Answer:
Explanation:
From the given information:
The accuracy depends on the internal diameter of the cylinder. The cylinder with the least internal diameter is obviously more precise.
Let's assume 1% is the error of measurement.
Then, to measure 70 mL from 10 mL cylinder
The error = [tex]10 \times \dfrac{1}{100} \times 7[/tex]
= 0.7 mL
However; for a 100 mL cylinder, the error = 1 mL
Now,
The total volume for 10 mL = (70 + 0.7) = 70.7 mL
The total volume for 100 mL = (70 + 1 ) = 71 mL
Suppose the density (d) is same for both
Then;
the mass of 10 mL = ( d × 70.7) g
the mass pf 100 mL = (d × 71) g
Thus, the mass of 100 mL is greater than that of 10 mL.
HELLOSOEOODORODODODORRLKRKLRLRLF
Answer:
I'd say maybe Dangerous? I hope this helps
Answer:
golden is sometimes referred to as shiny and beatitful
Explanation:
Write a description of how you know a chemical reaction is occurring.
ANSWER FAST FIRST PERSON GETS BRAINLIEST
Answer:
See explanation
Explanation:
Chemical reactions are also referred to as chemical change. A chemical change often leads to the formation of new substances and is not easily reversible.
A chemical reaction may be accompanied by the emission of heat and light, formation of a precipitate, evolution of gas, or a color change.
These observable physical effects may tell us weather a chemical reaction has taken place or not so we have to observe the system closely for any of these effects stated above.
which type of bond involves 2 different metals?
A. ionic
B. Covalent
C.Metallic
D. Bonding would not occur
Answer:
iconic bond is the answer
I hope it helps you ✌
Name the intermolecular force that corresponds to: an attraction between a partially positive region in one molecule and a partially negative region in another molecule. an attraction between two temporarily polarized molecules. an attraction between a negatively charged particle and a partially positive region in a molecule. an attraction between a partially positive hydrogen atom in a molecule and a partially negative and highly electronegative atom on another molecule.
Answer:
an attraction between a partially positive region in one molecule and a partially negative region in another molecule....dipole-dipole interaction
an attraction between two temporarily polarized molecules...dispersion forces
an attraction between a negatively charged particle and a partially positive region in a molecule....ion dipole interaction
an attraction between a partially positive hydrogen atom in a molecule and a partially negative and highly electronegative atom on another molecule....Hydrogen bonding
Explanation:
Some molecules have permanent dipole. As a result of this, the positive part of one molecule may attract the negative part of the other molecule leading to dipole-dipole interaction.
Dispersion forces occur in all molecules and is as a result of temporary polarization of a molecule due to instantaneous dipole–induced dipole attractions.
If a charged particle is attracted by a dipole in a molecule, we call it ion-dipole interaction.
When hydrogen is bonded to a highly electronegative element, the positive end of the dipole is on hydrogen while the negative end of the dipole is on the electronegative element. Molecular associations often result from this permanent dipole and is called hydrogen bonding, e.g, HF.
Answer the given question: use Chapter 1 of notebook
How many formula units are there in 3.20x 102 moles of LICI? (2pts.)
Conversion factor: 1 mole LICI=
_formula of LICI
Students, draw anywhere on this slide!
Answer:
1.93 x 10²⁶ formula units
Explanation:
Given parameters:
Number of moles of LiCl = 3.2 x 10² moles = 320moles
Unknown:
Number of formula units in this compound = ?
Solution:
The mole is used a reference to determine the number of substances matters contain.
A mole contains the Avogadro's number of particles.
So;
1 mole of a substance contains 6.02 x 10²³ formula units;
320 moles of LiCl will contain 320 x 6.02 x 10²³ = 1.93 x 10²⁶ formula units
What is the difference between the grassland and savanna biomes?
A)Grassland biomes are closer to the equator and receive more rain.
B)Savanna biomes are closer to the equator and receive more rain.
C)Grassland biomes contain mostly grasses.
D)Savanna biomes contain mostly grasses.
Answer:
C)Grassland biomes contain mostly grasses.
Explanation:
The main differences between a savanna biome and a grassland is that grasslands biomes contain mostly grasses.
Savanna are made up of both grasses and shrubs.
Savanna are a mix of grasslands with some scattered trees in the area. Grasslands are open grass fields. They do not contain much trees as such. The savanna and grasslands are renowned for their fauna diversity of beasts.A reaction between liquid reactants takes place at in a sealed, evacuated vessel with a measured volume of . Measurements show that the reaction produced of sulfur hexafluoride gas. Calculate the pressure of sulfur hexafluoride gas in the reaction vessel after the reaction. You may ignore the volume of the liquid reactants. Round your answer to significant digits.
Answer:
0.41 atm
Explanation:
A reaction between liquid reactants takes place at 10.0 °C in a sealed, evacuated vessel with a measured volume of 5.0 L. Measurements show that the reaction produced 13. g of sulfur hexafluoride gas. Calculate the pressure of sulfur hexafluoride gas in the reaction vessel after the reaction. You may ignore the volume of the liquid reactants. Round your answer to 2 significant digits.
Step 1: Given data
Temperature (T): 10.0 °CVolume of the vessel (V): 5.0 LMass of sulfur hexafluoride gas (m): 13. gStep 2: Convert "T" to Kelvin
We will use the following expression.
K = °C + 273.15
K = 10.0 °C + 273.15 = 283.2 K
Step 3: Calculate the moles (n) of SF₆
The molar mass of SF₆ is 146.06 g/mol.
13. g × 1 mol/146.06 g = 0.089 mol
Step 4: Calculate the pressure (P) of SF₆
We will use the ideal gas equation.
P × V = n × R × T
P = n × R × T/V
P = 0.089 mol × (0.0821 atm.L/mol.K) × 283.2 K/5.0 L = 0.41 atm
what state of matter travels in straight lines
Answer:
light
Explanation:
light is plasma, which is a state of matter
My labmate discovered a new compound and wanted to try to determine what it could be. We noticed that when mixed with an acid, it formed a white solid. What type of chemical reaction did the new chemical just undergo?
Answer:
it went under the process known as crystalization
Explanation:
am a chemist
first answer will get brainliest
Answer:
Incorrect
Explanation:
Mole ratios are derived from the coefficients in front of the number, N2O does not have a 2 in front of it, the real mole ratio would be 4/1
Answer:
Correct
Explanation:
I had the same problem and I put correct, and it was correct.
You want to compare the malleability of
two metals. Plan an investigation that would allow you to determine
which metal is more malleable .
The temperature and the strength of the metallic link are the two most crucial variables that can impact how malleable a metal or alloy is.
What is metal ?A metal is a substance that has a shiny look when freshly processed, polished, or shattered, and conducts electricity and heat rather effectively. Generally speaking, metals are malleable and ductile.
The amount of pressure that a metal can sustain without breaking can be used to gauge its malleability. Varied metals have different degrees of malleability because of variations in their crystal structures.
The temperature of the metal and the strength of the metallic connection are the two parameters that define how malleable a metal or metal alloy will be.
Thus, The kind of the metallic connection can have a significant impact on how easily metal atoms can rearrange themselves.
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How many moles of sodium are present in 17 g of Na?
What is the number of moles in 60g of Na2SO4?
How many moles are there in 93.5g of CO2?
How many moles are there in 25.6g of Sodium Nitrate?
Determine the number of particles in .75 mol of calcium hydroxide
Answer:
1. 0.74mol
2. 0.42mol
3. 2.125mol
4. 0.301mol
5. 4.52 × 10^23 particles
Explanation:
Number of moles (n) in a substance can be found using the formula:
mole (n) = mass/molar mass
Using this formula, the following moles are calculated:
1. Molar of Na = 23g/mol
mole = 17/23
mole = 0.74mol
2. Molar mass of Na2SO4 = 23(2) + 32 + 16(4)
= 46 + 32 + 64
= 142g/mol
Mole = 60/142
mole = 0.42mol
3. Molar mass of CO2 = 12 + 16(2)
= 12 + 32
= 44g/mol
mole = 93.5/44
mole = 2.125mol
4. Molar mass of sodium nitrate (NaNO3) = 23 + 14 + 16(3)
= 23 + 14 + 48
= 85g/mol
mole = 25.6/85
mole = 0.301mol
5. Number of particles in one mole of a substance is 6.022 × 10^23 particles. Hence, in 0.75mol of calcium hydroxide (Ca(OH)2, there will be;
0.75mol × 6.02 × 10^23
= 4.515 × 10^23
= 4.52 × 10^23 particles
A 1.0 mol sample of he(g) at 25 is mixed with a 1.0 mol sample of Xe(g) at 50 C. What would be the changes in average kineeteic energy and the average speed of the Xe atoms that will occur as the mixture approaches thermal equilibrium?
Answer:
Explanation:
The average kinetic energy for an ideal gas is directly proportional to the temperature. The average kinetic energy of the gas is a measure of the temperature of the gas molecule
Also, the average speed is usually proportional to the square root of temperature.
Similarly, there is a noticeable increase in K.E and speed in regard to temperature but sometimes it is not usually proportional.
However, provided that there is more temperature in Xe as compared to He, then after the mixture of both takes place at equilibrium; the temperature tends to fluctuate between (25 - 50)°C
Thus, since there is a decrease in temperature in Xe, both the average kinetic energy as well as the speed too will also decrease.
Vinegar is insoluble in vegatable oil. Does this mean that vinegar is a totally insoluble substance?
Answer:
No
Explanation:
This does not mean that vinegar is insoluble totally. In fact, vinegar is soluble in water because water is a polar solvent.
For a substance to be soluble in another, it must obey the rule of solubility.
The rule states that "like dissolves like"
It implies that polar solvent will only dissolve polar solute.
Also, non-polar solvent will only dissolve non-polar solute.
Vegetable oil is a non-polar solventIt cannot dissolve a polar solute such as vinegarTherefore, the answer is no, vinegar will dissolve in water.
help now plsss I really need help !!!!
Answer:
4
Explanation:
the one you ARE ON
How was Bohr's atomic model different from those of previous scientists?
Answer:
Bohr placed the electrons in distinct energy levels. Rutherford described the atom as consisting of a tiny positive mass surrounded by a cloud of negative electrons. Bohr thought that electrons orbited the nucleus in quantized orbits. Also, rutherfords was just a hypothesis while Bhor took the time to make his an experiment
us
If the mole fraction of NaCl in an
aqueous solution is 0.0927, what is
the weight/weight % (percent by
mass) of NaCl.
Molar Mass
Naci: 58.44 g/mol
H2O: 18.016 g/mol
Answer:
24.9%
Explanation:
According to this question, mole fraction of NaCl in an aqueous solution is 0.0927. This means that the mole percent of NaCl in the solution is:
0.0927 × 100 = 9.27%
Let's assume that the solution contains water (solvent) + NaCl (solute), hence, the mole fraction of water will be;
100% - 9.27% = 90.73%
THEREFORE, it can be said that, NaCl contains 0.0927moles while H2O contains 9.073moles
N.B: mole = mass/molar mass
Given the Molar Mass
NaCl: 58.44 g/mol
H2O: 18.016 g/mol
For NaCl;
0.0927 = mass/58.44
mass = 0.0927 × 58.44
5.42g
For H2O;
9.073 = mass/18.016
mass = 9.073 × 18.016
= 16.35g
Total mass of solution = 16.35g + 5.42g = 21.77g
Mass percent of NaCl = mass of NaCl/total mass × 100
% mass of NaCl = 5.42g/21.77g × 100
= 0.249 × 100
= 24.9%
Answer:
24.9
Explanation:
7. You are about to perform some intricate electrical studies on single skeletal muscle fibers from a gastronemius muscle. But first, you must prepare 6L of a 170 mM NaCl solution in which to bath the isolated muscle during your studies. How many grams of NaCl must you weigh out on the lab balance to prepare this bath solution
Answer:
58.61 grams
Explanation:
Taking The molecular weight of NaCl = 58.44 grams/mole
Determine how many grams of NaCl to prepare the bath solution
first we will calculate the moles of NaCl that is contained in 6L of 170 mM of NaCI solution
= ( 6 * 170 ) / 1000
= 1020 / 1000 = 1.020 moles
next
determine how many grams of NaCl
= moles of NaCl * molar mass of NaCl
= 1.020 * 58.44
= 58.61 grams
Which of the following choices is not evidence supporting the theory of plate tectonics?
Answer:
B
Explanation:
Gravity pulls rain and snow down to Earth from the atmosphere through a paire
process called precipitation Water is pulled from elevated areas such as
mountains and hills into lakes, oceans, and water reserviors. What is this
describing?*
role of gravity in the water cycle
role of gravity in condensation
O
role of gravity in evaporation
role of gravity in precipitation
Hydrogen peroxide is a versatile chemical, its uses include bleaching wood pulp and fabrics and substituting for chlorine in water purification. One reason for its versatility is that it can be either an oxidizing or reducing agent. For the following reactions, identify whether hydrogen peroxide is an oxidizing or reducing agent.
a) H2O2 (aq) + 2 Fe2+ (aq) + 2 H+ + 2H20 (1) + 2 Fe3+(aq)
b) 5 H2O2 (aq) + 2 MnO4 (aq) + 6 H+ (aq) → 8 H20 (1) + 2 Mn2+ (aq) + 5 O2 (g)
Answer:
Explanation:
a )
H₂O₂ (aq) + 2 Fe²⁺ (aq) + 2 H⁺ = 2H20 (l) + 2 Fe³⁺(aq)
Here oxidation number of Fe is increasing from + 2 to + 3 so it is being oxidized . Hence H₂O₂ is acting as oxidizing agent here .
b )
5 H₂O₂ (aq) + 2 MnO₄⁻¹ (aq) + 6 H⁺ (aq) → 8 H20 (l) + 2 Mn⁺² (aq) + 5O₂ (g)
In this reaction, oxidation number of Mn is reducing from + 7 to + 2 so it is being reduced . Here H₂O₂ is acting as reducing agent .
How many grams of sodium chloride should you theoretically produce if you start with 5.00 grams of calcium chloride and excess sodium carbonate? (answer in numbers only - no units or words)
Answer:
5.27 g of NaCl
Explanation:
The balanced equation for the reaction is given below:
Na₂CO₃ + CaCl₂ —> 2NaCl + CaCO₃
Next, we shall determine the mass of CaCl₂ that reacted and the mass of NaCl produced from the balanced equation. This can be obtained as follow:
Molar mass of CaCl₂ = 40 + (35.5×2)
= 40 + 71
= 111 g/mol
Mass of CaCl₂ from the balanced equation = 1 × 111 = 111 g
Molar mass of NaCl = 23 + 35.5
= 58.5 g/mol
Mass of NaCl from the balanced equation = 2 × 58.5 = 117 g
Summary:
From the balanced equation above,
111 g of CaCl₂ reacted to produce 117 g of NaCl.
Finally, we shall determine the theoretical yield of NaCl. This can be obtained as follow:
From the balanced equation above,
111 g of CaCl₂ reacted to produce 117 g of NaCl.
Therefore, 5 g of CaCl₂ will react to produce = (5 × 117)/111 = 5.27 g of NaCl.
Thus, the theoretical yield of NaCl is 5.27 g.