Choose a strategy to clone genes known only by a mutant phenotype in organisms with highly efficient transformation procedures and relatively small genomes.
a. cloning by complementation
b. direct sequencing of the mutant genome
c. PCR of the gene and transformation of an organism
d. cloning using a specific cloning vector

Answers

Answer 1

Answer:

Option a (cloning by complementation) `would be the correct choice.

Explanation:

In species containing extremely efficient transforming techniques as well as comparatively small genomes, cloning by rearrangement testing is performed to clone gene products mostly by a mutated gene as the rearrangement test was being used to detect mutations.This strategy for cloning through mechanism begins with identifying another yeast mutant that affects something like a plasmid representing a cloned genetic material.

The other options in question aren't relevant to the particular circumstance. Therefore the answer to the above seems to be the right one.


Related Questions

Which conclusion can be drawn from the experimental observation that a single strand of DNA contains 2100 dA residues and 1800 dT residues?

Answers

a. There must have been some loss of material in the extraction because the number of dA and dT residues must be the same.

b. This is a palindromic sequence.

c. There must be 2100 dT and 1800 dA residues on the complementary strand.

d. This must be prokaryotic DNA

e. None of these

Answer:

c. There must be 2100 dT and 1800 dA residues on the complementary strand.

When a bacterium such as E. coli is shifted from a warmer growth temperature to a cooler growth temperature, it compensates by

Answers

Question is incomplete, The complete question is as follows:

When a bacterium such as E. coli is shifted from a warmer growth temperature to a cooler growth temperature, it compensates by:

A) increasing its metabolic rate to generate more heat.

B) putting longer-chain fatty acids into its membranes.

C) putting more unsaturated fatty acids into its membranes.

D) shifting from aerobic to anaerobic metabolism.

E) synthesizing thicker membranes to insulate the cell.

Answer:

C) putting more unsaturated fatty acids into its membranes.

Explanation:

Bacteria has the ability to change the composition of fatty acid present in its cell membrane to compensate the change in temperature.

When bacterium E.Coli will shift from hight or warmer temperature to low or cooler temperature, it will put more unsaturated fatty acids into its membranes which will mintain the fluidity in the membrane.

Hence, the correct option is "C".

Which of the following best explains the potential health problems associated with endocrine disruptors? A) Endocrine disruptors are the only available pesticides,hence they are used widely. B) Endocrine disruptors mimic hormones that regulate critical biological processes. C) Endocrine disruptors do not biodegrade, remaining toxic years after their release. D) There are no major health problems associated with exposure to endocrine disruptors. E) Endocrine disruptors encompass a wide array of toxic pesticides.

Answers

B) Endocrine disruptors mimic hormones that regulate critical biological processes.

Endocrine disruptors, at times moreover called as hormonally unique trained professionals, endocrine disturbing engineered substances, or endocrine upsetting blends are manufactured substances that can interfere with endocrine structures. These aggravations can cause threatening developments, birth flees, and other developmental issues.

The potential health problems associated with endocrine disruptors is that Endocrine disruptors mimic hormones that regulate critical biological processes.

For more information:

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Answer:

It's A.) Endocrine disruptors mimic hormones that regulate critical biological processes.

Explanation:

Quizlet gave me the answer

Which of the circumstances below most accurately describes conditions that are likely to permit a robust anti-tumor adaptive immune response?

a. A tumor with high expression of proteins that have mutations in sequences encoding HLA-binding peptides.
b. A robust acute inflammatory response to PAMPs expressed specifically by tumor cells.
c. Anti-tumor T lymphocytes that have high expression of CTLA-4.
d. A tumor with high expression of PD-L1.

Answers

Did you ever figure out the answer to this question?

The study of PAMP-DAMP complexes is vital to the advancement of knowledge regarding inflammatory disorders in general and cancer in particular. Therefore, option (B) is correct.

What is PAMP-DAMP complexes?

Increasing evidence links inflammation to cancer, and at the root of inflammation are PAMPs and DAMPs (DAMPs). Microorganisms contain PAMPs, which are detected by pattern recognition receptors on monocytes and DCs (PRRs). PRR activation produces pro-inflammatory cytokines.

A robust immune response requires endogenous chemicals that pose 'risk' to self-tissues and are created by injured or stressed cells; these are DAMPs, which also trigger inflammation. PAMPs and DAMPs each have about 100 receptors. PAMPs and DAMPs interact; a PRR can bind to both. In this system, PAMPs and DAMPs affect each other's activation threshold. Thus, PAMP-DAMP relationships describe inflammation in a predictable 'inflammatory code'

PAMP-DAMP complexes are key to understanding inflammatory disorders and cancer.

Learn more about adaptive immune response, here:

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Choose only one correct option. Explanation needed.

Answers

Answer: D

only green plants

Answer:

[tex]\large \boxed{\mathrm{D. \ Photosynthesis}}[/tex]

Explanation:

Photosynthesis is a characteristic present in plants. Photosynthesis is a process where plants transform light energy into chemical energy. Reproduction, respiration, and excretion are all characteristics of living organisms.

Some people use the terms "cell cycle" and "mitosis" as if they were the same thing. Which of these best describes why they are not the same?

a. the cell cycle is just a stage of mitosis
b. mitosis and the cell cycle are different types of cell division that occur in different of cells in a multicellular organism
c. mitosis is a sequence of events within the cell cycle that separates the genetic material
d. mitosis is a type of cell division that occurs in eukaryots and the cell cycle takes place in prokaryots

Answers

Answer: C. Cells in meiosis have unique genetic information

Explanation: Meiosis is the cell division that forms four daughter cells from one parent cell. It includes two successive divisions called as meiosis I and meiosis II. Crossing over during prophase I of meiosis I imparts new gene combinations to the daughter cells of meiosis. Hence, daughter cells formed by the end of meiosis have some new gene combinations, that is, unique genetic information.


Evidence without_______
increases its reliability.
a
bias
b
question
с
repetition
d
replication

Answers

Answer:

I'll say its B ) Question

Explanation:

because when your proving something with evidence then you start asking questions (or still have questions) makes it seem like you don't really know.

I need help with this

Answers

Answer:

I know the answer

Explanation:

Yes

Yes

Yes

Aquatic organisms in coastal areas face a variety of predators on land and in the water and air. Which ocean process exposes the coastal aquatic organisms to land-based predators? a. Changing wind patterns alter the ocean currents, changing tide levels. b. Daily tidal patterns leave areas exposed to the land for part of the day. c. The constant wave action generates nutrients that attract predators. d. The ocean conveyor belt raises and lowers the water levels on the coasts.

Answers

Answer:

b

Explanation:

The correct answer as to the ocean process that exposes the coastal organisms to land-based predators would be the daily tidal patterns that leave the coastal area exposed to the land for a part of the day.

Some parts of the coastal areas become exposed to land during certain periods of the day as a result of tidal waves. When this happens, the aquatic organisms occupying these coastal areas become temporarily exposed to land predators who hunt and kill them for food.

Therefore, the correct option is b.

ASAP What is a photosynthetic pigment? What is a photosynthetic pigment? A. An oxygen based compound that captures light energy. B. A light sensitive compound that changes color. C. A colored compound that captures light energy. D. A manmade compound that reacts to light.

Answers

I think the answer is A.

Answer:

A. An oxygen based compound that captures light energy.

What are two major drivers of surface ocean current and deep ocean current? 1. Surface ocean current 2. Deep ocean current The choices are: A. Differences in water density, resulting from the variability of water temperature and salinity B. Global wind systems

Answers

Answer:

1-B 2-A

Explanation:

this is because the wind blowing over the water causes motion whereas deep water is effected by Differences in water density, resulting from the variability of water temperature and salinity


Identify the organelle where photosynthesis takes place.

B

C

D

E

Answers

Answer:

E

Explanation:

If E is the Chloroplasts, where photosynthesis takes place, then the answer is E.

Answer:

E

Explanation:

PLS HELP ME OUT WITH THIS ANSWER........ WHAT IS DINOFLAGELLATES ?

Answers

Answer:

These are single-celled MESOKARYOTES constituting the phylum DINOFLAGELLATA

Explanation:

Their populations are distributed depending on sea surface temperature,e.t.c.

Hope it helps.

• They are basically unicellular, motile, biflagellate and photosynthetic protists. Predominate colour is golden brown but yellow, green, red and even blue also exists.Some Dinoflagellates like Gymnodinium and Gonyaulax grow in large number in the sea and make the water look red and cause the so called “red tide”.

Drag each tile to the correct box. Place the items in order from lowest to highest degree of internal organization. Please answer 1st at the top and last at the botttom

Answers

Answer:

Lowest to highest degree of internal organization form the given items are:

1) Digestive system: Digestive system is a type of organ system, which is consist of several digestive organs such as mouth, stomach, pancrease and intestine.

2) Stomach: Stomach is a type of organn which is a functional group of tissues.

3) Stomach lining tissues: These are functional group of cells.

4) Epithelial stomach cell: cell is a basic unit of all life.

Hence, the correct order form loowest to highest degree of internal organization is:

Digestive system => Stomach => Stomach lining tissues => Epithelial stomach cell

Why do you think premenopausal women need more iron than
men of the same age?

Answers

Answer:

Women need more iron than men to make up for the amount of iron they lose in their menstrual period.

Explanation:

Answer:

Premenopausal women shed blood as part of menstruation every month, which lowers the level of iron in the body. So, they need more iron than men and are also at a greater risk for this nutritional deficiency.

PLATO

Explanation:

Which is a feature of a tonic receptor? Select one: a. The action potential occurs when there is a change in response to a change in condition. b. For this receptor, the stimulus begins with a burst of action potentials. c. They are normally inactive. d. When the stimulus changes the action potential generation changes. e. Provides information about the rate and change of the stimulus. f. The action potential is generated for a short time period.

Answers

Answer:

For this receptor, the stimulus begins with an explosion of action potentials.

that would be the correct option.

Explanation:

A tonic receptor is one that is activated when the action potentials were maintained over time and during the signaling of the receptor.

Tone receptors require continuous stimulation over a period of time to trigger a response and deliver it to the central nervous system.

It keeps the nervous system constantly active in the environment that surrounds it.

They are slowly adaptable, an example of these receptors are the merkel and ruffini receptors.

The ostrich is a bird. However, it has traits that are different from those of the birds we typically see flying around our neighborhoods. Using credible sources, investigate the environment and adaptations of ostriches to explain how they evolved to survive in their environment.

Answers

Answer:

According to National Geographic, ostriches are a part of a very small group of birds that cannot fly because unlike most birds, their small wings are not strong enough to carry their body for flight and their breastbone isn't balanced enough for flying. Birds that are unable to fly are called ratites.

A number of scientists namely Thomas Huxley, Richard Owen, and others have tried to show that these ratites are actually related to each other and eventually, it was discovered that they all had one thing in common, the way the bones at the roof of the mouth were arranged was similar to that of reptiles rather than other birds.

Richard Owen found and assembled the remains of an extinct ostrich skeleton which was an extinct moa and contrary to already held opinion, one ratite known as tinamous did not really fit with the profile of a ratite because it could fly, even though almost grudgingly and they possessed keeled sternum which suggests that they evolved from flying birds.

DNA tests showed that tinamous evolved within ratites and not necessarily as a separate entity. The tests also showed that moas and tinamous are related.

It was also speculated that the division of the supercontinent Pangaea southern side led to the separation of flightless ratite ancestors, causing each landlocked group to evolve and become the flightless birds we know today such as the ostrich, rheas, etc.

Answer:

An ostrich is an extremely large bird that lives in Africa. It can’t fly, but it has powerful legs that allow it to outrun most predators. Its flexible knees and two-toed feet enable it to run easily. An ostrich has strong leg muscles that it can use to kick large predators. It has claws on its wings to defend itself from predators and thick feathers to keep it warm at night. Ostriches have thick eyelids that protect their eyes from sandstorms.

Explanation:

Taxonomists today tend to group organisms into the same category based on similar what?

Answers

Answer:

characteristics

Explanation:

oranisms are grouped based on shared characteristics

Taxonomists today tend to group organisms into the same category based on their evolutionary history and shared ancestry, as explained in Option A, as this allows taxonomists to construct more accurate and detailed phylogenetic trees.

What is taxonomy?

The field of taxonomy is concerned with the classification of living organisms based on their shared characteristics, the aim of taxonomy is to group organisms in a way that reflects their evolutionary history and to provide a standardized system of naming, while earlier taxonomists relied on morphological characteristics, such as the physical appearance of an organism, but modern taxonomists instead use molecular and genetic data to group organisms into categories.

Hence, taxonomists today tend to group organisms into the same category based on their evolutionary histories and shared ancestry, as explained in Option A.

Learn more about the taxonomy here.

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The question is incomplete, complete question is below

Taxonomists today tend to group organisms into the same category based on similar what?

A)based on their evolutionary histories and shared ancestry

B)based on fossils

During cellular movement which filaments will be the ones that are responsible for attaching and pulling the other filaments along?

Answers

Answer:

Myosin

Explanation:

Myosin are the major components of both muscle and non muscle cells in striated muscles which is responsible for contraction or movement that convert chemical energy (ATP) into mechanical work thereby generating force and movement.

During cellular movement , myosin filament will be the ones that are responsible for attaching and pulling the other filaments along because Myosin binds to actin at a binding site on the globular actin protein,it also have ATP binding sites which hydrolyses ADP to ATP , ATP binding causes myosin to release actin, which make actin and myosin to detach from each other thereby causing attachment and contraction of other filaments


is the basic unit of structure and function of living things.

Answers

Answer:

cells

Explanation:

is the basic unit of life :)

A purebred tall pea plant is cross-pollinated with a tall, can you please help
heterozygous pea plant. Use a Punnett square to determine the probability the offspring inherita
recessive short allele. (I point)
75%
25%
0%
50%

Answers

Answer:

50%

Explanation:

because if you use the box method you see that half receive a short alele

   T     t

T TT  Tt

T TT   Tt

Which of the following is an example of a non-mendelian pattern of inheritance? A) all traits are inherited through patterns found by Mendel. B) when individuals with the genotype BB cross, all the offspring have brown fur. C) when pea plants that are hetero-zygous for a certain trait cross some of the offspring express both distinct versions of the trait. D) when horses that are homo-zygous for red hair are crossed with horses that are homo-zygous for white hair, the offspring have both red and white hair.

Answers

The correct answer is B

why does mosquito net easily form lather with hard water ​

Answers

Answer:

Hard water is defined as the type of water that does not readily give a good lather with soap.

Mosquito net do not form easily lather with hard water because hard water is made up of magnesium and calcium ions which precipitate the soap and unable to form lather.

2. Name the sympathetic ganglia in which the preganglionic and postganglionic neurons synapse.

Answers

Answer:

The paravertebral ganglia is the sympathetic ganglia where the pre-ganglionic and post-ganglionic neurons synapse.

Explanation:

Match each part of Sammi's model to the bodily structure it represents. One bodily structure has already been labeled for you.

Answers

Answer:

corrugated cardboard tube - digestive tract

irregular surfaces on the corrugated tube - villi

pump - heart

Plastic tubing - blood vessels

Explanation:

The corrugated cardboard tube represents the digestive tract.  The give away was the presence of the irregular surfaces within the tubes. This is called the villi. The villi is responsible for the absorption of food after digestion into the body.

Irregular surfaces on the corrugated tube represent the villi. They are tiny hair-like structures that are in the small intestine, that are responsible for the absorption of food into the bloodstream.

The pump represents the heart. The heart is the only organ in the body that provides the pressure force that pushes the blood throughout the body. In this model, the pump will most likely be performing the same function.

The plastic tubings are meant to contain one form of fluid or the other. Judging from that, they can be said to represent the blood vessels. This is because they are connected to the heart and are meant to transport bodily fluids around the body

Answer:

corrugated cardboard tube - digestive tractirregular surfaces on the corrugated tube - villipump - heartPlastic tubing - blood vessels

Explanation:

Properties of Water Lab Report

Instructions: Choose a property of water and design an experiment to test the property. Use the following lab template to ensure all lab report components are included.

Title:

Objective(s):
Identify the purpose of your investigation or the question you are attempting to answer. Be sure to tie in the property of water you are testing.

Safety Notes:
Always have parent(s) or guardian(s) permission and supervision when performing a lab activity at home.
Wear proper protective clothing and eyewear when needed.
Be sure to dispose of all materials properly.
Always wash your hands carefully after touching anything in a lab investigation.
Hypothesis:

Variables:
Independent Variable:
Dependent Variable:
Controlled Variable:

Materials:

Procedure:
The procedure should be clear and detailed so that others can repeat it. The details should be specific in how the procedure changes the independent variable, controls all variables that need to be controlled, and observes or measures the resulting changes to the dependent variable.

Data and Observations:
Present all data and observations in a neat and organized manner. Include tables and graphs where appropriate/possible.



Conclusion:
Be sure to answer the following reflection questions as a summary in the conclusion of your lab report:
Was your hypothesis correct? Why or why not?
What were the results of your experiment?
What changes would you make if you were to repeat the experiment?

Questions:
Using what you have learned in the lesson and the experiment, answer the following question in complete sentences.
Analyze the property of water you investigated and provide some real-world applications of the importance of this property of water.

Answers

Answer:

Hypothesis: I think the penny will hold more droplets plain water because adding soap to the water will disrupt surface tension

Independent variable: Penny and water dropper

Dependent variable: plain water

Independent variable:soapy water

Hope this helped

blood consists of which fluid medium
a.lymph
b.platelets
c.plasma
d.all of these

Answers

Answer:

C. Plasma

Explanation:

Blood consists of a fluid medium called Plasma.

Which of the following is an example of reproductive isolation?

Answers

Answer:

the example of reproductive isolation is temporal isolation, ecological isolation, behavioral isolation, mechanical isolation.

all of the following are examples of organic matter soil except

Answers

This question is incomplete; here is the complete question:

All of the following are examples of organic matter soil except

A. Decaying plants

B. Bacteria

C. Fungi

D. Water

The answer to this question is D. Water

Explanation:

Organic matter derives from living beings, due to this, organic matter is considered as a biological product. In this context, materials such as decaying plants are organic matter because they derive from living organisms and contain biological molecules (most contain carbon). This category does not apply to water, which is composed of hydrogen and oxygen and does not derive from living beings. Thus, the one that is not organic matter is water.

Answer:

D. Water

Hope this Helps!! :))

What alternate form of genes do nucleic acids have that allows them to offer variability?

Answers

Answer:

allales

Explanation:

an allele is the variant from a gene

Answer:

A. allales

Explanation:

Other Questions
Discoveries of artistic works from early men and women include all of these except In its first year of operations, Roma Company reports the following. Earned revenues of $57,000 ($49,000 cash received from customers). Incurred expenses of $31,500 ($24,450 cash paid toward them). Prepaid $9,750 cash for costs that will not be expensed until next year.Compute the company's first year net income under both thecash basis and the accrual basis of accounting. Florida Curtain Works is in the process of preparing its budget for next year. Cost of goods sold has been estimated at 60% of sales. Fabric purchases and payments are to be made during the month preceding the month of sale. Wages are estimated at 20% of sales and are paid during the month of sale. Other operating costs amounting to 25% of sales are to be paid in the month following the month of sales. Sales revenue is forecasted as follows:Month SalesFebruary $440,000March $450,000April $480,000May $500,000June $510,000What is the amount of fabric purchases during the month of March?a. $480,000.b. $336,000.c. $288,000.d. $300,000. If the spring constant is 10 N/m and the spring is stretched 1 m, what is the Force? "A customer contributed $20,000 to a variable annuity contract. The account value has grown over the years and the NAV is now $35,000. The customer is now age 60, and takes a lump-sum distribution of $20,000 to pay for expenses. Which statement is TRUE?" Given: AQRS where m2Q = 20 and m2S = 90R1,000 metersQSWhat is the length of segment RS?342 m364 m500 m940 m The joint density function for a pair of random variables X and Y is given. f(x, y) = Cx(1 + y) if 0 x 4, 0 y 4 0 otherwise f(x,y) = 0A) Find the value of the constant C. I already have 1/24.B) Find P(X < = 1, Y < = 1)C) Find P(X + Y < = 1). Originally, filters for Mr. Coffee electric-drip coffeemakers were sold through appliance and mass merchandise stores. If the company decided to sell the filters in grocery stores where customers purchased their coffee, what type of intermediaries would the company most likely hire which approach does procedural programming follow? bottom up, top down, random, or object oriented It seems almost unbelievable that so many students are sick during midterms and final exams, but actually these are times of _________ stress that _________ the effectiveness of the immune system to fight off illness. Group of answer choices please help !!!!!!!!!! Please help ! First one to give correct answer gets brainliest! Though not specifically cited in the producer's contract, the producer is expected to telephone prospects on the insurer's behalf to arrange sales appointments. This is an example of what kind of producer authority? A share of stock is now selling for $110. It will pay a dividend of $8 per share at the end of the year. Its beta is 1. What do investors expect the stock to sell for at the end of the year? Assume the risk-free rate is 4% and the expected rate of return on the market is 15%. (Round your answer to 2 decimal places.) Expected selling price $ Help a friend out I dont understand it what is the lcm of 725 and 325 The three-dimensional figure shown consists of a cylinder and a right circular cone. The radius of the base is 10 centimeters. The height of the cylinder is 16 centimeters, and the total height of the figure is 28 centimeters. The slant height of the cone is 13 centimeters. Which choice is the best approximation of the surface area of the figure? Use 3.14 to approximate pi. Molar mass is reported on the periodic table in the unitsA. kg/m3B. grams/mole.C. moles/gram.D. atoms/gram. Examples of innovation,creativity and entrepreneurship What caused problems during Grant's presidency?O A. ReconstructionO B. Corrupt staff membersO C. The Civil WarXO D. Grant's own dishonesty