Which of the following BEST describes the template DNA for the positive control PCR tube in our Wolbachia PCR?

a. The positive control tube receives each kind of template DNA: genomic DNAs from insects known to be and predicted to be positive and negative for Wolbachia infection.
b. The positive control tube receives genomic DNA from an insect known to be positive for Wolbachia infection.
c. The positive control tube receives any genomic DNA from a student's sample that is predicted to be infected with Wolbachia.
d. The positive control tube receives water for template DNA.

Answers

Answer 1

Answer: c. The positive control tube receives any genomic DNA from a student's sample that is predicted to be infected with Wolbachia.

Explanation:

Positive PCR control can be defined as the control sample that is used as a benchmark for comparison with the results of the PCR or polymerase chain reaction. It comprises of artificial genome or primers. For amplification of microbial DNA synthetic oligonucleotides are used as targets of each primer and a hydrolysis probe is also used. As it is already known that primer as well as hydrolysis probe has the specificity for the DNA strand so it will help in identification of the specific region or sequence in the DNA structure. Wolbachia DNA can be detected if the positive control consists of samples of students affected by Wolbachia that can give indication of whether the Wolbachia species is the same as that of the one which has caused infection.


Related Questions

The complementary DNA sequence of AATTGGCC is

TTAACCGG
AATTGGCC
GGCCAATT
ATATGCGC

Answers

The first one TTAACCGG

Answer:

TtAaccgg

nznzn

Explanation:

nnsjsbsjjzhz

What is the answer to this

Answers

Answer:

in which class you are please tell

3 shows active transport

A hypothesis that is non-falsifiable is ___________.

Answers

Answer:

Si no es posible refutarla, dicha teoría queda «corroborada», pudiendo ser aceptada provisionalmente, pero no verificada; es decir, ninguna teoría es absolutamente verdadera, sino a lo sumo «no refutada».

Explanation:

Si no es posible refutarla, dicha teoría queda «corroborada», pudiendo ser aceptada provisionalmente, pero no verificada; es decir, ninguna teoría es absolutamente verdadera, sino a lo sumo «no refutada».

Select the correct answer. Oll-rich countries in the Middle East cover about 4% of Earth's total land area but possess about 48% of the world's known oil reserves. What is the main reason for the high concentration of reserves in this part of the world?​

Answers

Answer: The main reason for the high concentration of reserves in this part of the world which is the middle east that covers bout 4% of earth's total land area but possess about 48% of the world's known oil reserves is due to GEOLOGICAL PROCESSES. Hope this answer helps.

The earth is surrounded by a _______________________ of gases called the ________________. The atmosphere is very _________________________ to life on ________________ and does many ______________________ to help protect life and help _____________________ to survive. The atmosphere absorbs the ______________ from the _________________ and keeps the heat ________________ the atmosphere helping the _______________________ to stay warm, called the ___________________ Effect. ?

Answers

The earth is surrounded by a layer of gases called the atmosphere. The atmosphere is very important to life on Earth and does many things to help protect life and help life to survive.

The atmosphere absorbs the heat from the Sun and keeps the heat inside the atmosphere helping the Earth to stay warm, called the Greenhouse Effect.

As máquinas tem como fonte de calor?
A)Um comburente
B)O gás carbônico
C)Uma Bateria
D)Um combustível.

Answers

Answer:

Hola,  tu respuesta es B. Gas Carbonico

Explanation:

I believe it B. I did the test

In tomatoes, red fruit color is dominant to yellow. Round-shaped fruit is dominant to pearshaped fruit. Tall vine is dominant to dwarf vine.
a. Suppose you cross a true-breeding tall plant bearing red round fruit with a true-breeding dwarf plant bearing yellow pear-shaped fruit. Predict the appearance of the generation.
b. Assuming that the genes controlling the three traits are on three different pairs of chromosomes, what are the possible genotypes in the generation?
c. What are the expected phenotypic ratios?

Answers

Answer:

a) Al the offspring will have genotype - TtRrOo

b) Al the offspring will have genotype - TtRrOo

c) Tall, Red and Round

Explanation:

Given -

Red color of fruit is dominant to yellow color of fruit.

Round shape of fruit is dominant to pear shape of fruit

Also, Tall vine is dominant to dwarf vine

Crossing -  true-breeding tall plant bearing red round fruit (TTRROO) * true-breeding dwarf plant bearing yellow pear-shaped fruit (ttrroo)

a) Al the offspring will have genotype - TtRrOo

b) Al the offspring will have genotype - TtRrOo

c) Tall, Red and Round

which of these diseases cannot be controlled by killing the vector?
A. River blindness
B. Malaria
C. Polio
D. Cholera
E. Bilharzia (shistosoma)

Answers

Answer:

malaria

Explanation:

hopes this helps

which of the following would encourage biodiversity in the myakka river basin

Answers

Answer: aquatic plants

Explanation:

please solve this question its easy peasy. science.​

Answers

Predator of snakes : frogs and rabbits

Answer: first is  sparrow ,second is snake third is rabbit fourth is fox and 7 is hawk

Tell me if i am wrong

) A florist obtained a prize winning flower that he wants to breed to be sold. The trait that makes his flower stand out is dominant for a particular trait. In order to successfully breed this flower, he needs to make sure that the dominant trait is pure dominant and not a hybrid. What should the florist do to determine whether or not the flower is pure or hybrid for this trait?

Answers

Answer:

The correct answer is - Test cross.

Explanation:

A test cross is a cross that is made between an organism with an unknown dominant genotype and an organism that purebred recessive genotype for the particular trait. It determines if the unknown genotype of the individual being tested is purebred allele dominant or heterozygous for the trait.

If all offspring in F1 generation has phenotypically dominant trait then the tested organism is pure breed dominant and if half of the offsprings have the phenotypically dominant trait and half show recessive trait then the organism to be tested is heterozygous or hybrid.

The florist will test cross whether or not the flower is pure or hybrid for this trait.

A test cross would be a hybrid between this and an individual with nothing but an undiscovered dominating genotype as well as a creature having a purebred minority genotype for such specific characteristic.It establishes whether the undiscovered genotype of the subject being examined is purebred allele dominant as well as heterozygous for something like the characteristic.

Thus the aforementioned response is correct.

Learn more about test cross here:

https://brainly.com/question/16805498

Describe the function of Chloropasts

Answers

Answer:

Chloroplasts are found in plant cells, they make up the green pigment ( chlorophyll ) which manufactures food for the plant during photosynthesis process.

should the conowingo dam be removed in Maryland. if yes or no explain

Answers

no because dams are manmade to retain water in case of a state emergency

the volume of a human brain is normally about 1200cm3. the osmolarity of solutes in the cerebrospinal fluid surrounding the brain is normally about 300mM. how does the volume of the brain change when the cerebrospinal fluid osmolarity drops to 280mM

Answers

Answer:

yesterday was a temporary password is goes by

Which of these is a long-term human health issue likely caused by air
pollution?
A. Cancer
B. Damaged hearing
C. Dementia
D. Bioremediation

Answers

Answer:

A. Cancer

Explanation:

Since Lung Cancer is a type of Cancer you can get Cancer.

differentiate between the plants of sugarcane and plantain..​

Answers

Sugarcane or sugar cane refers to several species and hybrids of tall perennial grass in the genus Saccharum, tribe Andropogoneae, that are used for sugar production. The plants are 2-6 m (6-20 ft) tall with stout, jointed, fibrous stalks that are rich in sucrose, which accumulates in the stalk internodes. Sugarcanes belong to the grass family, Poaceae, an economically important flowering plant family that includes maize, wheat, rice, and sorghum, and many forage crops. It is native to the warm, temperate tropical regions of India, Southeast Asia, and New Guinea. The plant is also grown for biofuel production, especially in Brazil, as the canes can be used directly to produce ethyl alcohol (ethanol).

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Drag each label to the correct location.
Classify the organisms based on what they eat.

Answers

Answer:

plants---- producer

trees---- producer

earthworm------decomposer

zebra---- consumer

tiger------- consumer

I don't seem to see the last item there clearly

Answer:

plants---- producer

trees---- producer

earthworm------decomposer

zebra---- consumer

tiger------- consumer

I hope this helps

You changed the ______ of the
microscope and observed different structures of cells

Answers

Answer:

resolution

Explanation:

The resolution of a microscope or lens is the smallest distance by which two points can be separated and still be distinguished as separate objects. The smaller this value, the higher the resolving power of the microscope and the better the clarity and detail of the image.

Answer: Magnification

Explanation:

1. 7. The ability to taste the chemical PTC is determined by a single gene in humans with the ability to taste given by the dominant allele T and inability to taste by the recessive allele t. Suppose two heterozygous tasters (Tt) have a large family. Predict the proportion of their children who will be tasters and nontasters. What is the likelihood that their first child will be a taster

Answers

Answer:

3/4 taster; 1/4 non-taster

The likelihood that their first child will be a taster: 75 %

Explanation:

Complete dominance occurs when a dominant gene variant or 'allele' completely masks the expression of the recessive allele in heterozygous individuals (i.e., individuals carrying one copy of the dominant allele and one copy of the recessive allele). In this case, both parents are heterozygous for a single gene trait (i.e., the ability to taste) which is ruled by complete dominance. In consequence, the expected phenotypic ratio in the progeny (F1) will be:  

- Alleles: T (dominant taster allele); t (recessive non-taster allele)

- Parental cross: Tt x Tt

- Punnett Square from this cross:

         T        t

T       TT     Tt

t        Tt       tt

Expected F1 phenotypic ratio: 3/4 taster (i.e., 1/4 TT genotype + 1/2 genotype Tt = 3/4 or 75%); 1/4 non-taster (tt genotype = 1/4 or 25%). Moroever, the chance that the first child has the taster phenotype is 3/4 (75%).

_____releases carbon dioxide as a waste product. *
Chloroplast
O Cytoplasm
Ribosomes
O Mitochondria

Answers

Answer:

Mitochondria

HOPE THIS HELPS YOU...

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What 4 organic compounds is carbon found in?

Answers

Answer:

Carbon is the primary component of macromolecules, including proteins, lipids, nucleic acids, and carbohydrates.

Explanation:

Environmental factors can influence natural selection because they can increase or decrease?
the number of chromosomes in an individual.
O the number of chromosomes in a population.
the amount of genetic variation in an individual.
O the amount of genetic variation in a population.
оо

Answers

Answer:

...

Explanation:

.......................

Answer:   the amount of genetic variation in a population.

Explanation: :)

Which of these is not a function of the cardiovascular system?
A)controlling many body processes with nerve cells
B)transporting oxygen to cells
C)carrying carbon dioxide to be d)excreted (transferred out)
carrying glucose and other needed materials to cells in your body

Answers

Answer:

B)

Explanation:

CirculaTory system transports oxygen to the cells.

Which statement describes the role of a mitochondrion in providing a body with energy?

It stores glucose that is taken from food so that respiration can happen later.
It combines blood and oxygen so that energy can be released from glucose.
It is the site where oxygen combines with small molecules and releases large amounts of energy.
It is the site where food is broken down into small molecules and amounts of energy

Answers

Answer:

It stores glucose that is taken from food so that respiration can happen later.

please help ill give brainliest :)) A car travels a distance of 385 km in 8 hours. At what speed did it travel?

Answers

Answer:

48.12mph

Explanation:

385km/8hrs=48.12mph

major types of human and plants tissues

Answers

Major types of human tissues:

Epithelium tissueConnective tissueMuscle tissueNervous tissue

Major types of plants tissues:

Permanent tissueMerismatic tissue

Mitochondria and chloroplasts have a number of features in common. Which of the following is NOT one of them?

Answers

Answer:

give options.............

A slow change in a species over many generations is

Answers

Answer:

micro evolution

Explanation:

The answer is micro evolution

A slow change in a species over many generations is called [tex]\sf\purple{evolution}[/tex].

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How is blood different after it is pumped through the capillaries in the intestines?

A. it has less oxygen
B. it has more oxygen

Answers

Answer:

B

Explanation:

It has more oxygen blood different after it is pumped through the capillaries in the intestines. Thus the correct option is A.

What is capillaries?

Capillaries are delicate blood vessels that exist throughout body. They transport blood, nutrients and oxygen to cells in your organs and body systems. Capillaries are the smallest blood vessels in your vascular system.

Nutrients are absorbed then it goes into the bloodstream through capillaries. These nutrients include acids, vitamins and fatty acids.

For more information regarding capillaries, visit:

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1. This part of the electromagnetic spectrum passes though lead and has the highest energy of all emissions.

Answers

The answer is Gamma

Gamma rays have so much penetrating power that several inches of a dense material like lead

1. This part of the electromagnetic spectrum passes though lead and has the highest energy of all emissions.

A. Microwave

B. Radio

C. X-Ray

D. Gamma

Answer: D. Gamma Rays

Gamma rays have the highest energies and shortest wavelengths on the electromagnetic spectrum.

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