In isoelectric focusing gel electrophoresis a. there is a pH gradient that parallels the electric field gradient b. particular care must be taken to ensure the same pH along the length of the gel c. the electric current is allowed to fluctuate d. the electric circuits of the apparatus must be very well insulated

Answers

Answer 1

Answer:

a. there is a pH gradient that parallels the electric field gradient.

Explanation:

Gel electrophoresis is a technique used to separate DNA fragments according to their size. DNA samples are loaded in the wells and then electric current is applied to pull the DNA fragments out from the gel. Isoelectric gel electrophoresis is a process in which negatively charged DNA fragments are separated from the gel.


Related Questions

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Answers

Answer:

14°F = 263.15 K

31°F = 272.594 K

71°F = 294.817 K

35°F = 274.817 K

Explanation:

Use the formula 273.15 + ((°F–32)•5)/9

A 45.0 kg student runs at a constant velocity up the incline 6 metres high. If the power output of the student is 1.50 x 103 W, how long does it take the student to run the 9.0 m along the incline?

Answers

Answer:

The time taken is 1.76 s.

Explanation:

mass of student, m = 45 kg

height, h = 6 m

power, P = 1500 W

Power is defined as the rate of doing work.

Work done in moving height is

W = m g h

Let the time is t.

P = m g h / t

1500 = 45 x 9.8 x 6 / t

t = 1.76 s

In----- theory, the actual wishes that are threatening to the dreamers conscious awareness are disguised

Answers

Answer:

Unconscious wish fulfillment

Explanation:

Unconscious wish fulfillment

(In the unconscious wish fulfillment theory, the actual wishes of the dreamer that are threatening to the dreamer's conscious awareness are disguised.)

A car has a mass of 2000 kg. While it is traveling along a perfectly flat road, it goes around an unbanked turn that has a radius of 40.0 m. The coefficient of static friction between the car tires and the road is 0.500. The car travels successfully around the turn at a constant speed of 10.0 m/s. Calculate the magnitude of the car's acceleration as it goes around the turn. _______ m/s^2

Answers

Answer:

2.5 m/s²

Explanation:

The given parameters are;

The mass of the car, m = 2,000 kg

The radius of the car, r = 40.0 m

The coefficient of friction between the car tires and the road, μ = 0.500

The constant speed with which the car moves, v = 10.0 m/s

The normal reaction of the road on the car, N = The weight of the car;

∴ N = m × g

Where;

g = The acceleration due to gravity ≈ 9.81 m/s²

N ≈ 2,000 kg × 9.81 m/s² = 19,620 N

The frictional force, [tex]F_f[/tex] = μ × N

The centripetal force, [tex]F_c[/tex] = m·v²/r

The car moves without slipping when [tex]F_f[/tex] = [tex]F_c[/tex]

Therefore, [tex]F_f[/tex] = 0.500 × 19,620 N = 2,000 kg × [tex]v_{max}[/tex]²/40.0 m

∴ [tex]v_{max}[/tex] = √(0.500 × 19,620 N × 40.0 m/2,000 kg) ≈ 14.007 m/s

Therefore, the velocity with which the car moves, v < [tex]v_{max}[/tex]

The cars centripetal acceleration, [tex]a_c[/tex] = v²/r

∴ [tex]a_c[/tex] = (10.0 m/s)²/40.0 m = 2.5 m/s²

The cars centripetal acceleration as it goes round the turn, [tex]a_c[/tex] = 2.5 m/s².

Invisible damage to the body resulting from repeating a movement often is a

Answers

Answer: repetitive strain injury


Explanation: The repetitive strain injury (RSI), also known as overuse syndrome, is defined as the invisible damage to the body resulting from repeating a movement. These type of damage can affect the muscles, nerves, ligaments and tendons.

Can ya pls answer dis rq!

Answers

Answer:

Below

Explanation:

Recommended protein sources

1. Lean meat such as chicken or fish

Advantages : they are high quality sources of protein, and contain all 8 essential amino acids, which is why they are considered a complete protein

2. Animal products like eggs and greek yogurt

Advantages : these foods also contain all 8 essential amino acids, making them a complete protein

3. Pulses like beans and lentils

Advantages : tends to be lower in calories and fat than animal-based protein sources, as well as being higher in fiber and essential nutrients

Hope this helps!

calculate the potential energy stored in a meta ball of mass of 80 kg kept at a height of 15m from the earth surface . What will be the potential energy when the metal ball is kept on the earth surface Take (g- 9.8 m/s] ​

Answers

Answer:

11760 J

Explanation:

cuz potential engery is PE = MHG

SO 80×15×9.8= 11760 J

Answer:

11769j

Explanation:

here,

mass(m)=80 kg

height(h)=15m

acceleration due to gravity(g)=9.8m/s^2

now,

potential energy = m×g×h

= 80×9.8×15

= 11760j

Write down the role played by four digestive juices in digestion and the
organs which secrete them.

Answers

Answer:

Explanation:Digestive juices secreted by various organs such as salivary glands in the mouth, stomach, liver and pancreas help in the process of digestion of food. Saliva secreted by the salivary glands in the mouth aids in the breakdown of starch present in the food to simple sugar

A ball of mass 1.0 kg is rolling due west at 30 m/s. A second ball of mass 2.0 kg moving due north at 6.0 m/s collides with the first ball and they stick together. What is the magnitude of the momentum of the two-ball system immediately after collision

Answers

Answer:

the magnitude of the momentum of the two-ball system immediately after collision is 32.31 kg.m/s

Explanation:

Given;

mass of the first ball, m₁ = 1.0 kg

mass of the second ball, m₂ = 2.0 kg

initial velocity of the first ball, v₁ = 30 m/s due west

initial velocity of the second ball, v₂ = 6 m/s due north

From the principle of conservation of linear momentum;

the total momentum before collision = total momentum after collision

The westward momentum of the first ball, = m₁v₁ = 1 x 30 = 30 kg.m/s

The northward momentum of the second ball = m₂v₂ = 2 x 6 = 12 kg.m/s

The resultant momentum of the two balls;

R² = 30² + 12²

R² = 1044

R = √1044

R = 32.31 kg.m/s

Therefore, the magnitude of the momentum of the two-ball system immediately after collision is 32.31 kg.m/s

please help urgent have you ever seen the old “Pull the tablecloth out from under the dishes” trick? Use Newton’s 1st Law to explain the following:
a. In this trick, are the dishes “objects in motion,” or “objects at rest?”
b. If the dishes end up staying on the table, was an UNBALANCED FORCE applied to them? How do you know?

Answers

Answer:

I kinda dont remember the 1st law so search it up on

and it should tell u , but if u need any help from the

Suppose that people living near a particular​ high-voltage power line have a higher incidence of cancer than people living farther from the power line. Can you conclude that the​ high-voltage power line is the cause of the elevated cancer​ rate? If​ not, what other explanations might there be for​ it? What other types of research would you like to see before you conclude that​ high-voltage power lines cause​ cancer?

Answers

Complete Question

Complete Question is attached below

Answer:

a)Option B

b)Option B

Explanation:

a)

Without any confirmation it will be utterly wrong to conclude that high power voltage is the reason for the elevated cancer cases

Therefore

Option B

b)

The High Concentration of cells volume in a part of the Body is what causes Cancer

Therefore

Option B

Which of the following best describes reverberation?
A.The wave fronts become mixed and broken up due to contact with a rough or
irregular surface.
B. A change in the sound wave velocity causes the wave to bend in a different
direction.
C. A fraction of the sound waves are absorbed by an object and converted to heat
energy.
D. A single sound undergoes several reflections due to multiple reflecting surfaces.

Answers

Reverberation, in psychoacoustics and acoustics, is a persistence of sound after the sound is produced

Explanation:

I think it is right hope its helps

Answer:

D. A single sound undergoes several reflections due to multiple reflecting surfaces.

Explanation:

Sometimes, the source of a sound is surrounded by multiple reflecting surfaces. The waves traveling from the source strike these different surfaces, causing multiple reflections. For example, a single clap of thunder reflects on several clouds and the earth's surface, causing you to hear a rolling rumble instead of a single sound.

give one example where friction is a hindrance. ​

Answers

Answer:

an example of when friction is a hindrance, is if someone with shorts is sliding down a slide. their skin rubbing on the slide will cause friction and will slow them down. another example is if you're dragging a large wooden box across the floor. the wood and the floor interacting cause friction and will make it more difficult to push the box.

Explanation:

hope this helps :D

A variable is a letter that stands in for a number and unit... True or False?

Answers

True it represent two or more numbers

is noise energy a kind of energy or not

Answers

yes. sound waves are produced by energy.

what is the Ramsar convention?

Answers

The Ramsar Convention on Wetlands of International Importance Especially as Waterfowl Habitat is an international treaty for the conservation and sustainable use of wetlands.

One possible unit of speed is ​

Answers

Answer:

option d

Explanation:

Meter per second

Calculate the upthrust on a object immersed in water if the object displace water of mass 2 kg.(g=9.8m/s²) ​

Answers

Thrust is force

Mass=2kgAcceleration due to gravity=g=9.8m/s^2

Using Newtons Second law

[tex]\boxed{\sf Force=Mass\times Acceleration}[/tex]

[tex]\\ \qquad\quad\sf{:}\dashrightarrow Thrust=2(9.8)[/tex]

[tex]\\ \qquad\quad\sf{:}\dashrightarrow Thrust=19.6N[/tex]

We have that the the upthrust on a object immersed in water is

[tex]U=19.6N[/tex]

From the Question we are told that

mass= 2kg

Gravity g=9.8m/s^2

Generally the equation for the upthrust  is mathematically given as

[tex]U=mg[/tex]

Therefore

[tex]U=2*9.8[/tex]

[tex]U=19.6N[/tex]

For more information on this visit

https://brainly.com/question/1519601

Which law states that absolute zero cannot be reached?

Answers

Assuming you mean temperature

Answer: The third law of thermodynamics

Hideki had normal vision for most of his life, but now that he is in his 60s, he has started to have difficulty focusing on near objects. He went to an optometrist, who explained that his vision problem was the result of the lenses in his eyes losing elasticity due to aging. Which condition does Hideki have

Answers

Answer:

He is suffering from hypermetropia.

Explanation:

There are some defects of vision.

Longsightedness of hypermetropia : It is the defect of vision in which the person is not able to see the nearby objects clearly but can see the far off objects clearly. This is due to the elongation of size of eye ball. It is cured by using convex lens of suitable focal length.

Nearsightedness or Myopia : It is the defect of vision in which a person is not able to see the far off objects clearly but can see the nearby objects clearly. It is due to the contraction in the size of eye ball. It is cured by using concave lens of suitable focal length.

So, Hideki is suffering from hypermetropia. So, he should use the convex lens of suitable focal length.  

A glass rod acquires charge by rubbing silk cloth. The charge on the glass is due to:
(a) Friction
(b) Conduction
(c) Induction
(d) Radiation

Answers

Answer:

(a)Friction

Explanation:

It is due to friction as you are rubbing the glass rod with silk cloth  

as friction occurs when 2 surfaces are rubbed against each other

Therefore it is charging by friction

What the suns mass in scientific notation????

Answers

Explanation:

In scientific notation the Sun's mass is: =1.989 x 10 ^30 kg

Answer:

I think in scientific notation the Sun's mass becomes: M Sun = 1.989 x 10 30 kg. The number above the ten, called the power of ten or exponent, stands for the number of decimal places. If it is positive, as in the mass of the Sun, the decimal places are in front of the decimal point.

I hope this help you!:)

importance of estimation in our lives​

Answers

In real life, estimation is part of our everyday experience. For students, estimating is an important skill. First and foremost, we want students to be able to determine the reasonableness of their answer. Without estimation skills, students aren't able to determine if their answer is within a reasonable range.

solve two questions under inclined plane using Newton 2nd law​

Answers

Explanation:

If the mass of the inclined plane is large enough, could N ever be equal to mgcosθ. Reasons?

So far I've come up with: mgcosθ−N=ma meaning if N were to equal mgcosθ, ma=0. Since the surfaces are frictionless and since N is acting on the inclined plane too(it'll have a horizontal component), this isn't possible. Am I right? Where am I going wrong?

help me please i will mark as brainliest

Answers

Answer:

you would be paying $1845.26

how do u calculate the kinetic energy of a ball of mass 0.25kg being kicked vertically upwards with a speed of 5m/s​

Answers

Answer:

3.125J

Explanation:

K.E.= 1/2(mass)(velocity)^2

K.E.=1/2(0.25)(5)^2=3.125

Can I answer my own question

Answers

Answer:

Well okay do it what's your question ?

Answer:

yes

Explanation:

The sky is blue because: Select one: a. the index of refraction for air is slightly larger for blue than for red b. atomic hydrogen is blue c. air molecules tend to polarize light along a horizontal axis d. oxygen and nitrogen molecules are small compared to the wavelengths of visible light e. oxygen molecules have a lower energy ground state than nitrogen molecules

Answers

Answer:

a. the index of refraction for air is slightly larger for blue than for red

can someone please help me with a and b? what do they mean by amplitude?​

Answers

Explanation:

first answer is that the to and fro movement of a pendulum is known as amplitude

please help me with this I really need help​

Answers

Answer:

Ask with your Science teacher

Explanation:

i do not know the ans

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