If bacteria transformed with a recombinant pUC18 plasmid produce white colonies when grown on plates containing ampicillin and Xgal, which of the following is least likely?

a. They produce β-lactamase that provides resistance to ampicillin.
b. They carry a vector that contains a DNA fragment inserted into the multiple cloning site.
c. They produce functional β-galactosidase that cleaves X-gal.
d. They carry a vector with a lacZ gene that has been disrupted and rendered nonfunctional.
e. They carry a vector with the bla gene used as the selectable marker.

Answers

Answer 1

Answer:

c. They produce functional β-galactosidase that cleaves X-gal.

Explanation:

β-galactosidase is an enzyme capable of catalyzing the enzymatic hydrolysis of β-galactosides into monosaccharides by hydrolyzing the β-glycosidic bond. On the other hand, X-gal is an analog of lactose composed of galactose, which is widely used in laboratories for the detection of β-galactosidase. In the presence of β-galactosidase, X-gal is hydrolyzed by cleaving its β-glycosidic bond, thereby confirming the presence of β-galactosidase.


Related Questions

A.While the traits studied in Exercise 1 were hypothetical genetic traits, what type of genetic traits do you think are important to study and predict

Answers

Answer:

Genetic disturbances harmful to the organism are genetic characteristics that must be studied and predicted.

Explanation:

Genetic disorders that harm an organism such as sickle cell anemia, hemophilia, Turner syndrome, among others, are important to be studied and predicted, because it will provide better maintenance of that organism. Allowing people who inherit these characteristics to have more efficient treatments and a better life, since the prediction of these characteristics allows the family to establish better adapapitativo methods, that will make the life of this individual easier and with more quality.

Why are G proteins known as guanine nucleotide-binding protein?

Answers

Answer:

G proteins, also known as guanine nucleotide-binding proteins, are a family of proteins that act as molecular switches inside cells, and are involved in transmitting signals from a variety of stimuli outside a cell to its interior. ... G proteins belong to the larger group of enzymes called GTPases.

What does cyclical mean

Answers

Cyclical means occurring in cycles technically meaning reoccurring.

Which sentence about protist is accurate

Answers

Answer:

there are no sentences

Explanation:

Which location is least likely to experience a volcanic eruption? Α. an island hot spot, such as the island of Hawaii B. Hamilton County on the plains of central Texas с. a convergent boundary, as in the Ring of Fire D a volcanic island arc, such as the Aleutian Arc in Alaska

Answers

Answer:

i think that the answer is B. Hamilton County on the plains of central Texas i took the test

Explanation:

Hamilton County on the plains of central Texas is least likely to experience a volcanic eruption. Therefore, option (B) is correct.

What are volcanoes?

Molten rock and gases stored under the surface erupt through a volcano, generating a hill or mountain.

Active, inactive, or extinct volcanoes. Active volcanoes are likely to erupt again. Dormant volcanoes may erupt again. Extinct volcanoes won't erupt.Magma collects inside active volcanoes. The magma chamber's pressure forces it through rock channels and onto the planet's surface.

Volcanic eruptions can be violent or slow-moving. Volcanoes erupt through vents on the sides or a primary entrance at the top. The volcano's morphology depends on eruption rate and magma chemistry. Land and sea volcanoes exist. As lava cools and hardens, underwater volcanoes build mountains and ranges. When volcanoes rise above the ocean, they create islands.

Learn more about volcano, here:

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(06.03 LC) Which of the following is an example of how HIV can be transmitted from one person to another? Contact between infected blood and a mucus membrane Contact between infected saliva and an open wound Contact between infected blood and skin Contact between infected saliva and lining of mouth

Answers

Answer:

i believe the answer is contact between a infected blood and a mucus membrane

Explanation:

A paleontologist finds a relatively complete skeleton but isn’t sure if it is an ape fossil or a hominid fossil. Which of the following features would NOT help distinguish between the two choices?
a. position of the opening in the skull for the spinal cord
b. design of the pelvis
c. relative length of the hind limbs
d. position of the eyes

Answers

c. relative length of the hind limbs. Overall fossilized apes and hominids are 90+% similar in the past 3.5 million years since Australopithecus afarensis but this one aspect of upper vs. lower limbs is the greatest difference. Hope this helps!

Which conclusion can be drawn from the experimental observation that a single strand of DNA contains 2100 dA residues and 1800 dT residues?

Answers

a. There must have been some loss of material in the extraction because the number of dA and dT residues must be the same.

b. This is a palindromic sequence.

c. There must be 2100 dT and 1800 dA residues on the complementary strand.

d. This must be prokaryotic DNA

e. None of these

Answer:

c. There must be 2100 dT and 1800 dA residues on the complementary strand.

What makes up the backbone of a DNA molecule? nucleic acids RNA molecules sugars and phosphates ribose and deoxyribose

Answers

Base, sugar and phosphate and deoxyribose make up a nucleotide on a DNA molecule

Answer:

sugars and phosphates

Explanation:

gradpoint

QUICKLY! The chemical reaction equation of photosyntesis is shown here: What information is not given in this equation. It does not show if the reaction is reversible. It does not show the reactants needed for the reaction. It does not show that photosynthesis consists of many separate steps. It does not show the number of molecules required to produce glucose.

Answers

Answer:

C: It does not show that photosynthesis consists of many separate steps.

Explanation:

Photosynthesis is a unique process carried out by the cells of autotrophic organisms. It is the process whereby they synthesize their own food in form of sugars (glucose) in the presence of sunlight. Ideally, the photosynthetic process makes use of carbon dioxide (C02) and water (H20) in the presence of light energy (from sun) to produce glucose sugar (C6H12O6) and oxygen (02). The general photosynthetic equation is as follows:

6CO2 + 6H20 + light → C6H12O6 + 6O2

However, the process is not as simple as portrayed as it involves many separate steps that collectively forms the photosynthetic product (glucose). Photosynthesis occurs in two major stages namely: light stage and light independent stage, which in turn consists of series of reactions that forms the products.

Therefore, the equation attached to this question describes photosynthesis but It does not show that photosynthesis consists of many separate steps.

Answer:

C: It does not show that photosynthesis consists of many separate steps.

Explanation:

An sixty-year-old man has painful fluid-filled vesicles that have begun crusting over in a wide band across the left side of his lumbar area. He has a history of chickenpox. Based on this information, the likely disease is which is caused by:________

a. scabies, Pediculosis humanus
b. fever blisters, HSV-1
c. impetigo, Staphylococcus aureus
d. German measles, Rubella
e. shingles, varicella-zoster virus

Answers

Answer: shingles, varicella-zoster virus

Explanation:

It should be noted that shingles usually occur with old people and it causes a painful rash when the chicken pox virus is reactivated in the body.

Since the man is old and has painful fluid-filled vesicles that have begun crusting over in a wide band across the left side of his lumbar area and also has a history of chickenpox, it's very likely it is shingles.

In the image, the arrow Is pointing to a celestial object. Which attribute disqualifies the object from being a planet?
A. It appears to have no moons.
B. It is spherical in shape.
C. Its neighborhood is not clear.
D. It has horizontal bands on its surface.

Answers

The correct answer is C. Its neighborhood is not clear.

Explanation:

One of the key features of a planet is that it clears its neighborhood; this means the planet gravitational force is strong enough that there are no other celestial bodies on its orbit or next to it except by its moons or satellites. This does not occur in the image presented because there are smaller celestial bodies near to the main or bigger celestial body. This likely occurs if the celestial body is not big enough to clear its neighborhood, and therefore it is not a planet.

A mutation that hides the effect of another mutation at a site that is distinct from the site of the original mutation, but with in the same gene. This mutation is best described as a

Answers

Answer:

The correct answer is intragenic suppressor mutation.

Explanation:

A suppressor mutation partially or completely converses the effects of a different mutation. A suppressor mutation is of two kinds, that is, an intragenic suppressor and an intergenic suppressor. An intragenic suppressor converses the influence of a mutation at a distinct site in a similar gene.  

On the other hand, an intergenic suppressor converses the influence of a mutation at a distinct locus of the gene. It is the intragenic suppressor mutation, which takes place in a similar gene where the occurrence of the first mutation had taken place. Therefore, the mutation, which hides the influence of another mutation at a location, which is different from the location of the original mutation, but taking place in a similar gene can be illustrated as the intragenic suppressor mutation.  

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She records the height of both the father and son in inches and obtains the following data. Are sons taller than their fathers? Use the alphaequals0.10 level of significance. Note: A normal probability plot and boxplot of the data indicate that the differences are approximately normally distributed with no outliers.Height of Father Height of Son72.4 77.570.6 74.173.1 75.669.9 71.769.4 70.569.4 69.968.1 68.268.9 68.270.5 69.369.4 67.769.5 6767.2 63.770.4 65.5Which conditions must be met by the sample for this test? Select all that apply.A. The sample size is no more than 5% of the population size.B. The differences are normally distributed or the sample size is large.C. The sample size must be large.D. The sampling method results in a dependent sample.E. The sampling method results in an independent sample.Write the hypotheses for the test. UpperH 0 :H 1 :Calculate the test statistic. t 0=? (Round to two decimal places as needed.)Calculate the P-value. 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Required:a. At what rate was the property tax increasing with respect to time in 2008? b. By how much did the tax change between the years 2008 and 2012? Sheridan Company, had 837000 shares of common stock issued and outstanding at December 31, 2017. On July 1, 2018, an additional 40600 shares of common stock were issued for cash. Sheridan also had unexercised stock options to purchase 31400 shares of common stock at $15 per share outstanding at the beginning and end of 2018. The average market price of Sheridan's common stock was $20 during 2018. What is the number of shares that should be used in computing diluted earnings per share for the year ended December 31, 2018 Identify the sentence that has the subordinating conjunction underlined and is punctuated correctly.A. Please take a few minutes to help me clean up, even if you feel you are too busy.B. We have to finish our work, before we can play a game.C. Although it is not fun to do chores everyone must do their share.D. 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