Explain why it is necessary to include only one chain-terminating/synthesis-terminating nucleotide in each well of the electrophoresis instrument.

Answers

Answer 1

Answer:

Explanation:

Generally for the formation of  phosphosdiester bond  between two nucleotide molecules  3'-OH group is needed between the  sugar molecule  of one nucleotide and the  OH group of the sugar present in the other nucleotide above it. However,in the single chain terminating molecule The 3'-OH is lacking.

Hence,DNA polymerace enzyme(which catalysis  DNA replication)  will not  be able to elongate the DNA molecules, through replications with the addition of di-deoxynucleotidetriphosphates (ddNTP). Thus inhibiting replication and therefore chain terminations,


Related Questions

In the box provided explain why living organisms contain more hydrogen atoms than any other atoms yet 65% of a typical organisms mass is oxygen

Answers

Answer:

The prime reason for this is that an atom of oxygen comprises sixteen times more mass in comparison to a hydrogen atom.

Explanation:

This means that A orginasim will appear to contain more oxygen, but the size diffrence will mean that there will be more hydrogen, just of a smaller size with less mass, thus making up a lesser part of the organism in terms of mass, but the majority on the molecular level.

Answer:

Reason 1: The mass of Oxygen is 16 times that of Hydrogen. Hydrogen takes 16 atoms to equal the mass of 1 Oxygen atom.

Reason 2: A large portion of an organism’s mass is in the form of water. Water contains two hydrogen atoms and one oxygen atom.

Explanation:

I just took the test

An area classified as a temperate deciduous forest begins to experience a lack of rainfall that lasts for many years. Given enough time, if the area continues to experience a lack of rainfall, it will eventually become:__________a. savanna.b. temperate rainforest.c. shrubland, grassland, or woodland.d. taiga.

Answers

Answer:

Savanna

Explanation:

Lack of rainfall over a period of time may convert a temperate deciduous forest into a Savanna.

Grasslands are characterized by a domination of grasses rather than large shrubs or trees. Savanna is a grassland with a few scattered individual trees. Savannas are always found in areas that are quite hot. The annual rainfall in a typical savanna may range from about 76.2-101.6 cm. Some savanna regions may receive as low as 15.24 cm -25.4 cm of rain in a year, followed by a long period of drought when fires can occur.

What makes up the backbone of a DNA molecule? nucleic acids RNA molecules sugars and phosphates ribose and deoxyribose

Answers

Base, sugar and phosphate and deoxyribose make up a nucleotide on a DNA molecule

Answer:

sugars and phosphates

Explanation:

gradpoint

Physiology and anatomy problem-solving skills can be vital when you're the one taking a patient's history or doing the initial assessment. If you remember the functions of different organs and their anatomical relationships, you can come up with hypotheses and ask the relevant questions to test them. You will also know what to be alert for when caring for the patient later. A fair, fat, forty-three-year-old woman had been having episodes of griping abdominal pain after fatty meals. One day she ate French onion soup with lots of cheese and suffered severe enough pain that she called in sick. Her supervisor pointed out that she always gets sick after fatty foods. She went to the clinic and the nurse in triage took her vitals and history. The nurse noticed that the whites of her eyes were yellow and that she had tenderness on the right side of her abdomen. Blood pressure and heart rate were normal.
Based on this initial assessment, what organs do you think might be involved in this woman's illness, and why?
Choose the two most likely organs involved in her illness
a. Liver, because of the yellow coloration in her eyes, which indicates that the liver is not processing bilirubin.
b. Spleen, because of the pain on the right side of her abdomen.
c. Gall bladder, because it stores bile and might be related to the bilirubin buildup in her blood.
d. Stomach, because the pain occurs after eating
e. Heart, because her heart rate has not increased in response to pain.
f. Kidneys, because they should be cleaning the wastes out of her blood-her yellow eyes indicate that waste is building up in her blood.
g. Pancreas, because of the elevated blood glucose.

Answers

Answer:

The correct answer is: C. Gallbladder, because it stores bile and could be related to the accumulation of bilirubin in the blood.

Explanation:

The gallbladder is responsible for releasing bile juices that, together with gastrointestinal juices, degrade and metabolize fat molecules.

In people who have an unhealthy life, and gallbladder problems, these juices do not break down fat molecules and this is how these people suffer breakdowns, severe pain in the area of the middle third of the body, floating stools due to the high fat content .

The possible or most frequent problem of people who are like this 40-year-old woman is usually the accumulation of sialoliths in the bile duct.

The bile that is released by the gallbladder is necessary to drain properly into the intestines, if it cannot evacuate due to obliteration of the duct due to sialolithiasis or other reason, the gallbladder can become infected, generating a lot of pain and even possible sepsis.

Answer:

A and C

A: Liver, because of the yellow coloration in her eyes, which indicates that the liver is not processing bilirubin.

C: Gall bladder, because it stores bile and might be realted to tbe billrubin buildup in her blood.

Explanation:

 The combination of trouble with fats and yellow discoloration should make you think about the hepatobiliary system. Now you have a hypothesis about what's wrong with this patient and can make a direct assessment toward supporting or disproving that hypothesis rather than asking a lot of questions that may not be relevant.

After the centromeres separate during mitosis, the chromatids, now called _______, move toward opposite poles of the spindle.

Answers

Answer:

Daughter chromosomes

Explanation:

write a poem about culture
give expiation for your answer
and describe how to write poem
and don't forget to write poem about culture?
please please answer this question ​

Answers

Explanation:my culture is pakistan and it's tradition and colours are well , most people decorate the buses on 14th august people do face painting and make delicious food in world there are many types of cultures and tradition, which people celebrate once in a year

I HOPE WHOEVER YOU ARE LOVE THE POEM AND PLEASE LIKE YOUR CULTURE IN WHATEVER COUNTRY YOU ARE BORN BECUASE IT'S YOUR COUNTRY WHICH IS BEAUTIFUL LIKE MY COUNTRY.

You want to see if mold grows faster on bread in the shade than on bread in the sun. Your experimental variable is _____. mold bread the sun

Answers

Answer:

bread

Explanation:

the exponential variable doesn't change

Which occurs when the body responds to the environment by maintaining a stable internal environment despite changing
external conditions?
spontaneous generation
homeostasis
growth and development
reproduction​

Answers

Answer:

homeostasis

Explanation:

Homeostasis is the biological process by which living organisms maintain a stable internal environmetal condition despite changes in their external environment. Homeostasis is an important phenomenon for the optimal functioning of any living organism.

A vivid example of homeostatic process is the regulation of temperature in humans or some animals. Self-r regulating mechanisms help keep their temperature at a stable state irrespective of temperature changes in the external environment i.e. hot or cold external conditions. The steady state of internal temperature is very important for some physiological processes in living systems.

Answer:

B

Explanation:

Sabita is a mother in Nepal. She lives in poverty in the Western part of the country with her two children. Everyone in her family has some degree of malnutrition or nutrient deficiency. Match each of the following deficiency diseases with its corresponding nutrient.

a. Sabita is blind from a diet devoid of fortified milk, animal products, or dark yellow and orange fruits and vegetables.
b. Sabita has an enlarged thyroid gland due to a dependence on unfortified salt.

Nutrient:
1. Iron
2. Vitamin A
3. Vitamin D
4. Iodine

Answers

Answer:

a. Sabita is blind from a diet devoid of fortified milk, animal products, or dark yellow and orange fruits and vegetables. - Vitamin A

Sabita is suffering from Vitamin A deficiency. A lack of Vitamin A usually shows itself with night blindness but can cause complete blindness if not acted upon quickly.

Vitamin A deficiency is quite common in developing or poorer nations such as the area Sabita is located and usually attacks children of women in their reproductive stage. Sources of vitamin A include; fortified milk, animal products and fruits and vegetables.

b.  Sabita has an enlarged thyroid gland due to a dependence on unfortified salt. - Iodine

Iodine deficiency leads to an abnormal enlargement of the thyroid gland leading to a condition known as Goiter which presents itself as a big bulge in a person's neck. Iodine is usually placed in fortified salt along with iron to reduce iodine deficiency so Sabita's dependence on unfortified salt is robbing her of a great source of Iodine.

The condition in which the body is deprived of minerals, vitamins and other macro and micronutrients are called malnutrition.

The correct matches and their explanation are as follows:

a. The correct answer is:

Option 2. Vitamin A

Sabita is blind from meals deficient in fortified milk, animal products, fruits and vegetables due to Vitamin A deficiency.

Night blindness is a common symptom of a Vitamin A deficiency.

It is most common in developing regions due to the lack of proper meals. The best source of vitamin A is milk, fruits and animal products like eggs.

b. The correct answer is:

Option 4. Iodine

Sabita has a swollen thyroid gland due to habituation on unfortified salt deficient in Iodine.

Iodine deficient diet causes thyroid gland enlargement a disease called Goiter. This type of disease also affects glucose metabolism and weight.

To learn more about malnutrition and diet follow the link:

https://brainly.com/question/10789147

please help...??????

Answers

Answer:

See below

Explanation:

A. Crust (This is the outermost layer of Earth)

B. Inner Core (Innermost part of Earth)

C. Asthenosphere (The layer below lithosphere)

D. Mesosphere (The middlemost layer)

E. Outer Core (The layer above inner core)

F. Mantle (Consists of further three layers)

G. Lithosphere (The layer below crust)

Answer:

[tex]\large \boxed{\mathrm{view \ attachment }}[/tex]

Explanation:

Crust is the topmost layer.

Inner core is the deepest layer near the core.

Asthenosphere is the layer below the lithosphere.

Mesosphere is the layer that makes up most of the mantle.

Outer core is the second deepest layer above the inner core.

Mantle is the largest layer.

Lithosphere is the layer directly below the crust.

Compare the types of biological macromolecules and their functions.

Answers

there are four main classes

carbohidrates -built from small molecular units that are connected to each other by a strong covelent bonds.

lipids-lipids are molecules that contain hydrocarbons and make up the building blocks of the structure and functions of living cells

protiens -built from small molecular units that are connected to each other by a strong covelent bonds.

nucleic acids -built from small molecular units that are connected to each other by a strong covelent bonds.

Volcanic eruptions are events that take place inside Earth’s . When they occur on land, they spew tremendous amounts of gas, releasing matter into the . The hot, flowing lava also disrupts the by destroying any vegetation and animals in its path.

Answers

Answer:

Volcanic eruptions are events that take place inside Earth’s GEOSPHERE. When they occur on land, they spew tremendous amounts of gas, releasing matter into the ATMOSPHERE. The hot, flowing lava also disrupts the BIOSPHERE by destroying any vegetation and animals in its path.

Explanation: I was taking the test and got it right so I thought I could help anyone who needs it

Suppose you want to make a new connection to species in the concept map. Which of the terms below could fill in the blank in this new connection: "_____ is a mechanism that defines the separation between species." Select all that apply.

Answers

Answer:

Hello. You did not enter answer options, but some terms that can be used to fill in the blank are:

Hybrid infertility, Hybrid inviability, Behavioral isolation, Sympatric isolation, Habitat isolation, Hybrid breakdown, Allopatric isolation.

Explanation:

All of the above terms represent situations that cause separation between species. This separation can occur physically or molecularly, but it always results in genetic modifications that end up separating one species genetically from the other, causing evolution and generating different species, to the point that it is not possible to establish mating between its members.

What sections of DNA are used in DNA fingerprinting?
O A. Sections that only contain mRNA
O B. Sections that are unique to each person
O c. Sections that have the fewest mutations
O D. Sections that are made by bacteria

Answers

The correct answer is B

Which is the best way to describes the Biodiversity of an areas

Answers

Answer:

Biodiversity is defined as “the variability among living organisms from all sources including, inter alia, terrestrial, marine and other aquatic ecosystems and the ecological complexes of which they are part; this includes diversity within species, between species and of ecosystems.” The importance of this definition ...

Which process in the carbon cycle is
performed by BOTH plants and animals?
Cellular Respiration
Decomposition
Photosynthesis
Burning (combustion)

Answers

Question :

Which process in the carbon cycle is

Which process in the carbon cycle isperformed by BOTH plants and animals?

Answer:

Cellular Respiration

Decomposition

Photosynthesis

Burning (combustion)

Which of the following not an enzyme
mediated process
1) G1
and G2
phases of meiosis
2) S-phase of mitosis
3) Pachytene of meiosis-I
4) None of the above

Answers

Answer:

4) None of the above

Explanation:

Enzymes are known for mediating biological or chemical processes.

Meiosis and mitosis both are part of a biological process called cell cycle or cell division.

The cell cycle is mediated by enzyme cyclins and cyclin-dependent kinases (CDK). cyclin-CDK in G1 phase helps to prepare the cell for S phase. cyclin-CDK also drives Pachytene of meiosis-I

During S-phase of mitosis, ubiquitin ligase helps in mitotic spindle assembly.

Hence, the correct answer is "4) None of the above"

Assuming that there is no other variation at the sites not represented, how many SNPs are present at this portion of the sequence

Answers

Answer:

1.4 million SNPs are present.

What happen to the cell if nucleus is removed? ​

Answers

Answer:

If the nucleus is removed from the cell, the cell will die as the nucleus is the living component of the cell.

Hope you understand❣

Answer:

Nucleus is the boss of the cell who control all the activities take place in the cell . so if the nucleus is not there no process will be taken and the cell will die .

Hope i helped u mate.

Which of the following can a chemical bond do?
A. Change element properties
B. Store energy
OC. All of these
D. Release energy

Answers

Answer: C

Explanation:

The following can a chemical bond do is all of these. The correct option is C. All of these.

What is a chemical bond?

A chemical bond is a bond formed between two elements. The energy of the reaction is stored in the bonds when the reaction happens, the energy is released from the bonds. It also changes the chemical properties of the elements.

Thus, the correct option is C. All of these.

Learn more about chemical bond

https://brainly.com/question/26176140

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Which of the following correctly describes the order of events occurring during a sympathetic nervous system response?

Answers

Answer:

Stimulation of thoracic or lumbar spinal regions; impulse reaches chain ganglion; acetylcholine release

Explanation:

List three protozoan infections of the circulatory system that are transmitted by arthropods. Include the disease name, specific organism (genus species), vector that is the intermediate between hosts, and disease progress in humans.

Answers

Answer:

List three protozoan infections of the circulatory system that are transmitted by arthropods:

Malaria

Toxoplasmosis

Chagas disease (American trypanosomiasis)

Explanation:

Malaria is a prozotoan infection, that can be produced by several protozoan, in the genus Plasmodium. This Plasmodium infect red blood cells and the vector that trasmites it is the Anopheles mosquitoe. In this disease, red blood cells can have severe damage in different organs because of the hemolysis.

Toxoplasmosis is a disease caused by the protozoan Toxoplasma gondii. The main vector of them are domestic cats. When cats are infected, T. gondii oocysts is found in their feces and it can spread to humans. This disease is generally asymptomatic, except when the patient has AIDS or in pregnancy, which can be very dangerous and fatal.

Chagas disease, or American trypanosomiasis is caused by protozoan Trypanosoma cruzi and is generally transmitted to people thanks to the feces of triatomine bugs. Some of the acute symptoms include fever, vomiting, headache, and in severe cases people can develop a chronic disease. In the worst cases it can produce flaccid cardiomegaly (enlargement of the heart) leading to heart failure and death.

Which type of muscle contracts without nervous stimulation?

Answers

Answer:

the answer is the cardiac muscle

The end result of a chemical reaction is always:
A. the formation of new kinds of elements.
B. the production of water molecules.
c. a substance that was not ope of the reactants.
ak
s
D. a molecule that does not have an electric charge.

Answers

Answer:

The formation of new kinds of elements.

When a chemical reaction is finished, a new type of element is made. because the chemicals couldn't react to eachother without making something new.

Pectoralis Minor External Obliques Gluteus maximus Hamstrings Which are axial muscles or appendicular muscles?

Answers

Answer:

they are axial muscles

For the cells of the body to function properly, blood pH must be maintained between 7.35 and 7.45, a very narrow range. Buffer systems, the respiratory system, and the kidneys work together to help maintain acid-base balance.

a. True
b. False

Answers

Answer:

ddddddddddddddddddddddddddddddddddddddddd

Explanation:

Which of the following is a testable scientific question

Answers

Where are the questions so i can answer them?

Answer: Just took the test: Its "How does the age of city affect the number of trees in the city? "

What are some of the main characteristics of skeletal muscle cells that make them distinct from the other two types of muscle cells Why are these characteristics important for understanding the function of skeletal muscle?

Answers

Answer:

They are voluntary , require force and fast.

Explanation:

Main characteristics of skeletal muscle cells are given below:

1) these muscles are voluntary which means it can be controlled by the human.

2) skeletal muscles requires force for its movement.

3) movement of skeletal muscle is fast.

Due to its structure, skeletal muscle provide support to the body and the body is able to move from one place to another. It also provide protection to the delicate organs of the body. It is also used as a storage of minerals and fats.

Draw a cross section of a peach, and label the structures. What parts of the flower give rise to these structures

Answers

Answer:

exocarpo  is synonymous with exocarp

endocarpo is synonymous with endocarp

mesocarpo is synonymous with mesocarp

semilla is synonymous with seed

Explanation:

In phase one, the plant's ovary begins to grow abruptly, this is where the embryo and endosperm affect their size, but if this ovarian growth is interrupted by an external factor, it determines the size of the future.

Then, the embryo and the endosperm could grow and undergo a process called sclerification, which is the hardening of the interior of the fruit, that is to say, the future formation of the seed or vulgarly called "pit". .

After this, the mesocarp, which is what is called the fleshy body or subglobose edible drupe, begins to grow.

The growth of the mesocarp is what gives the volume, weight and body to the fruit, so that after maturation it exceeds the strength of the branch and it falls from the tree.

Which sentence about protist is accurate

Answers

Answer:

there are no sentences

Explanation:

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