Medicine High School

Question 7 (1 point)
Which of the following describes a change that would result in DNA fragments moving through a gel faster during gel
electrophoresis?
а
b
Increase the voltage of the power supply, which sets up a larger electrical field in the gel and causes the DNA to move faster
Use a higher concentration agarose gel, which would cut the DNA into smaller fragments, causing it to move faster
Use a different restriction enzyme, which would result in longer DNA fragments causing the DNA to move faster
Increase the concentration of the buffer solution, which sets up a larger electrical field in the gel and causes the DNA to move
faster
С
Od

Answers

Answer 1

Answer: A number of factors can affect the migration of nucleic acids: the dimension of the gel pores (gel concentration), size of DNA being electrophoresed, the voltage used, the ionic strength of the buffer, and the concentration of intercalating dye such as ethidium bromide if used during electrophoresis.

Explanation:

Answer 2

The increase in the voltage of the power supply describes a change that would result in DNA fragments moving through a gel faster during gel electrophoresis (Option b).

Gel electrophoresis is a technique used in molecular biology laboratories to separate fragments of DNA according to their molecular sizes.

During gel electrophoresis, an electrical field is applied in order to produce the migration of electrically (negatively) charged fragments of DNA.

The intensity of this electrical field will determine the velocity of separation of the DNA fragments.

In conclusion, the increase in the voltage of the power supply describes a change that would result in DNA fragments moving through a gel faster during gel electrophoresis (Option b).

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