You put 10ul (one loopful - the loop is calibrated for this volume) of a 0.03 ug/ul pGLO solution into the DNA tube. Calculate the ug of DNA you put into the tube.

Answers

Answer 1

Answer:

The µg of DNA put into the tube is 0.3 µg.

Explanation:

Here in this question, we are interested in calculating the µg of DNA put into the DNA tube.

This can be calculated using a mathematical formula.

Mathematically;

Total amount of DNA in µg = concentration in µg/ µl X volume in µl

= 10 µg/µl X 0.03µl = 0.3 µg.


Related Questions

What makes up the backbone of a DNA molecule? nucleic acids RNA molecules sugars and phosphates ribose and deoxyribose

Answers

Base, sugar and phosphate and deoxyribose make up a nucleotide on a DNA molecule

Answer:

sugars and phosphates

Explanation:

gradpoint

Use the following scenario to answer the next following question(s):

You and your friends go to the beach for vacation. You all walk down to the beach to go swimming. When you get there, you see the water is murky and green, and there are algae blooms floating on top.

Reference: Ref 6-3

If it is excess nutrients which are feeding the algae blooms and lowering the oxygen content in the water, that process is called:
A. nutrient cycling.
B. nitrification.
C. eutrophication.
D. hypoxia.

Answers

Answer:

eutrophication

Explanation:

Eutrophication refers to a situation in which the aquatic environment becomes excessively enriched with nutrients. This leads to algal blooms in aquatic habitats such as lakes. These nutrients come from Fertilisers used in farming, which find their way into water bodies through run-off thereby increasing nutrient levels.

Excess nutrients causes phytoplankton to grow and reproduce at an alarming rate resulting in algal blooms. This bloom disrupts the balance in the ecosystem leading to many problems.

The algae may end up using all the oxygen in the water, causing oxygen shortage for aquatic life. Some of the algae may die, their decay may lead to further oxygen depletion. As oxygen is depleted, aquatic organisms may also begin to die.

Why are G proteins known as guanine nucleotide-binding protein?

Answers

Answer:

G proteins, also known as guanine nucleotide-binding proteins, are a family of proteins that act as molecular switches inside cells, and are involved in transmitting signals from a variety of stimuli outside a cell to its interior. ... G proteins belong to the larger group of enzymes called GTPases.

In the image, the arrow Is pointing to a celestial object. Which attribute disqualifies the object from being a planet?
A. It appears to have no moons.
B. It is spherical in shape.
C. Its neighborhood is not clear.
D. It has horizontal bands on its surface.

Answers

The correct answer is C. Its neighborhood is not clear.

Explanation:

One of the key features of a planet is that it clears its neighborhood; this means the planet gravitational force is strong enough that there are no other celestial bodies on its orbit or next to it except by its moons or satellites. This does not occur in the image presented because there are smaller celestial bodies near to the main or bigger celestial body. This likely occurs if the celestial body is not big enough to clear its neighborhood, and therefore it is not a planet.

Which of the following best explains the potential health problems associated with endocrine disruptors? A) Endocrine disruptors are the only available pesticides,hence they are used widely. B) Endocrine disruptors mimic hormones that regulate critical biological processes. C) Endocrine disruptors do not biodegrade, remaining toxic years after their release. D) There are no major health problems associated with exposure to endocrine disruptors. E) Endocrine disruptors encompass a wide array of toxic pesticides.

Answers

B) Endocrine disruptors mimic hormones that regulate critical biological processes.

Endocrine disruptors, at times moreover called as hormonally unique trained professionals, endocrine disturbing engineered substances, or endocrine upsetting blends are manufactured substances that can interfere with endocrine structures. These aggravations can cause threatening developments, birth flees, and other developmental issues.

The potential health problems associated with endocrine disruptors is that Endocrine disruptors mimic hormones that regulate critical biological processes.

For more information:

https://brainly.com/question/22965068?referrer=searchResults

Answer:

It's A.) Endocrine disruptors mimic hormones that regulate critical biological processes.

Explanation:

Quizlet gave me the answer

A decrease in muscular activity or damage to neurons that attach to skeletal muscle can lead to a reduction in the size of muscle called

Answers

Answer:

The correct answer is atrophy

Every interaction with the outside world is because of A) epithelial tissue B) connective tissue C) nervous tissue D) muscular tissue

Answers

Answer:

A cutaneous membrane is a multi-layered membrane composed of epithelial and connective tissues. The apical surface of this membrane exposed to the external environment and is covered with dead, keratinized cells that help protect the body from desiccation and pathogens....

A mutation that hides the effect of another mutation at a site that is distinct from the site of the original mutation, but with in the same gene. This mutation is best described as a

Answers

Answer:

The correct answer is intragenic suppressor mutation.

Explanation:

A suppressor mutation partially or completely converses the effects of a different mutation. A suppressor mutation is of two kinds, that is, an intragenic suppressor and an intergenic suppressor. An intragenic suppressor converses the influence of a mutation at a distinct site in a similar gene.  

On the other hand, an intergenic suppressor converses the influence of a mutation at a distinct locus of the gene. It is the intragenic suppressor mutation, which takes place in a similar gene where the occurrence of the first mutation had taken place. Therefore, the mutation, which hides the influence of another mutation at a location, which is different from the location of the original mutation, but taking place in a similar gene can be illustrated as the intragenic suppressor mutation.  

The diagram represents a food pyramid. The concentration of the pesticide DDT in individual
organisms at level D is higher than the concentration in individuals at level A because DDT is
A. produced by organisms at level C ingested by
those in level D
B. passed through levels A, B, and C to organisms
at level D.
C. excreted by organisms at level A as a toxic
waste.
D. synthesized by organisms at level D.

Answers

Answer:

The answer is "Choice b".

Explanation:

In the given question diagram is missing. so first, we define the diagram after that we explain why the above given choice is correct.  

In the attached file the food pyramid can be a divide into the level, in which the D pesticides use the "dichloro-diphenyl-trichloroethane ", which concentration is higher than its entities from the level of A because DDT is transferred with species at level D by levels A, B, and C, that's why the choice "b" is correct.  

RNA polymerase is an enzyme that copies (1 point)
o DNA into DNA.
O RNA into mRNA
O mRNA into tRNA.
DNA into RNA

Answers

Answer:Copies DNA into RNA

Explanation:

A.While the traits studied in Exercise 1 were hypothetical genetic traits, what type of genetic traits do you think are important to study and predict

Answers

Answer:

Genetic disturbances harmful to the organism are genetic characteristics that must be studied and predicted.

Explanation:

Genetic disorders that harm an organism such as sickle cell anemia, hemophilia, Turner syndrome, among others, are important to be studied and predicted, because it will provide better maintenance of that organism. Allowing people who inherit these characteristics to have more efficient treatments and a better life, since the prediction of these characteristics allows the family to establish better adapapitativo methods, that will make the life of this individual easier and with more quality.

QUICKLY! The chemical reaction equation of photosyntesis is shown here: What information is not given in this equation. It does not show if the reaction is reversible. It does not show the reactants needed for the reaction. It does not show that photosynthesis consists of many separate steps. It does not show the number of molecules required to produce glucose.

Answers

Answer:

C: It does not show that photosynthesis consists of many separate steps.

Explanation:

Photosynthesis is a unique process carried out by the cells of autotrophic organisms. It is the process whereby they synthesize their own food in form of sugars (glucose) in the presence of sunlight. Ideally, the photosynthetic process makes use of carbon dioxide (C02) and water (H20) in the presence of light energy (from sun) to produce glucose sugar (C6H12O6) and oxygen (02). The general photosynthetic equation is as follows:

6CO2 + 6H20 + light → C6H12O6 + 6O2

However, the process is not as simple as portrayed as it involves many separate steps that collectively forms the photosynthetic product (glucose). Photosynthesis occurs in two major stages namely: light stage and light independent stage, which in turn consists of series of reactions that forms the products.

Therefore, the equation attached to this question describes photosynthesis but It does not show that photosynthesis consists of many separate steps.

Answer:

C: It does not show that photosynthesis consists of many separate steps.

Explanation:

When the body cells are hypotonic to the blood plasma, water will move from intracellular fluid to extracellular fluid.
A. True
B. False

Answers

Answer:

True

Explanation:

Which sentence about protist is accurate

Answers

Answer:

there are no sentences

Explanation:

why are g protein important for drug discovery?

Answers

Answer:

G protein - coupled  receptors(GPCRs)n belong to a large family of signaling proteins that  mediate cellular  reponses to most hormones ,metabolites,cytokines and neuotransmitters and therefore serve as fritful targets for DRUGS DISCOVERYS.

Explanation: histamine(HRH1),serotonin dopamine,opiod and adrenergic receptors.

I HOPE IT'S HELPS YOU TO UNDERSTAND

The main function of a cell membrane is to allow substances to enter and leave the cell. Which is a correct description of transport through a cell membrane

Answers

Answer:

The question is incomplete, below is the completed question:

The main function of a cell membrane is to allow substances to enter and leave the cell. Which is a correct description of transport through a cell membrane ?

a. Diffusion, or passive transport, occurs when molecules diffuse from a lower concentration to a higher concentration.

b. Facilitated transport occurs when proteins that extend through the cell membrane provide a channel through which certain substances can diffuse more rapidly than by passive diffusion.

c. The lipid bilayer opens up and allows the ions or molecules to enter the cell, then closes.

d. Active transport occurs when molecules diffuse from a higher concentration to a lower concentration.

Answer:

b. Facilitated transport occurs when proteins that extend through the cell membrane provide a channel through which certain substances can diffuse more rapidly than by passive diffusion.

Explanation:

Transport through cell membranes can be broadly classified as active transport or passive transport.

Active transport requires energy in the form of ATP for the transportation of substances across the cell membrane.

Option b correctly describes facilitated diffusion, which is a passive-mediated diffusion process, where channels in the cell membrane mainly composed of proteins called transmembrane integral proteins, allow for the movement of certain molecules (mostly polar/hydrophilic) molecules that will not otherwise be transported through simple diffusion because of the hydrophobic nature of the cell membrane.

The other options are wrong because:

a. diffusion or simple transport involves the movement of substances from regions of higher to a lower concentration not lower to higher as suggested here.

c. The lipid bi-layer does not allow ions or hydrophilic molecules because it is hydrophobic in nature, and it is not a channel, so it does not open and closes

d. In active transport, transfer of molecules is from regions of lower to higher concentration and not higher to lower concentration as suggested here.

I need help with this

Answers

Answer:

I know the answer

Explanation:

Yes

Yes

Yes

____________ a regulatory protein that may bind DNA to inhibit transcription. ____________ a DNA sequence that binds a regulatory protein. ____________a DNA sequence that binds RNA polymerase and regulates transcription. ____________a compound that interacts with another protein or compound to form an active repressor ____________a compound that induces or activates transcription, such as lactose.a. Repressorb. Operator c. Promoterd. Corepressore. Inducer

Answers

Answer:

A repressor is a regulatory protein that may bind DNA to inhibit transcription. An operator is a DNA sequence that binds a regulatory protein. A promoter is a DNA sequence that binds RNA polymerase and regulates transcription. A corepressor is a compound that interacts with another protein or compound to form an active repressor. An inducer is a compound that induces or activates transcription, such as lactose.

Explanation:

Repressor proteins bind to DNA and RNA to suppress target gene expression. A DNA repressor blocks transcription by inhibiting the attachment of RNA polymerase to the promoter sequence, while an RNA repressor bind to the mRNA to block translation of the protein. An operator is a DNA sequence required for the attachment of transcription factors. The genes that are transcribed by the binding of a transcription factor to an operator sequence are collectively referred to as an operon. Operons are generally found in prokaryotes but they also can be found in some eukaryotes including Caenorhabditis elegans and Drosophila melanogaster. An inducer is a molecule that activates gene transcription either by binding to repressors/corepressors (thereby inactivating their functions) or by binding activators. For example, the allolactose act as an inducer of the lac operon.


is the basic unit of structure and function of living things.

Answers

Answer:

cells

Explanation:

is the basic unit of life :)

identify the components ( parts) of DNA

Answers

Answer:

Phosphorus group, a sugar(like deoxyribose or ribose), and a nitrogenous base

Which location is least likely to experience a volcanic eruption? Α. an island hot spot, such as the island of Hawaii B. Hamilton County on the plains of central Texas с. a convergent boundary, as in the Ring of Fire D a volcanic island arc, such as the Aleutian Arc in Alaska

Answers

Answer:

i think that the answer is B. Hamilton County on the plains of central Texas i took the test

Explanation:

Hamilton County on the plains of central Texas is least likely to experience a volcanic eruption. Therefore, option (B) is correct.

What are volcanoes?

Molten rock and gases stored under the surface erupt through a volcano, generating a hill or mountain.

Active, inactive, or extinct volcanoes. Active volcanoes are likely to erupt again. Dormant volcanoes may erupt again. Extinct volcanoes won't erupt.Magma collects inside active volcanoes. The magma chamber's pressure forces it through rock channels and onto the planet's surface.

Volcanic eruptions can be violent or slow-moving. Volcanoes erupt through vents on the sides or a primary entrance at the top. The volcano's morphology depends on eruption rate and magma chemistry. Land and sea volcanoes exist. As lava cools and hardens, underwater volcanoes build mountains and ranges. When volcanoes rise above the ocean, they create islands.

Learn more about volcano, here:

https://brainly.com/question/18058649

#SPJ5

PLEASE ANSWER QUICK!The Greek roots of the word prokaryote mean “before nucleus.” Describe the way that DNA is organized in prokaryotic cells without the help of a nucleus. How does this approach differ from the way that eukaryotic cells organizes their DNA

Answers

Answer:

Prokaryotic cells' DNA are located in the cytoplasm of the cell rather than in the nucleus, like in eukaryotic cells. DNA aids in protein synthesis and determines functions of the cell in cells, regardless of being within the membrane of a nucleus or not.

-----

I hope this helps a little.

The word prokaryote in Greek means before kernel (nucleus). Unlike the eukaryotic cells, the nuclear material is located in the cytoplasm of the cell in a nucleoid.

What are the characteristics of prokaryotic cells?

The prokaryotic cells are the primitive karyons that are defined by the lack of the true nucleus and organelles. Unlike the eukaryotes, the organelles lack the membrane that covers them but has a tough cell wall.

The prokaryotes include archaea and bacteria which are unicellular and microscopic organisms that are simple and have their genetic material organized into nucleoids in the center of the cell. They have the ability to live in harsh conditions.

Therefore, the eukaryotes and prokaryotes differ in the arrangement of the genetic material.

Learn more about prokaryotes, here:

https://brainly.com/question/18348786

#SPJ2

What percentage of the nation’s demand for electricity could be produced in the future by wind energy?

Answers

Answer:

5-10%

hope this is helpful

ABO blood type is examined in a Taiwanese population, and allele frequencies are determined. In the population,
f (IA) = 0.30,
f(IB) = 0.15, and
f (i) = 0.55.
What are the frequencies of the various genotypes and various phenotypes in this population? Assume Hardy-Weinberg equilibrium.

Answers

Answer and Explanation:

Due to technical problems, you will find the answer and explanation in the attached file.

Design a controlled experiment to test the effect of water temperature on goldfish. be sure to include your hypothesis, independent variable, dependent variable as well as experimental group and control group.

Answers

Answer:

In this experiment, indepedent variable will be temperature and dependent variable will be the respiratory rate of goldfish. Temperature affects the respiratory rate of goldfish, as it's respiratory rate decreases with decrease in temperature of water, the experiment is as follows:

Take two glass containers filled with water A and B and put one goldfish in each container.Measure the temperature of the water using a thermometer.Count mouth movement of both the fishes in certain time.Now put some ice in container B that will decrease the temperature of water and measure the temperature again.Now count the mouth movement of both the fishes for the same time it was counted earlier.

The result will be that respiratory rate of goldfish decreases with the decrease in temperature in container B in comparison to container A goldfish.

An experiment meant to determine the cause of an effect, the effect is the independent variable, while the cause is the dependent variable

A controlled experiment to test the effect of water temperature on goldfish is designed as follows:

The experimental group are: The gold fish in a glass Jar X filled with fresh water and with the lid left open (the treatment of temperature reduction is applied to the experimental group)

The control group are: A second gold fish (selected at random) of the same size, in another glass jar Y filled to the same level with fresh water collected from the same source of the first gold fish

Independent variable: The independent variable is the temperature of the water which will be varied by placing ice cube gradually into the glass jar B

Dependent variable: The number times the gold fish gulp air by rising to the surface, and or the number of time goldfish opens its mouth, which indicates that the goldfish is breathing

The hypothesis: The breath rate of goldfish decreases with decrease in temperature because the goldfish metabolic rate decreases and the water holds more dissolved air and therefore oxygen at a reduced temperature

The Experiment Design:

The experiment is conducted by measuring the initial temperature and breathing rate of both fishes

The temperature of the fresh water in jar X is decreased gradually by adding ice cubes and recording the temperature and breathing rate of the goldfish

A similar experiment from an online source (Maryland School improvement website) the following results where obtained

[tex]\begin{array}{|c|cc|} \underline {Breathing \ rate}&&\underline {Water \ Temperature } \\&&\\ (Dependent \ Variable)&&(Independent \ Variable)\ \\&&\\103&&78.8 ^{\circ}F\\78&&68^{\circ}F\\55&&57.2^{\circ}F\\28&&46.4^{\circ}F\\4&&35.6^{\circ}F\end{array}\right][/tex]

From the experiment, it can be seen that the in the experimental group dependent variable, which is the breathing rate of the goldfish reduces as the temperature which is the dependent variable is reduced

Learn more about experimental variables here:

https://brainly.com/question/18177357

Knowledge of the driver mutations underlying cancer has led to targeted therapeutics, such as the protein kinase inhibitor imatinib (trade name Gleevec) in cases of chronic myeloid leukemia. Cancer cells often become resistant to a given drug, so researchers continue searching for new drugs that target proteins that contribute to the cancerous phenotype. One recent promising approach uses drugs that lead to ubiquitination and proteasomal degradation of the target protein. Which of the following mutated proteins are good candidates for this approach?
A) oncogenes
B) proteins with loss-of-function mutations
C) proteins with gain-of-function mutations
D) tumor suppressor genes

Answers

Answer:

C) proteins with gain-of-function mutations

Explanation:

Gain-of-function mutations: In biology, the term "gain-of-function mutation" is described as one of the different types of mutation in which the altered or changed "gene product" consists of an entirely new pattern or molecular function associated with gene expression. However, the "gene-of-function mutations" are being always considered as "Semidominant or Dominant".

In the question above, the correct answer is option C.

(06.03 LC) Which of the following is an example of how HIV can be transmitted from one person to another? Contact between infected blood and a mucus membrane Contact between infected saliva and an open wound Contact between infected blood and skin Contact between infected saliva and lining of mouth

Answers

Answer:

i believe the answer is contact between a infected blood and a mucus membrane

Explanation:

all of the following are examples of organic matter soil except

Answers

This question is incomplete; here is the complete question:

All of the following are examples of organic matter soil except

A. Decaying plants

B. Bacteria

C. Fungi

D. Water

The answer to this question is D. Water

Explanation:

Organic matter derives from living beings, due to this, organic matter is considered as a biological product. In this context, materials such as decaying plants are organic matter because they derive from living organisms and contain biological molecules (most contain carbon). This category does not apply to water, which is composed of hydrogen and oxygen and does not derive from living beings. Thus, the one that is not organic matter is water.

Answer:

D. Water

Hope this Helps!! :))

What complications might arise from genetic screens targeting an organ that differentiates late in development?

Answers

Answer:

No sign of testicular development in boys and breast development in girls.

Explanation:

Complications like no sign of testicular development in boys and breast development in girls occurs if an organ develop very late. There are many causes of this type of complications such as long term illness, eating improper food and disorder of sexual development. Sometime these complications  also occurs due to genetically. These complications should be treated well with medication and use of nutritious food. These medicines increases the performance of sex hormones in order to initiate puberty.

assuming hardy weinberg equilibrium what is the probability that sarah who is phenotypically normal with no family history and tom

Answers

Answer:

The probability of having a disorder is 50 %.

Explanation:

The offspring has that genetic disorder if the Sarah who is phenotypically normal with no family history and tom has a genetic disorder so there is 50 percent chance that the children of Sarah and tom has that genetic disorder. if the disorder linked with Y chromosome of male so this disorder is present in the sons but if the disorder linked with X chromosome of male so this disorder is present in the daughters.

Other Questions
El siguiente diagrama A, B, C, D, E, F denotan islas, y las lneas de unin son puentes. El hombre empieza en A y camina de isla en isla. El hombre no puede cruzar el mismo puente dos veces. Hallar el nmero de maneras que puede hacer su recorrido antes de almorzar.A-B-C-D E-FA esta conectado a B, B a C y C a D. B est conectado a E, C esta conectado a F y hay una linea que conecta E y C For which of the following compound inequalities is there no solution? A. 3m - 12 > 30 and -6m >= 24 B. -6m >= 12 and m + 5 -18 C. -5m < 20 and 6m > -18D. -4m - 10 = 22>= is greater than or equal to We discussed the different types of intermolecular forces in this lesson, which can affect the boiling point of a substance. 1. Which of these has the highest boiling point?A) ArB) KrC) XeD) Ne2. Which substance has the highest boiling point?A) CH4B) HeC) HFD) Cl2 find the roots of the following equation X + 1 whole square minus x square equal to 2 Gabriel, Harris and Ida are members of Jeweled Watches, LLC. What are their options with respect to the management of their firm? Why does it typically cost more money to win a seat on the Texas Supreme Court than a seat on the Texas court of criminal appeals? The Texas legislature has placed a strict cap on the spending for criminal appeals court election campaigns. There are few interest groups that contribute money to the criminal appeals court candidates campaigns, which keeps down the costs of the election. Judges running for the criminal appeals court do not have to be elected in a statewide election. Fewer people are interested in the court of criminal appeals. What predictable body of water was both a source of prosperity also caused damaging floods forthe Egyptian civilization?the Nile Riverthe Tigris RiverThe Amazon Riverthe Euphrates River Given the graph, find an equation for the parabola. A car travels 120m along a straight road that is inclined at 8 to the horizontal. Calculate the vertical distance through which the car rises. (Sin8=0.1392) how many meters are in 250 centimeters What did Mao Zedong do to be considered a villain? In your answer, list at least three of the villains actions and briefly explain their effects. You might need to find outside sources to answer this question. List the sources that you consulted during your research. Residents of four cities are able to vote in an upcoming regional election. A newspaper recently conducted a survey to gauge support for each of the two candidates. The results of the poll are shown in the two-way frequency table below. A food manufacturer reports the following for two of its divisions for a recent year.($millions) Beverage Division Cheese DivisionInvested assets, beginning $ 2,662 $ 4,455 Invested assets, ending 2,593 4,400 Sales 2,681 3,925 Operating income 349 634 1. Compute return on investment.2. Compute profit margin.3. Compute investment turnover for the year.A food manufacturer reports the following for two of its divisions for a recent year. Which equation does NOT graph a line? A) y = 5 B) y = -3x3 C) y = 2/3 x D) y = 8x That 3 in b is an exponent btw The Whistling Straits Corporation needs to raise $74 million to finance its expansion into new markets. The company will sell new shares of equity via a general cash offering to raise the needed funds. The offer price is $45 per share and the company's underwriters charge a spread of 6 percent. If the SEC filing fee and associated administrative expenses of the offering are $825,000, how many shares need to be sold? (Do not round intermediate calculations and enter your answer in dollars, not millions, rounded to the nearest whole number, e.g., 1,234,567.) Which organisms transform nitrogen to a form that is useful to plants?bacteriaanimalsfungihumans Only ------ percent of the food eaten is turned into its own body.*20%12%10%40% Evaluate the expresin 6c-d when c=2 and d=10 I need help? Please help me with this question State 1 difference between 1 way rotary motion and reversible rotary motion