The vector allows for protein expression in E. coli.

Briefly explain the role of the following elements in the context of gene expression and protein production:

1- LacP:

2- LacO:

3- LacZa:

4-Briefly explain and indicate what is required for high expression in E. coli in this context..

The vector also contains other important elements. Explain their role:

5- AmpR:

6- ori:

Answers

Answer 1

Answer:

1. Lac P or the promoter is the binding site for RNA polymerase, situated just upstream to the gene.  

2. Lac O or operator is a sequence situated just downstream to the sequence of the promoter at which binding of the repressor protein is done and ceases RNA polymerase enzyme from binding.  

3. Lac Z refers to the gene that encodes for protein beta-galactosidase, which further results in the formation of glucose and galactose from lactose.  

4. For high expression in E.coli IPTG is required. It attaches with the lac repressor and discharges the tetrameric repressor from the lac operator in an allosteric fashion, thus, permitting gene transcription in the lac operon.  

5. AmpR is also known as the ampicillin-resistant gene, which is utilized as a selective marker for selection against ampicillin antibiotic.  

6. Ori or the origin of replication refers to the sequence of the genome, which is accountable for the initiation of replication.  


Related Questions

Which sentence about protist is accurate

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Answer:

there are no sentences

Explanation:

Which of the following correctly describes the order of events occurring during a sympathetic nervous system response?

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Answer:

Stimulation of thoracic or lumbar spinal regions; impulse reaches chain ganglion; acetylcholine release

Explanation:

How does the wheat stem rust fungus harm its host plant?

Answers

Answer:

The stem rust fungus produces several different structures during its life cycle. The most obvious of these on wheat are the reddish-colored urediniospores that form on leaves, stems, and heads during the growing season. These spores are spread by wind and infect wheat or barley plants.

Why must you include the water soluble vitamins in your daily diet? They cannot be stored by the body because they are water soluble and are eliminated daily. They help carry oxygen in the blood They help digest fats. They help build body tissue.

Answers

Answer:

They cannot be stored by the body because they are water soluble and are eliminated daily.

Explanation:

What are the
traditional
circulatory
endpoints of
resuscitation
in shock?​

Answers

Explanation:

traditional circulatory endpoints of resuscitation in shock include hypertension,tachycardia,decreased mentation and urine output

Physiology and anatomy problem-solving skills can be vital when you're the one taking a patient's history or doing the initial assessment. If you remember the functions of different organs and their anatomical relationships, you can come up with hypotheses and ask the relevant questions to test them. You will also know what to be alert for when caring for the patient later. A fair, fat, forty-three-year-old woman had been having episodes of griping abdominal pain after fatty meals. One day she ate French onion soup with lots of cheese and suffered severe enough pain that she called in sick. Her supervisor pointed out that she always gets sick after fatty foods. She went to the clinic and the nurse in triage took her vitals and history. The nurse noticed that the whites of her eyes were yellow and that she had tenderness on the right side of her abdomen. Blood pressure and heart rate were normal.
Based on this initial assessment, what organs do you think might be involved in this woman's illness, and why?
Choose the two most likely organs involved in her illness
a. Liver, because of the yellow coloration in her eyes, which indicates that the liver is not processing bilirubin.
b. Spleen, because of the pain on the right side of her abdomen.
c. Gall bladder, because it stores bile and might be related to the bilirubin buildup in her blood.
d. Stomach, because the pain occurs after eating
e. Heart, because her heart rate has not increased in response to pain.
f. Kidneys, because they should be cleaning the wastes out of her blood-her yellow eyes indicate that waste is building up in her blood.
g. Pancreas, because of the elevated blood glucose.

Answers

Answer:

The correct answer is: C. Gallbladder, because it stores bile and could be related to the accumulation of bilirubin in the blood.

Explanation:

The gallbladder is responsible for releasing bile juices that, together with gastrointestinal juices, degrade and metabolize fat molecules.

In people who have an unhealthy life, and gallbladder problems, these juices do not break down fat molecules and this is how these people suffer breakdowns, severe pain in the area of the middle third of the body, floating stools due to the high fat content .

The possible or most frequent problem of people who are like this 40-year-old woman is usually the accumulation of sialoliths in the bile duct.

The bile that is released by the gallbladder is necessary to drain properly into the intestines, if it cannot evacuate due to obliteration of the duct due to sialolithiasis or other reason, the gallbladder can become infected, generating a lot of pain and even possible sepsis.

Answer:

A and C

A: Liver, because of the yellow coloration in her eyes, which indicates that the liver is not processing bilirubin.

C: Gall bladder, because it stores bile and might be realted to tbe billrubin buildup in her blood.

Explanation:

 The combination of trouble with fats and yellow discoloration should make you think about the hepatobiliary system. Now you have a hypothesis about what's wrong with this patient and can make a direct assessment toward supporting or disproving that hypothesis rather than asking a lot of questions that may not be relevant.

What sections of DNA are used in DNA fingerprinting?
O A. Sections that only contain mRNA
O B. Sections that are unique to each person
O c. Sections that have the fewest mutations
O D. Sections that are made by bacteria

Answers

The correct answer is B

You palpate a soft, slightly tender mass in the right scrotum of an adult male. You attempt to reduce the size of the mass, and there is no change in the mass size. Your next assessment maneuver is to:________
a. use two fingers to attempt to reduce the mass.
b. palpate the left scrotum simultaneously.
c. lift the right testicle and then compare pain level.
d. transilluminate the mass

Answers

Answer:

d. transilluminate the mass

Explanation:

Sometimes, painless hard or tender mass develop in the scrotum of men and can cause testicular tumors.

After detecting a scrotal mass, to reduce the size of the mass, the next assessment required is to transilluminate the mass in which testicles will be appear opaque on transillumination. Transillumination of mass will help to detect the orientation of mass which can be treated further.

Hence, the correct answer is d. transilluminate the mass

Is the talc renewable?

Answers

Answer:

no.its nonrenewable.

talc is nonrenewable.

thanks.

Which is the best way to describes the Biodiversity of an areas

Answers

Answer:

Biodiversity is defined as “the variability among living organisms from all sources including, inter alia, terrestrial, marine and other aquatic ecosystems and the ecological complexes of which they are part; this includes diversity within species, between species and of ecosystems.” The importance of this definition ...

After the centromeres separate during mitosis, the chromatids, now called _______, move toward opposite poles of the spindle.

Answers

Answer:

Daughter chromosomes

Explanation:

Based on the data table below, which number would be the BEST choice for the top number of the scale on the vertical axis of a scatter plot showing this data? (PLZ HELP ME UNDERSTAND AND ANSWER THIS:)

Answers

Answer:

D) 70

Explanation:

Vertical is going up so it would the highest number.

If pattern of inheritance is complete dominance what would you expect when a red and yellow fish were crossed

Answers

In the question, it is not mentioned that which fish is dominant and recessive, so lets consider red fish is dominant and yellow fish is recessive.

Answer:

In complete dominance, the dominant trait completely masks the recessive trait and the offspring shows dominant characters only.

When a red and yellow fish were crossed, the pattern of inheritance is complete dominance which means the offspring would have dominant characters.

As we considered, red fish has dominant trait so the offspring produced will also have red color as yellow color (recessive) will be masked by dominant red color fish.

Pectoralis Minor External Obliques Gluteus maximus Hamstrings Which are axial muscles or appendicular muscles?

Answers

Answer:

they are axial muscles

What is the product made by the electron transport chain?
A. Pyruvate
B. ADP
C. ATP
D. Glucose

Answers

Answer:

Hey mate!

The correct answer is option. C.

ATP

Explanation:

the final product made by the electron transport chain is water and ATP.

hope it helped you:)

Answer:

Hey mate the answer is ATP

Explanation:

why do veins have valves

Answers

Unlike arteries, veins contain valves that ensure blood flows in only one direction. (Arteries don't require valves because pressure from the heart is so strong that blood is only able to flow in one direction.) Valves also help blood travel back to the heart against the force of gravity.

Which two layers are approximately the same age?
core sample 1
core sample 2

Answers

Complete question:

Examine the following diagrams. Columns I and II contain rock layers A, B, C, D, E, and F. Both columns were taken from the same dig site at different locations. Which two layers are of approximately the same age? (Note: The diagrams are attached).

Answer:

C and D  

Explanation:  

Layers C and D apparently contain the same fossilized organisms in the same substrate. Their appearance is very similar. By knowing that certain organisms used to live in a certain period of time, if we find two strata that show the same fossilized organisms, then we can estimate that these two strata are the same age. Fossilized organisms are used to estimate the age of a rocky stratum.

Life history can be illustrated through fossilized organisms trapped or converted into rocks. These fossils are known as index or guide fossils, of animal or vegetal nature, and they can apport information about the rocky stratum in which they are located or incrusted. These fossils are used to determine the age of the rocky sediments or the environmental conditions under which they were deposited.

 

Answer:both of them are

Explanation:

The end result of a chemical reaction is always:
A. the formation of new kinds of elements.
B. the production of water molecules.
c. a substance that was not ope of the reactants.
ak
s
D. a molecule that does not have an electric charge.

Answers

Answer:

The formation of new kinds of elements.

When a chemical reaction is finished, a new type of element is made. because the chemicals couldn't react to eachother without making something new.

many endangered species are predators that belong to the higher trophic levels. Which is the process that primarily affects animals at the highest trophic levels?

Answers

Answer:

Top predators at the highest trophic levels eat lower-level predators and herbivores

Explanation:

A trophic level is defined as one group of organisms in the ecosystem occupying the same level in the food chain, which is composed of different levels (primary, secondary and higher levels). Top predators at the higher levels maintain the trophic balance by eating other species that eat on primary producers/consumers, thereby favoring the homeostasis of the ecosystem.

Which of the following is a testable scientific question

Answers

Where are the questions so i can answer them?

Answer: Just took the test: Its "How does the age of city affect the number of trees in the city? "

Sabita is a mother in Nepal. She lives in poverty in the Western part of the country with her two children. Everyone in her family has some degree of malnutrition or nutrient deficiency. Match each of the following deficiency diseases with its corresponding nutrient.

a. Sabita is blind from a diet devoid of fortified milk, animal products, or dark yellow and orange fruits and vegetables.
b. Sabita has an enlarged thyroid gland due to a dependence on unfortified salt.

Nutrient:
1. Iron
2. Vitamin A
3. Vitamin D
4. Iodine

Answers

Answer:

a. Sabita is blind from a diet devoid of fortified milk, animal products, or dark yellow and orange fruits and vegetables. - Vitamin A

Sabita is suffering from Vitamin A deficiency. A lack of Vitamin A usually shows itself with night blindness but can cause complete blindness if not acted upon quickly.

Vitamin A deficiency is quite common in developing or poorer nations such as the area Sabita is located and usually attacks children of women in their reproductive stage. Sources of vitamin A include; fortified milk, animal products and fruits and vegetables.

b.  Sabita has an enlarged thyroid gland due to a dependence on unfortified salt. - Iodine

Iodine deficiency leads to an abnormal enlargement of the thyroid gland leading to a condition known as Goiter which presents itself as a big bulge in a person's neck. Iodine is usually placed in fortified salt along with iron to reduce iodine deficiency so Sabita's dependence on unfortified salt is robbing her of a great source of Iodine.

The condition in which the body is deprived of minerals, vitamins and other macro and micronutrients are called malnutrition.

The correct matches and their explanation are as follows:

a. The correct answer is:

Option 2. Vitamin A

Sabita is blind from meals deficient in fortified milk, animal products, fruits and vegetables due to Vitamin A deficiency.

Night blindness is a common symptom of a Vitamin A deficiency.

It is most common in developing regions due to the lack of proper meals. The best source of vitamin A is milk, fruits and animal products like eggs.

b. The correct answer is:

Option 4. Iodine

Sabita has a swollen thyroid gland due to habituation on unfortified salt deficient in Iodine.

Iodine deficient diet causes thyroid gland enlargement a disease called Goiter. This type of disease also affects glucose metabolism and weight.

To learn more about malnutrition and diet follow the link:

https://brainly.com/question/10789147

The arboreal hypothesis proposes that defining primate characteristics were adaptations to life in the trees, such as Group of answer choices grasping hands, tails, and intelligence. developed vision, greater sense of smell, and grasping hands and feet. grasping hands and feet, developed vision, and greater intelligence. developed vision, greater sense of smell, and tails.

Answers

Answer:

The arboreal hypothesis proposes that defining primate characteristics were adaptations to life in the trees, such as

grasping hands and feet

developed vision

greater intelligence

List three protozoan infections of the circulatory system that are transmitted by arthropods. Include the disease name, specific organism (genus species), vector that is the intermediate between hosts, and disease progress in humans.

Answers

Answer:

List three protozoan infections of the circulatory system that are transmitted by arthropods:

Malaria

Toxoplasmosis

Chagas disease (American trypanosomiasis)

Explanation:

Malaria is a prozotoan infection, that can be produced by several protozoan, in the genus Plasmodium. This Plasmodium infect red blood cells and the vector that trasmites it is the Anopheles mosquitoe. In this disease, red blood cells can have severe damage in different organs because of the hemolysis.

Toxoplasmosis is a disease caused by the protozoan Toxoplasma gondii. The main vector of them are domestic cats. When cats are infected, T. gondii oocysts is found in their feces and it can spread to humans. This disease is generally asymptomatic, except when the patient has AIDS or in pregnancy, which can be very dangerous and fatal.

Chagas disease, or American trypanosomiasis is caused by protozoan Trypanosoma cruzi and is generally transmitted to people thanks to the feces of triatomine bugs. Some of the acute symptoms include fever, vomiting, headache, and in severe cases people can develop a chronic disease. In the worst cases it can produce flaccid cardiomegaly (enlargement of the heart) leading to heart failure and death.

Which type of nuclear power reactor is used in only 1 percent of all power plants?

Answers

Answer:

Breeder reactors

Explanation:

The type of nuclear power reactor that is used in only 1 percent of all power plants would be Breeder reactors.

What is Ependymal cell?

Answers

Answer:

Explanation: Ependymal cell is a type of neuronal support cell the forms the epithelial lining of the brain ventricles in the brain and the central canal of the spinal cord ependymal cells similar to all other neuroglia, are derived from a layer of embryonic tissue known as neuroectoderm.

Ependymal cells are ciliated-epithelial glial cells. It develops from radial glia alongside the surface of the ventricles of the brain and spinal canal.

What is the function of Ependymal cells?

The main role of Ependymal cells is formation of CSF.

The CSF refers to cerebrospinal fluid. It is done by movement of sodium ion, chlorine ion and bicarbonate ion from blood to ventricles of brain.

Inside ventricles of the brain, collection of ependymal cells and capillaries together i.e., the tela choroidea form choroid plexus, which produces the CSF.

Thus, Ependymal cells are involved in formation of CSF.

For more details regarding Ependymal cells, visit:

https://brainly.com/question/3233193

Studies of the relative efficacy or nonefficacy of a drug based on specific genotypes would be considered part of the field of

Answers

Answer:

Personalized genomic medicine  

Explanation:

Personalized genomic medicine is a field that uses genomics data to develop personalized technologies and therapies for clinical care. All people share a sequence homology level higher than 99%; however a very small fraction of the genome (lesser than 1%) contains genetic polymorphisms among individuals, and this variation is associated with the likelihood of suffering diseases, either by increasing or by reducing disease susceptibility. Personalized genomic medicine first identifies genetic variations and then develops new strategies and personalized drugs to treat genetic disorders.

When you touch an object that is very hot you quickly pull your hand back.
What is the correct pathway of nerve impulses for this behavior?

Answers

Answer:

this is called reflect action

Answer:

reflex action

Explanation:

this is because, it makes it possible to swiftly pull back your hand if you accidentally touch a hot pan

Which of the following not an enzyme
mediated process
1) G1
and G2
phases of meiosis
2) S-phase of mitosis
3) Pachytene of meiosis-I
4) None of the above

Answers

Answer:

4) None of the above

Explanation:

Enzymes are known for mediating biological or chemical processes.

Meiosis and mitosis both are part of a biological process called cell cycle or cell division.

The cell cycle is mediated by enzyme cyclins and cyclin-dependent kinases (CDK). cyclin-CDK in G1 phase helps to prepare the cell for S phase. cyclin-CDK also drives Pachytene of meiosis-I

During S-phase of mitosis, ubiquitin ligase helps in mitotic spindle assembly.

Hence, the correct answer is "4) None of the above"

Compare the types of biological macromolecules and their functions.

Answers

there are four main classes

carbohidrates -built from small molecular units that are connected to each other by a strong covelent bonds.

lipids-lipids are molecules that contain hydrocarbons and make up the building blocks of the structure and functions of living cells

protiens -built from small molecular units that are connected to each other by a strong covelent bonds.

nucleic acids -built from small molecular units that are connected to each other by a strong covelent bonds.

Production of maltose, by ___________________ condensation, dehydration, hydrolysis) of starch, in the presence of amylase, can be detected by colorimetric assay.

Answers

Answer:

Hydrolysis.

Explanation:

Maltose is produced by hydrolysis of starch, in the presence of amylase, can be detected by colorimetric assay. As per the assay of hydrolysis of starch we have concluded from the test of enzyme activity of amylase is that maltose being a reducing sugar which reacts and reduces the DNS (3,5-dinitrosalicylic acid) that is basically a pale yellow colored alkaline which results after some minutes into 3-amino,5-nitrosalicyclic acid which orange red colored alkaline after heating.

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