If an experimenter wants to use the GFP method but needs to detect the presence or absence of several proteins at the same time, he can take advantage of mutational variants of GFP that emit what light?

Answers

Answer 1

Answer:

different colors

Explanation:

The green fluorescent protein (GFP) is a type of protein widely used in molecular biology laboratories because this protein can be used to detect the expression of proteins and to identify cellular structures. This protein displays green fluorescence when it is excited by blue light and, in the last years, many variants of the GFP protein have been developed. The altered GFP proteins react to distinct wavelengths of light, thereby emitting light to different colors. The mutants forms of the GFP protein are produced by genome engineering techniques that generate modifications capable of altering the folding of the normal GFP protein.


Related Questions

Every interaction with the outside world is because of A) epithelial tissue B) connective tissue C) nervous tissue D) muscular tissue

Answers

Answer:

A cutaneous membrane is a multi-layered membrane composed of epithelial and connective tissues. The apical surface of this membrane exposed to the external environment and is covered with dead, keratinized cells that help protect the body from desiccation and pathogens....

b) How will you describe any three (3) major components of the environment to a named
class puyil?​

Answers

Answer:

Hydrosphere, atmosphere and biosphere are the three major components of the environment.

Explanation:

Hydrosphere, atmosphere and biosphere are the three major components of the environment. hydrosphere refers to water bodies such as ocean, sea, ponds and lakes etc that is present in our environment. atmosphere refers to the gaseous layer which is present above the earth surface. in this layer oxygen, nitrogen and carbondioxide etc are present. biosphere refers to all living organisms such as human, animals, plants and microbes etc which are present on earth surface..

Which type of nuclear power reactor is used in only 1 percent of all power plants?

Answers

Answer:

Breeder reactors

Explanation:

The type of nuclear power reactor that is used in only 1 percent of all power plants would be Breeder reactors.

Why are so many of the same genes found in almost all living organisms

Answers

Answer:

Molecular homology occurs when different species inherit similar molecules, such as nucleic acids or proteins, from an evolutionary common ancestor. In many cases, especially for essential housekeeping genes involved in protein synthesis or DNA replication, genes are homologous across most, if not all, living organisms. Hope this helps!

Explanation:

In the image, the arrow Is pointing to a celestial object. Which attribute disqualifies the object from being a planet?
A. It appears to have no moons.
B. It is spherical in shape.
C. Its neighborhood is not clear.
D. It has horizontal bands on its surface.

Answers

The correct answer is C. Its neighborhood is not clear.

Explanation:

One of the key features of a planet is that it clears its neighborhood; this means the planet gravitational force is strong enough that there are no other celestial bodies on its orbit or next to it except by its moons or satellites. This does not occur in the image presented because there are smaller celestial bodies near to the main or bigger celestial body. This likely occurs if the celestial body is not big enough to clear its neighborhood, and therefore it is not a planet.

Each of two parents has the genotype brown/red​, which consists of the pair of alleles that determine hair color​, and each parent contributes one of those alleles to a child. Assume that if the child has at least one brown ​allele, that color will dominate and the​ child's hair color will be brown. a. List the different possible outcomes. Assume that these outcomes are equally likely. b. What is the probability that a child of these parents will have the red/red ​genotype? c. What is the probability that the child will have brown hair color​? a. List the possible outcomes.

Answers

Answer:

a. In image attached.

b. 25% or a 1 in 4 chance.

c. 75% or a 3 in 4 chance.

Explanation:

B = Dominant (brown gene)

r = Recessive (red gene)

If two parents both have Br genes, they will both have brown hair.

The Punnet square will look like the image attached.

Is the talc renewable?

Answers

Answer:

no.its nonrenewable.

talc is nonrenewable.

thanks.

What are the
traditional
circulatory
endpoints of
resuscitation
in shock?​

Answers

Explanation:

traditional circulatory endpoints of resuscitation in shock include hypertension,tachycardia,decreased mentation and urine output

Characteristics of Living Things
7. The basic unit of organization of living things is a(n)
A. atom B. organism
C. cell
D. organ
8. Storing energy obtained from food is an example of
A. evolution B. homeostasis
C. response
D. growth
досвэ ссэ ерэ с
SCB CE CDS c
a cв сссрэ с
свэ ссэ соз с
свэссэ со е
эсвэссэ со с
CB cc CD с
C3 C3 CD
свэссэ CD е
CB CC CD е
CB cC CD е
свэссэ сор
CBS CS D3
СВсса CD с
CBD CCD CD3 G
33 CC.
Ева сср
B3 CCO DOC
Всс CD с
3 cc DSC
93 cca CDC
Во са сра с
35 C3 CDC
ECO Dac
3 C3 CDC
CCO DOC
CCDC
CCS CDS
CCS coac
9. The passing of genes from one generation to another is
A homeostasis
B. response
C. growth D. heredity
10. The main function of reproduction is to
A. have enough male and female to reproduce
B. overpopulate
D. to be able to replace themselves.
C. grow
11. The milkweed plant feeding the caterpillar is an example of
A. interdependency.
C. preparing to die after a long life.
B. reproduction
D. heredity
12. Adaptation is very important to species because it allows them to
A. grow and become successful
C. Die
B. produce offspring better equipped to survive.
D. produce many offspring.

Answers

Answer:

bbbc

Explanation:

all of these were to long so

In most organisms, the end product of glycolysis is pyruvate. Pyruvate still contains a substantial amount of energy, which can be further extracted. Whether the organisms are operating under aerobic or anaerobic conditions determines the metabolic pathway that pyruvate undergoes to produce more ATP. In this tutorial, you will identify the end products of these metabolic pathways.

Answers

Answer:

Pyruvate helps in the production of ATP.

Explanation:

The molecule of pyruvate converted into acetyl CoA. Then each molecule which is produced during glycolysis loses electron and carbondioxide releases. After the breakdown of pyruvate, the electrons loses by pyruvate are transferred to NAD+ in order to produce NADH, which will be used by the cell to produce energy molecule such as ATP. So we can say that pyruvate plays a vital role in the formation of ATP molecule.

Which of the following properties is the temperature at which a liquid turns to gas? (3 points)
оа
Magnetism
Ob
Thermal conductivity
ос
Melting point
Boiling point
Od

Answers

thermal conductivity

Answer:

Boiling point

Explanation:

I did the test

In which of these stages is mitosis most important? A tiger cub is born. A tiger cub learns to walk. A tiger cub begins to grow. A tiger cub play-fights.

Answers

Answer:

A tiger cub begins to grow

Explanation:

because that's when they change the most

Answer:

A tiger club play fights

Explanation:

One main part of mitosis is the replacement of cells, so when the tiger gets hurt fighting, mitosis kicks in to replace the cells.

A research team discovered that a novel hormone X stimulates an enzyme that hydrolyzes proteins in the small intestine. However, when hormone X was added to a test-tube mixture containing the enzyme and proteins, no breakdown occurred.

Required:
a. Why is this the case?
b. Name ONE signal transduction pathway that hormone X is using to transfuse its signal. (write all the steps and intermediates)

Answers

Answer:

a. It is a hormone that acts upstream in the signaling pathway (i.e., acting as a ligand by binding to cell receptors)

b. Dopamine-mediated pathways  

Explanation:

It is well known that many cellular transduction pathways are activated via binding of signaling molecules (ligands) to receptors capable of triggering signaling cascades into the cell. For example, dopamine is a signaling hormone (neurotransmitter) involved in diverse brain processes (i.e., motor control, emotional response, etc). It is a neurotransmitter that binds to dopamine receptors and then stimulates the enzyme adenylate cyclase in a process that subsequently activates the production of the second messenger cyclic adenosine monophosphate (cAMP). Finally, cAMP acts as an intracellular signal transductor that play roles in many biological processes by transferring into the cell the effects of dopamine.

What growth information leads to your answer? The colonies are able to grow on minimal medium containing no additional supplements. The colonies are able to grow on complete medium.

Answers

Answer:

A growth medium or culture medium is a solid, liquid or semi-solid designed to support the growth of a population of microorganisms or cells via the process of cell proliferation,[1] or small plants like the moss Physcomitrella patens.[2] Different types of media are used for growing different types of cells.[3]

The two major types of growth media are those used for cell culture, which use specific cell types derived from plants or animals, and microbiological culture, which are used for growing microorganisms, such as bacteria or fungi. The most common growth media for microorganisms are nutrient broths and agar plates; specialized media are sometimes required for microorganism and cell culture growth.[1] Some organisms, termed fastidious organisms, require specialized environments due to complex nutritional requirements. Viruses, for example, are obligate intracellular parasites and require a growth medium containing living cells.

The diagram represents a food pyramid. The concentration of the pesticide DDT in individual
organisms at level D is higher than the concentration in individuals at level A because DDT is
A. produced by organisms at level C ingested by
those in level D
B. passed through levels A, B, and C to organisms
at level D.
C. excreted by organisms at level A as a toxic
waste.
D. synthesized by organisms at level D.

Answers

Answer:

The answer is "Choice b".

Explanation:

In the given question diagram is missing. so first, we define the diagram after that we explain why the above given choice is correct.  

In the attached file the food pyramid can be a divide into the level, in which the D pesticides use the "dichloro-diphenyl-trichloroethane ", which concentration is higher than its entities from the level of A because DDT is transferred with species at level D by levels A, B, and C, that's why the choice "b" is correct.  

assuming hardy weinberg equilibrium what is the probability that sarah who is phenotypically normal with no family history and tom

Answers

Answer:

The probability of having a disorder is 50 %.

Explanation:

The offspring has that genetic disorder if the Sarah who is phenotypically normal with no family history and tom has a genetic disorder so there is 50 percent chance that the children of Sarah and tom has that genetic disorder. if the disorder linked with Y chromosome of male so this disorder is present in the sons but if the disorder linked with X chromosome of male so this disorder is present in the daughters.

What is the relationship between DNA mutation and sickle-cell anemia? (1 point)
O Sickle-cell anemia and DNA mutations are correlated without any causal relationship.
O Sickle-cell anemia causes a DNA mutation.
O A DNA mutation causes sickle-cell anemia.
O A DNA mutation is correlated with but does not cause sickle-cell anemia.

Answers

Answer:

A DNA mutation causes sickle-cell anemia.

Explanation:

Sickle cell anemia is caused by a single code letter change in the DNA. This in turn alters one of the amino acids in the hemoglobin protein. Valine sits in the position where glutamic acid should be. The valine makes the hemoglobin molecules stick together, forming long fibers that distort the shape of the red blood cells, and this brings on an attack.

What is the maximum magnification of most classroom compound light microscopes? 500x 1,000x 2,500x 5,000x

Answers

Answer:

2,500x

Explanation:

am doing the review rn

Answer:

1,000x

Explanation:

i got yall

Which of the following is not a function of Mitosis? A. Growth and development B. Produce genetically identical daughter cells C. Produce genetically different daughter cells D. Replace damaged cells

Answers

C. Produce genetically different daughter cells, is not a function of mitosis

1. Research 5 functions of the frontal lobe and explain them in detail with example.
2. Explain what you think might happen if someone damaged frontal lobe. You must mention the functions you have listed

Answers

Answer:

Solving a problem, memory, language, judgement,  social and sexual behavior.

Explanation:

5 functions of the frontal lobe are given below:

1) it control the expression of emotions.

2) it is responsible in solving a problem.

3) it also control the memory and language.

4) it helps in judgement.

5) it is responsible for social and sexual behavior.

If frontal lobe is damaged, the individual may lose its memory, judgement ability. the social and sexual behavior is also disturbed. It may lose the ability to solve a problem and also have language problem.

Which two layers are approximately the same age?
core sample 1
core sample 2

Answers

Complete question:

Examine the following diagrams. Columns I and II contain rock layers A, B, C, D, E, and F. Both columns were taken from the same dig site at different locations. Which two layers are of approximately the same age? (Note: The diagrams are attached).

Answer:

C and D  

Explanation:  

Layers C and D apparently contain the same fossilized organisms in the same substrate. Their appearance is very similar. By knowing that certain organisms used to live in a certain period of time, if we find two strata that show the same fossilized organisms, then we can estimate that these two strata are the same age. Fossilized organisms are used to estimate the age of a rocky stratum.

Life history can be illustrated through fossilized organisms trapped or converted into rocks. These fossils are known as index or guide fossils, of animal or vegetal nature, and they can apport information about the rocky stratum in which they are located or incrusted. These fossils are used to determine the age of the rocky sediments or the environmental conditions under which they were deposited.

 

Answer:both of them are

Explanation:

is the following nuclear equation balanced?

Answers

Please write the nuclear equation

PLEASE ANSWER QUICK!The Greek roots of the word prokaryote mean “before nucleus.” Describe the way that DNA is organized in prokaryotic cells without the help of a nucleus. How does this approach differ from the way that eukaryotic cells organizes their DNA

Answers

Answer:

Prokaryotic cells' DNA are located in the cytoplasm of the cell rather than in the nucleus, like in eukaryotic cells. DNA aids in protein synthesis and determines functions of the cell in cells, regardless of being within the membrane of a nucleus or not.

-----

I hope this helps a little.

The word prokaryote in Greek means before kernel (nucleus). Unlike the eukaryotic cells, the nuclear material is located in the cytoplasm of the cell in a nucleoid.

What are the characteristics of prokaryotic cells?

The prokaryotic cells are the primitive karyons that are defined by the lack of the true nucleus and organelles. Unlike the eukaryotes, the organelles lack the membrane that covers them but has a tough cell wall.

The prokaryotes include archaea and bacteria which are unicellular and microscopic organisms that are simple and have their genetic material organized into nucleoids in the center of the cell. They have the ability to live in harsh conditions.

Therefore, the eukaryotes and prokaryotes differ in the arrangement of the genetic material.

Learn more about prokaryotes, here:

https://brainly.com/question/18348786

#SPJ2

What makes up the backbone of a DNA molecule? nucleic acids RNA molecules sugars and phosphates ribose and deoxyribose

Answers

Base, sugar and phosphate and deoxyribose make up a nucleotide on a DNA molecule

Answer:

sugars and phosphates

Explanation:

gradpoint

What are some pros and cons of using a dispersant versus bacteria to deal with a very large spill and how would each one impact the environment

Answers

Answer:

it is toxic to the health

Explanation:

Dispersants create a toxic environment for fish by releasing harmful oil break-down products into the water. ... Dispersants and dispersed oil have also been shown to have toxic effects on bird eggs that are similar or worse than from untreated oil.

Design a controlled experiment to test the effect of water temperature on goldfish. be sure to include your hypothesis, independent variable, dependent variable as well as experimental group and control group.

Answers

Answer:

In this experiment, indepedent variable will be temperature and dependent variable will be the respiratory rate of goldfish. Temperature affects the respiratory rate of goldfish, as it's respiratory rate decreases with decrease in temperature of water, the experiment is as follows:

Take two glass containers filled with water A and B and put one goldfish in each container.Measure the temperature of the water using a thermometer.Count mouth movement of both the fishes in certain time.Now put some ice in container B that will decrease the temperature of water and measure the temperature again.Now count the mouth movement of both the fishes for the same time it was counted earlier.

The result will be that respiratory rate of goldfish decreases with the decrease in temperature in container B in comparison to container A goldfish.

An experiment meant to determine the cause of an effect, the effect is the independent variable, while the cause is the dependent variable

A controlled experiment to test the effect of water temperature on goldfish is designed as follows:

The experimental group are: The gold fish in a glass Jar X filled with fresh water and with the lid left open (the treatment of temperature reduction is applied to the experimental group)

The control group are: A second gold fish (selected at random) of the same size, in another glass jar Y filled to the same level with fresh water collected from the same source of the first gold fish

Independent variable: The independent variable is the temperature of the water which will be varied by placing ice cube gradually into the glass jar B

Dependent variable: The number times the gold fish gulp air by rising to the surface, and or the number of time goldfish opens its mouth, which indicates that the goldfish is breathing

The hypothesis: The breath rate of goldfish decreases with decrease in temperature because the goldfish metabolic rate decreases and the water holds more dissolved air and therefore oxygen at a reduced temperature

The Experiment Design:

The experiment is conducted by measuring the initial temperature and breathing rate of both fishes

The temperature of the fresh water in jar X is decreased gradually by adding ice cubes and recording the temperature and breathing rate of the goldfish

A similar experiment from an online source (Maryland School improvement website) the following results where obtained

[tex]\begin{array}{|c|cc|} \underline {Breathing \ rate}&&\underline {Water \ Temperature } \\&&\\ (Dependent \ Variable)&&(Independent \ Variable)\ \\&&\\103&&78.8 ^{\circ}F\\78&&68^{\circ}F\\55&&57.2^{\circ}F\\28&&46.4^{\circ}F\\4&&35.6^{\circ}F\end{array}\right][/tex]

From the experiment, it can be seen that the in the experimental group dependent variable, which is the breathing rate of the goldfish reduces as the temperature which is the dependent variable is reduced

Learn more about experimental variables here:

https://brainly.com/question/18177357

What percentage of the nation’s demand for electricity could be produced in the future by wind energy?

Answers

Answer:

5-10%

hope this is helpful

RNA polymerase is an enzyme that copies (1 point)
o DNA into DNA.
O RNA into mRNA
O mRNA into tRNA.
DNA into RNA

Answers

Answer:Copies DNA into RNA

Explanation:

Centrioles have pulled the chromosomes all the way to the spindle poles, the process of cleavage furrowing appears to be about to begin. What kind of a cell is this?

Answers

Answer:

An animal cell in the telophase

Explanation:

Telophase is one of the stages of cell division in animal cell .

In the animal cell during telophase, Centrioles have pulled the chromosomes all the way to the spindle poles, the process of cleavage furrowing appears to be about to begin because the nuclear membrane and nucleoli reform, cytokinesis is almost coming to completion and the chromosomes eventually uncoil to chromatin. Usually cytokinesis occurs during telophase.

If the gene encoding one of the proteins that forms cohesin were mutated such that cohesin could not perform its normal function, what would be the consequence? Check all that apply.

Answers

Answer:

The following apply:

- Chromosomes would not be able to condense.

- Chromosomes would not be able to properly align at the metaphase plate.

- The separation of sister chromatids would not likely occur specifically during anaphase A.

- The nuclear envelope would not be able to reform.

Other Questions
the size of ribosome of _________cell is smaller than ribosome of plant cell. A. Animal cell B. fungal cell C.Bacterial cell D. Protists cell In Isosceles trapezoid ABCD, the longer base, AB, is one and one half times as long as the shorter base, CD. The other two sides, AD and BC, are both 13 cm. long. a. If the perimeter is 76 cm. find the lengths of AB and CD. b. If the height of the trapezoid is 12 cm. find its area. (Hint: Area If a number is divided by 3 or 5, the remainder is 1. If it is divided by 7, there is no remainder. What number between 1 and 100 satisfies the above conditions? I really need help Please help me please If the gene encoding one of the proteins that forms cohesin were mutated such that cohesin could not perform its normal function, what would be the consequence? Check all that apply. plz help me i need help In response to increased levels of aldosterone, the kidneys produce In response to increased levels of aldosterone, the kidneys produce urine with a lower concentration of sodium ions. urine with a higher concentration of sodium ions. urine with less glucose. a larger volume of urine. urine with a lower concentration of potassium ions. . A stock is expected to pay a dividend of $0.75 at the end of the year. The required rate of return is rs = 10.5%, and the expected constant growth rate is g = 6.4%. What is the stock's current price which whole number has a factor that is the greatest prime factor between 1 and 30?. 1,593B. 1,247C. 1,311D. 943 BioGrow Pharma Inc. wanted its research partner, an R&D company, to develop a cancer vaccine. However, the project required huge capital investments, and its research partner was not ready to solely face the risks involved. Thus, to gain its partner's confidence and to prove its involvement, BioGrow Pharma invested $100 million in the project. This investment made by BioGrow Pharma will result in a _____. How does suppression of liberties help a dictator get what he wants ? Combine like terms to simplify the expression: 2/5k - 3/5 +1/10k F135H12GseFind mZH to the nearest degree.67O 18O 45O 23 There are $400$ pages in Sheila's favorite book. The average number of words per page in the book is $300$. If she types at an average rate of $40$ words per minute, how many hours will it take to type the $400$ pages of the book? Simplify 27^(-2/3) x 25^(1/2) x 5^0 9 5 9/5 5/9 1500 L has how many significants figures Byrd Corporation is comparing two different capital structures, an all-equity plan (Plan I) and a levered plan (Plan II). Under Plan I, the company would have 205,000 shares of stock outstanding. Under Plan II, there would be 125,000 shares of stock outstanding and $1.73 million in debt outstanding. The interest rate on the debt is 8 percent and there are no taxes. a. Use MM Proposition I to find the price per share. (Do not round intermediate calculations and round your answer to 2 decimal places, e.g., 32.16.) b. What is the value of the firm under each of the two proposed plans? ((Do not round intermediate calculations and enter your answers in dollars, not millions of dollars, rounded to the nearest whole number, e.g., 1,234,567.) which graph represents (x,y)(x,y)left parenthesis, x, comma, y, right parenthesis-pairs that make the equation y = 0.5x+5y=0.5x+5y, equals, 0, point, 5, x, plus, 5 true? White light is spread out into its spectral components by a diffraction grating. If the grating has 1,970 grooves per centimeter, at what angle (in degrees) does red light of wavelength 640 nm appear in first order? (Assume that the light is incident normally on the gratings.) The Smiths are saving money for a down payment on a house. The Smiths have $25,000 in cash, and they estimate that in 5 years they will have approximately $31,000 if they deposit their cash in a savings account that compounds interest yearly. To calculate the $31,000 amount, the Smiths determined