The Gram stain provides a lot of clinically useful information but it won’t give you all the information you need for identification and treatment. About which of these do you NOT learn anything from the Gram stain?

Answers

Answer 1

Answer: d) The ability of the bacteria to process nutrients.

Explanation:

Incomplete question.

Options,

a) Structure of the cell wall b) Bacterial morphology c) Susceptibility to antimicrobial drugs d) The ability of the bacteria to process nutrients.

Gram staining is a type of differential staining to study bacteria, and is used to see cell morphology and to differentiate between gram-positive bacteria that appear violet and gram-negative bacteria that appear pink or red.

To perform the staining, fix the samples with methanol for one minute or heat and add a dye called crystal violet and wait one minute. Then rinse with water and add lugol for one minute. Then add acetone alcohol for 5-30 seconds. At this point, gram-negative bacteria discolor while gram-positive bacteria do not. After this, a contrast staining is performed by adding safranin or basic fuchsin for one minute. This stain will turn the gram-negative bacteria pinkish-reddish.

Crystal violet is a cationic dye that penetrates all bacteria through the bacterial wall. Lugol is a compound consisting of iodine and potassium iodide and acts as a mordant. The iodine enters the bacteria and forms an insoluble complex with the crystal violet, causing the crystal violet to bind more strongly to the bacterial cell wall. The added alcohol and acetone are used for decolorization, since the iodine/violet crystal complex is soluble in the alcohol and acetone. Thus, gram-positive bacteria do not decolorize, while gram-negative bacteria do.

A contrast stain such as safranin or fuchsin is used to highlight gram-negative cells. After contrast staining, gram-negative cells are pink or red, while gram-positive cells remain purple.

The wall of gram-positive bacteria consists of about forty layers of peptidoglycan which does not allow alcohol to pass through it because it is very thick, while that of gram-negative bacteria consists of a single layer of peptidoglycan. These differences cause gram-positive bacteria to retain the violet color and gram-negative bacteria to discolor.

So, with this type of staining we can certainly see the structure of the cell wall, because those that retain the violet dye even after treatment with a decolorant are gram-positive (many layers of peptodoglycan) and those that easily lose the first dye and take up the second are gram-negative (single layer). It also gives us an idea of the shape and morphology, which when stained will be visible under the microscope.

It also informs us about susceptibility to antibiotics, since some act only on gram-positive bacteria and others only on gram-negative bacteria. For example, gram-positive bacteria are sensitive to beta lactamase/penicillinase and gram-negative bacteria are susceptible to broad-spectrum penicillins such as piperacillin. However, this type of staining will not tell us about the bacteria's ability to process nutrients, because that is not a process related to the structure of the cell wall.


Related Questions

An element has an atomic mass number of 16 and an atomic number of 7. The element has protons and neutrons. Reset Next

Answers

the answer of this question is answer b

what is the feature of the eye and it's parts​

Answers

Answer:

1. Cornea

2. Pupil

3. Iris

4. Conjunctiva Glands

5. Lacrimal Glands

Answer:

The cornea, a clear window at the front of the eye, covers the iris and the pupil. A clear lens, located behind the pupil, acts like a camera lens by focusing light onto the retina at the back of the eye. The retina is a light-sensitive inner lining at the back of the eye.

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the function of the marrow of long bones in the body is to?
A.store iron
B. Reduce Friction
C. make blood plasma
D.make red blood cells​

Answers

Answer:

Make red blood cells

Explanation:

Bone marrow is a spongy substance found in the center of the bones. It manufactures bone marrow stem cells and other substances, which in turn produce blood cells. Each type of blood cell made by the bone marrow has an important job. Red blood cells carry oxygen to tissues in the body.

For which of the following phenomena is intrinsic affinity necessarily more important than functional affinity?
A. Antibody-mediated opsonization of encapsulated bacterial pathogens
B. Assay using antibodies to detect small drugs in solution
C. Binding of C1q to immunoglobulin G (IgG) or IgM
D. Binding of neutralizing IgG antibodies to nonenveloped viruses​

Answers

Explanation:

B. Assay using antibodies to detect small drugs in solution

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Hereditary fructose intolerance is a genetic condition caused by a variant in the ALDOB gene, which is critical for fructose metabolism. Infants with this condition do not initially display symptoms, but as they are transitioned from breast milk or formula to solid foods, in particular fruit-based foods, they begin to display symptoms, which include vomiting, diarrhea, and low blood sugar. Individuals who do not consume fructose do not display these symptoms. Which of the following describes this condition?

a. It is incompletely penetrant, due to environmental factors.
b. It has variable expressivity, due to environmental factors.incorrect
c. It is incompletely penetrant, due to genetic factors.
d. It has variable expressivity, due to genetic factors.

Answers

It has variable expressivity due to the environmental factors.

The variation in expressivity occurs due to the environmental factors of that individual. Expressivity describes the differences observed in the phenotype between two individuals that belongs to same genotype.

In the condition, when the patient is allowed to consume the fructose based food, so it expresses the change in its body due to no tolerance against fructose so we can conclude that the change occurs due to environment not due to genetics.

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Which of the following processes
describes a cell undergoing exocytosis?
A. cell eating
B. diffusion
C. removing water and waste
D. moving molecules along a concentration gradient

Answers

Answer:

C. removing water and waste

Explanation:

Exocytosis occurs when a cell produces substances for export, such as a protein, or when the cell is getting rid of a waste product or a toxin.

Hope this helps

Answer:

[tex]\boxed {\boxed {\sf C. \ Removing \ water \ and \ waste}}[/tex]

Explanation:

We are asked to find a description for a cell undergoing exocytosis. First, let's define the process.

Exocytosis is a type of active transport, so ATP or energy is required to initiate and complete the process.

This process moves substances from the inside of a cell to the outside. Molecules are packaged into vesicles. The vesicle moves to the cell membrane and attaches. The vesicle fuses with the membrane and the molecules inside the vesicle are released.

Essentially, substances are removed from the cell. This is especially useful because it can rid the cell of any waste or excess water. Therefore, the correct answer is choice C.

All of the members of a particular species that live in one area are called a(an):
A) biotope
B) community
C) ecosystem
D) biosphere
E) population

Answers

Answer:

Population

Explanation:

population- all the members of one species in a particular area.

Population

Explanation: A population comprises individuals of the same species. ... In short, a population only refers to one species, but a community refers to all living species within a certain area.

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which of the following is a major determinant of the distribution pattern of a population?
1 social interactions
2 habitat area
3 rate of population growth
4 the ages of the individuals

Answers

Answer:

rate of population growth

If the sequence of bases on the mRNA codon is AUU, what would be the sequence of bases on the opposite strand assembled by the tRNA?

A. UUA
B. TAA
C. ATT
D. UAA

Answers

Answer: I know that some people need this so here you go! these are all 100% correct

If the sequence of bases on the mRNA codon is AUU, what would be the sequence of bases on the opposite strand assembled by the tRNA?

Answer: UAA

Which of the following correctly shows the sequence of organelles a protein would take if it were being exported from the cell?

Answer: Ribosome → Rough ER → Vesicle → Golgi Apparatus → Vesicle → Cell Membrane

The endomembrane system is a collection of membrane-bound organelles in eukaryotic cells that handles the production and processing of proteins that will be used in organelles or exported to the outside of the cell. These functions are __________, respectively.

Answer: protein making, protein packaging, and lipid production

In which part of the cell do ribosomes perform protein synthesis?

Answer: cytoplasm

What would happen to a cell that lacked smooth endoplasmic reticulum?

Answer: It would not be able to make lipids.

The sequence of bases on the opposite strand assembled by the tRNA would be UAA.

TRANSFER RNA (tRNA):

Transfer RNA or commonly known as tRNA is a form of RNA that perform two major functions during the process of translation in the ribosome.

Attaching to and reading mRNA sequence using its anticodons. Carrying of corresponding amino acid to the polypeptide chain.

The tRNA posseses a group of three nucleotide sequence called ANTICODON. These anticodons are complementary to the mRNA codon sequence. tRNA uses its anticodon to bind and read the mRNA sequence.

In the RNA; Adenine binds to Uracil (U) while Cytosine (C) binds to Guanine (G).

Hence, if the sequence of bases on the mRNA codon is AUU, the sequence of bases that would be on the anticodon of the tRNA is UAA.

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water can act as either a(n)__or a​

Answers

Explanation:

Water can act as an acid and a base. As an acid, water donates H+, the hydrogen ion. As a base, water donates OH-, the hydroxide ion

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Another name for plasma

Answers

Answer:

life blood

is this it?

Answer:

Blood

Explanation:

Is the liquid component of the blood. Mammalian blood consists of a liquid (plasma) and a number of cellular and cell fragment components.

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Which of the following choices is an example of an independent system

A: a human who needs food and water to survive

B: a plant that needs sunlight to make energy

C:a factory that recycles plastic bottles into reusable tote bags.

D:None of these choices are correct.

Answers

Answer:

B

Explanation:

Answer:

a palnt that need sunlight to make energy

what did Rudolf virchow discovered?

Answers

Answer:

Explanation:

In 1855 Virchow published a statement based on his observations Omnis cellula e cellula, which means that all cells arise from pre-existing cells. This was not a new theory.

What are some items that could be hot enough to bring fuel to
an autoignition temperature

Answers

Answer:

Still higher hydrocarbons are liquid, such gas oil or kerosene. For gas oil or kerosene at ~500 °C/20 bar autoignition time may come down to 1–10 ms (see Fig.

...

Autoignition time and temperature.

Indentify the structures

Answers

Eyes and ears and mouth and nose

what is erythrocytopenia?​

Answers

Answer:

Medical Definition of erythrocytopenia

: deficiency of red blood cells. — called also erythropenia.

Explanation:

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Explanation:

erythrocytopenia is deficiency of RBCs

For those women who are not progressing at all or the baby is showing early signs of distress, the decision is
often made to induce labor. This uses a synthetic form of oxytocin, called Pitocin, which is given intravenously
(IV) to the woman to get the contractions going. It is often stopped after a few hours. Why might that be?

Answers

Answer:

It is common for contractions to occur every often meaning a sign of labor might be happening soon

Explanation:

Simply meaning your brain is telling your system the baby is ready

How do malignant transformed cells differ from normal cells? They are:

Answers

Answer:

In contrast to normal cells, cancer cells don't stop growing and dividing, this uncontrolled cell growth results in the formation of a tumor. Cancer cells have more genetic changes compared to normal cells, however not all changes cause cancer, they may be a result of it

Fill in the bottom line of DNA with the complementary base pair:

Answers

Answer: ACCTGATCGTAGCT

Explanation:

Keep in mind that in DNA replication, the matching base pairs are A and T (adenine and thymine) and C and G (Cytosine and Guanine). This means that for DNA complementary strands, A's can ONLY bind with T's and C's can only bind with G's , and vice versa. So, your answer for the picture you have given is ACCTGATCGTAGCT

HELP ASAP I WILL GIVE BRAINLIST

An unknown solution has a pH of 7.1. Which of these chemicals is likely to increase the pH the most when added to the solution?

HF

KOH

NH3

HNO3

Answers

Answer:

It is most likely KOH

Explanation:

NH3 is classified as a weak base so I'd suspect that to be incorrect although it does have a higher pH

The chemical that is likely to increase the pH the most when added to the solution is KOH. The correct option is B.

What is pH?

pH is the scale for the measurement of acidity and the basicity of any solution. pH is a scale from 1 to 14. The lowest pH will be highly acidic and the highest pH will be the highest basic.

The lowest the pH, it will donate the highest hydronium ions, and with the highest pH, it will dissociate completely into the solution. KOH dissociates completely into ions.

KOH or potassium hydroxide is a strong base. When the base is added to a solution, it increases its basicity. So it will increase the pH of the solution. All others are acids that will lower the pH of the solution.

Thus, the correct option is B. KOH.

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Describe the purpose of mitosis.
2. Describe what is happening in each stage and what each stage looks like:
Prophase
Prometaphase
Metaphase
Anaphase
Telophase
Cytokinesis

Answers

Answer:

To acquire growth and to replace worn out and defected cells.

Explanation:

The main purpose of mitosis is to acquire growth and to replace worn out and defected cells.

In prophase, the chromatin condenses which is present in the nucleus of the cell.

Prometaphase is the stage where the nuclear membrane breaks apart into numerous "membrane vesicles".

Metaphase is the phase where all the genetic material is condensing into chromosomes.

Anaphase is the stage in which chromosomes are pulled by the spindle fibers to opposite poles of the cell.

In Telophase, the cell is nearly dividing, and it starts to re-establish its normal structures as cytokinesis (division of the cell contents) takes place.

Cytokinesis is the process which divides the cytoplasm of a parental cell into two daughter cells.

The rate of capillary filtration and reabsorption are increased when......... .
A) the negative pressure of interstitial fluid is reduced
B) the pressure of oxygen available to tissues is decreased
C) intravenous pressure is increased

Answers

Answer:

Mechanisms of enhanced transcapillary filtration in response to elevations in arterial or venous pressure. Elevations in arterial (Pa) or venous (Pv) pressure increase capillary pressure, which favors enhanced capillary filtration (Jv).

option"c"

What is the metaphor for been weevil

Answers

Answer:

The scarab or dung-beetle, and the spider to some ancient symbologists

What is a small structure within the cell that serves specialized functions?

Answers

Answer:

Organelles

Explanation:

Organelles are small,specialized structures found inside the cell which perform a specific task and help in the functioning of the cell.

Change of a liquid to a gas is ___________.

Answers

Answer:

Condensation. think about I, if you see fog on a lake or pond, that's condensation, if you see water droplets on the side of your cold beverage, that's condensation.

how does the coconut tree have a fibrous, when the tree is strong,but the plants that have fibrous root r week,how is it possible?​

Answers

.Fibrous roots give good anchorage to the coconut plant and also helps in proper absorption of water and minerals.

What were the three large, flightless birds with similar features found on different continents, Australia, Africa, and South America?

Answers

Answer:

Rhea from south america

Emu from Australia

ostrich in Africa

You work in a museum that has a huge, beautiful, stained-glass window
Each panel tints the light a different color. Recently a new exhibit
showcasing plants from around the world has set up a table in front of
the window. Plant 1 is in front of an Orange stained glass, Plant 2 is in
front of a yellow stained glass, and Plant 3 is in front of a purple stained
glass. Which plant should you tell the museum director to move so it
doesn't become malnourished?

A. Plant 2
B. Plant 3
C. Plant 1
D. All 3 plants

Answers

Plant 3 should be removed to prevent it from being malnourished and is denoted as option B.

What is Photosynthesis?

This is the process in which green plants manufacture their food in the presence of sunlight.

The light is not absorbed but reflected as a result of the chlorophyll(green pigment) which is why the plant will be malnourished.

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Please select the correct chorological order for the following events:
A. Treaty of New Echota, Black Hawk War Ends, Black Hawk War Ends
B. Black Hawk War Ends, Treaty of New Echota, Treaty of Fort Laramie
C. Treaty of Fort Laramie, Black Hawk War Ends, Treaty of New Echota
D. Treaty of New Echota, Treaty of Fort Laramie, Black Hawk War Ends
Reset Selection

Answers

Answer:

C treaty of fort laramie, black hawk war end, treaty of new echota

Help anyone please????!

Answers

1. Wavelength
2. Compression
3. Rarefractiom
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