Choose a strategy to clone genes known only by a mutant phenotype in organisms with highly efficient transformation procedures and relatively small genomes.
a. cloning by complementation
b. direct sequencing of the mutant genome
c. PCR of the gene and transformation of an organism
d. cloning using a specific cloning vector

Answers

Answer 1

Answer:

Option a (cloning by complementation) `would be the correct choice.

Explanation:

In species containing extremely efficient transforming techniques as well as comparatively small genomes, cloning by rearrangement testing is performed to clone gene products mostly by a mutated gene as the rearrangement test was being used to detect mutations.This strategy for cloning through mechanism begins with identifying another yeast mutant that affects something like a plasmid representing a cloned genetic material.

The other options in question aren't relevant to the particular circumstance. Therefore the answer to the above seems to be the right one.


Related Questions

What term is used to describe a retrograde flow of urine from the urinary bladder into the ureters that is the cause of recurrent urinary tract infections (UTIs)?

Answers

Answer:

Vesicoureteral reflux (VUR)

Explanation:

Answer:

The condition is known as Vesicoureteral reflux (VUR)

Explanation:

Vesicoureteral reflux (VUR) is a pathological condition caused by Urinary Tract Infections (UTIs) where urine flows back from the bladder into one or both ureters, and sometimes back into the kidneys. The condition is commonly seen in young children under 2 years of age, and infants.

Most children with primary VUR were born with an abnormal ureter, which is mainly seen as a defective valve between the kidneys and the ureters that does not close well, hence resulting in refluxes. Secondary VUR develops due to some pathological conditions affecting the urinary tract, like blockage of the urethra, causing reflux of urine. Defective bladder nerves can also lead to VUR.

Please answer ASAP, the assignments in due really soon :) 30 Points!!! The image below shows the lac operon, which breaks down lactose in bacteria. Which statement correctly describes the expression of this gene?

Answers

Answer:

B. Gene expression is influenced by high lactose levels in the environment.

Explanation:

The lactose operon (lac operon) functions for the metabolism and transport of lactose in several bacteria.

Gene expression is influenced by high lactose levels in the environment as genes are expressed in the presence of high level lactose and absence of glucose. High level lactose carry an activator protein called catabolite activator protein (CAP), which let the RNA polymerase do transcription of the operon.

Hence, the correct option is "B".

Answer:

C

Explanation:

^he said it was right^

have an amazing day! :)

What do enzymes do? Please answer real quick

Answers

Answer:

Catalyze biochemical reactions.

Explanation:

Hello,

In this case, it is widely known that enzymes are able to take substrates or reactants to their active sites, in order to catalyze a raft of biochemical reactions by which products that are not usually formed under typical conditions are formed, and then allow them to dissociate from the surface of the enzyme. Such combination formed by an enzyme and its substrates is called the enzyme–substrate complex which is further taken to the isolated product.

Regards.

Is minerals renewable or non renewable?

Answers

Answer:

Minerals are non renewable if it is fossil......

Answer: Minerals are nonrenewable resources.

Explanation:

A nonrenewable resource is a resource that can not be replenished during a human lifespan. Minerals, metal ores, and fossil fuels are examples of nonrenewable resources.

define factors affecting enzyme action:temperature

Answers

Answer:

I am secondary level student and this doesn't belongs to my coure till yet however..i have written this through google.....you may search there as well..

Explanation:

Enzyme activity occurs within a narrow range of temperatures compared to ordinary chemical reactions. As you have seen, each enzyme has a certain temperature at which it is more active. This point is called the optimal temperature, which ranges between 37 to 40C°...

Temperature. As temperature increases to the optimum , the kinetic energy of the enzyme and substrate increases, causing more collisions between the enzyme and substrate.

Which statement best describes a hypothesis?

Answers

Answer:

A hypothesis is an idea or explanation you can test through study and experimentation.

Explanation:

What type of muscle is found in the circulatory system?

Answers

Answer:

The heart is the pump and the vessels are the delivery and return system for the blood. Every tissue in the body, directly or indirectly, receives oxygen and nutrients from blood supplied by the cardiovascular system. The heart is a muscle. The muscle is a modified form of skeletal muscle called cardiac muscle.

Explanation:

i hope itt help you

Answer:

blood muscles are found

5. According to the video, what is the goal of the tree of life?
a. To summarize a fossil record
b. To describe how natural selection works
To be a library of all life that has ever lived
d. To explain how all species are related to each other

Answers

To be a library of all life that has ever lived

Which organelle is labeled I? cell membrane ribosome endoplasmic reticulum nucleus

Answers

Answer:

"nucleus" seems to be the correct answer.

Explanation:

The nucleus seems to be a significant case wrapped through a double membrane that distinguishes these from the cytoplasm, the nuclear shell. The membranes combine throughout a few areas, allowing the interchange of substances between some of the cytoplasm as well as the nucleus, steps given designated "nuclear pores".The nuclear shell is consistent with either the concentrated endoplasmic reticulum, thereby bonding with either the nucleus.

Answer:

Nucleus

Explanation:

What is a CAFO? Spell out what the acronym stands for. Briefly describe what the animals experience there. How does the way they are treated also affect us?

Answers

Answer:

Concentrated Animal Feeding Operation

Explanation:

CAFO stands for Concentrated Animal Feeding Operation.

CAFO helps in the efficient care of the animals. They are provided with good food sources and also help provide a standard shelter for their optimal production.

It affects us as there is a high probability on the presence of cheap and high quality livestock products such as egg, meat etc due to the efficiency in animal care carried out.

A 23 year old female presents with bilateral loss of central vision. You obtain a detailed family history and produced the associated pedigree. Which of the following transmission patterns is most consistent with this patient's family history?

a. Autosomal dominant
b. Autosomal recessive
c. X-linked dominant
d. X-linked recessive
e. Mitochondrial

Answers

Answer:

e. Mitochondrial

Explanation:

This is a case of mitochondrial heredity so correct answer is e. Mitochondrial In this case, the mother transfers her mitochondria to her children. So the child is affected. The male does not pass this trait on to his children. We can see from the lineage that the mother has defective mitochondria so all their children will have defective mitochondria. One of his sons has mitochondrial, but is unable to pass it on to the next generation.

Describe how protein functional groups can act as acid and base catalysts. How is it possible for a single amino acid side chain to function as both?

Answers

Answer:

By changing the transition state and pH of the medium

Explanation:

Enzymes are proteins that bind to their corresponding substrates at their active sites in order to form complexes that catalyze specific chemical reactions. A key feature of this process is the fact that both acid and base catalytic activities may be combined in the same reaction. The acceleration in the velocity of the enzymatic reaction due to the addition of acids and bases is known as acid–base catalysis, where the acid or base is not consumed during the reaction. For example, enzymes can partially transfer a proton to the medium, thus changing the transition state of the chemical reaction and also decreasing its pH.

In prokaryotic cells, regulator proteins bind to a section of DNA called alan (1 point)
O repressor
o promoter
O chromatin.
o operon.

Answers

Answer:

promoter

Explanation:

Answer: Operon

Explanation:

Alexei Romanov suffered from hemophilia, a condition that (1 point)
causes the body to produce too many blood cells due to the body's inability to form fibrin proteins.
o prevents blood from clotting normally due to the body's inability to form fibrin proteins.
O causes the body to produce abnormally shaped red blood cells due to the body's inability to form fibrin proteins.
o prevents the body from absorbing vitamin B12 due to the body's inability to form fibrin proteins.

Answers

Answer:

prevents blood from clotting normally due to the body's inability to form fibrin proteins.

Explanation:

Haemophilia is a genetic disorder which could be inherited and it is characterized by constant bleeding and loss of blood when injuries occur. This is as a result of the inability of the body to form fibril proteins. The fibril proteins are responsible for the production of platelets . The platelets are responsible for the clotting of blood.

This validates haemophilia being a disorder which prevent blood from clotting normally due to the body's inability to form fibrin proteins.

For those who need more answers

1. Adenine

2. Prevents blood from clotting normally due to the bodys inability to form fibrin proteins

3. TCA

4. DNA has the sugars deoxyribose, while RNA has the sugar ribose

5. tRNA carries amino acids to the ribosome and helps form proteins

what is magnetic induction ​

Answers

Answer:

The process of gaining or obtaining temporary magnetism in magnetic material due to the effect of magnet is called magnetic induction.

Answer:

Hey buddy here is your answer:-Magnetic Induction is the process by which an object or material is magnitized by an external field...

Drag each tile to the correct box. Place the items in order from lowest to highest degree of internal organization. Please answer 1st at the top and last at the botttom

Answers

Answer:

Lowest to highest degree of internal organization form the given items are:

1) Digestive system: Digestive system is a type of organ system, which is consist of several digestive organs such as mouth, stomach, pancrease and intestine.

2) Stomach: Stomach is a type of organn which is a functional group of tissues.

3) Stomach lining tissues: These are functional group of cells.

4) Epithelial stomach cell: cell is a basic unit of all life.

Hence, the correct order form loowest to highest degree of internal organization is:

Digestive system => Stomach => Stomach lining tissues => Epithelial stomach cell

Each of two parents has the genotype brown/red​, which consists of the pair of alleles that determine hair color​, and each parent contributes one of those alleles to a child. Assume that if the child has at least one brown ​allele, that color will dominate and the​ child's hair color will be brown. a. List the different possible outcomes. Assume that these outcomes are equally likely. b. What is the probability that a child of these parents will have the red/red ​genotype? c. What is the probability that the child will have brown hair color​? a. List the possible outcomes.

Answers

Answer:

a. In image attached.

b. 25% or a 1 in 4 chance.

c. 75% or a 3 in 4 chance.

Explanation:

B = Dominant (brown gene)

r = Recessive (red gene)

If two parents both have Br genes, they will both have brown hair.

The Punnet square will look like the image attached.

When a liquid becomes a gas, _____

The molecules move closer together
The molecules move farther apart
The molecules are unchanged
There are no molecules

Answers

Answer:

The molecules move farther apart.

The ion composition of perilymph is like that of interstitial fluid; high sodium and low potassium levels. If someone ruptured the vestibular membrane, the membrane between the vestibular duct (Scala vestibuli) and the cochlear duct (Scala media), what would be the effect of such a rupture on the concentration of potassium in the endolymph in the cochlear duct

Answers

Answer:

The concentration of potassium will decrease

Explanation:

The cochlear ducts are composed of two distict types of fluids: 1-perilymph, rich in sodium (140 mM) and low in potassium (5 mM), and 2- endolymph, rich in potassium (1 mM) and low in sodium (150 mM). The perilymph fluid has a similar composition to the rest of the extracellular fluids, while endolymph present a unique ion composition that is not found in other fluids of the body. It is believed that the cochlea can regulate potassium flow without using energy (i.e.,  without use ATP), since potassium enters into the cells through passive pathways, while this ion ion leaves the cells by an active mechanism.

Challenge Question! If a pregnant woman had a karyotype prepared for her baby before birth, would it be more useful in predicting the risk of Down Syndrome or Tay Sachs disease?

Answers

Answer:

Yes

Explanation:

Karyotype involves the process of viewing a persons chromosomes through Sophisticated equipments.

Down Syndrome or Tay Sachs disease are genetic diseases associated with chromosomal defects.

Having a karyotype prepared for her baby before birth, would be more useful in predicting the risk of Down Syndrome or Tay Sachs disease.

What energy transformations are going on within the skateboarder’s body during this process?

Answers

Answer:

When they slide down, they lose this potential energy, which turns into kinetic energy and thermal energy. Thermal Energy - This energy is related to the temperature of a body or a system. When friction is involved in a system, part of the kinetic energy is transformed into thermal energy.

g You can find the results of the seedling experiment hereLinks to an external site.. Carry out the hypothesis test that you have identified in the previous problem; which is closest to the p-value that you obtain?Group of answer choices0.040.080.090.61

Answers

Answer:

0.080

Explanation:

The seedling experiment is the mobilization of reserves during the seed experiment. Development of embryo exist in the seedling. When the test is carried out the p-value obtained is near to 0.781 which is approximately 0.080. The calculation is 10 * 0.07 / 8.75 after solving the equation which we get is 0.080.

Describe at least 2 benefits and 2 drawbacks there might be for animal cells (including humans) to make their own food through photosynthesis.

Answers

Answer:

Explanation:

Benefits

Animals will not depend on plant source again for their food but have it produced directly by themselves because photosynthesis will allow animal produce their own food

Animal will get a direct source of energy for their activities. Energy is derived from food consumed after the food has been broken down in the body system of animal. Animal photosynthesis will give animals access to direct source of energy as the product their food.

Demerit

Animal lacks chlorophyll the green. Pigment in plant that light hit on absorption that will enable them to photosynthesis.

Animal lacks ways or mechanism of regulating Carbondioxide in take as in the case of C4 plant and crassulacean metabolic pathway (CAM).

Animals such as human will not have access to varieties of food but stick to photosynthate produced by them.

PLEASE ANSWER QUICK!The Greek roots of the word prokaryote mean “before nucleus.” Describe the way that DNA is organized in prokaryotic cells without the help of a nucleus. How does this approach differ from the way that eukaryotic cells organizes their DNA

Answers

Answer:

Prokaryotic cells' DNA are located in the cytoplasm of the cell rather than in the nucleus, like in eukaryotic cells. DNA aids in protein synthesis and determines functions of the cell in cells, regardless of being within the membrane of a nucleus or not.

-----

I hope this helps a little.

The word prokaryote in Greek means before kernel (nucleus). Unlike the eukaryotic cells, the nuclear material is located in the cytoplasm of the cell in a nucleoid.

What are the characteristics of prokaryotic cells?

The prokaryotic cells are the primitive karyons that are defined by the lack of the true nucleus and organelles. Unlike the eukaryotes, the organelles lack the membrane that covers them but has a tough cell wall.

The prokaryotes include archaea and bacteria which are unicellular and microscopic organisms that are simple and have their genetic material organized into nucleoids in the center of the cell. They have the ability to live in harsh conditions.

Therefore, the eukaryotes and prokaryotes differ in the arrangement of the genetic material.

Learn more about prokaryotes, here:

https://brainly.com/question/18348786

#SPJ2

Which are the 3 major areas of society affected by science?

Answers

Answer:

Explanation:

the three major areas of society affected by science are economic democratic and cultural

mark me brainlist if my answer is right

Genes, Chromosomes, and DNA Quick Check
If one half of a DNA molecule has 8 thymine bases, what else is also true about the DNA molecule? ( point)
There will be 16 total bases.
There will be 32 total bases.
There will be 8 adenine bases.
There will be 8 cytosine bases.

Answers

Answer:

There will be 8 adenine bases.

Explanation:

There are total of 4 nitrogenous bases present in a DNA that includes Adenine, Guanine, Thymine, and Cytosine. These nitrogenous bases form complementary base pair in which, adenine form two hydrogen bonds with thymine and cytosine form three hydrogen bond with guanine.

So, if there are 8 thymine bases in a DNA (irrespective of half or full) then there will be 8 adenine bases, forming two hydrogen bond with each other.

Hence, the correct answer is "There will be 8 adenine bases."

The photoelectric effect describes when light shines on a piece of metal, and the metal releases electrons. Which model of light
behavior best helps explain this effect?

Particle model

Wave model

Electromagnetic model

Frequency model

Answers

Answer:

The answer is PARTICLE MODEL The person above ^^^ got the answer wrong, the correct answer really is Particle Model...

Thanks...

Answer:

Particle model

Explanation:

I am taking the test rnn

Flvs

Describe the intermolecular interaction of the surface carboxylate groups of a micelle and the surrounding water.

Answers

Answer:

Hydrophilic portions are outside and hydrophobic regions are inside

Explanation:

A micelle is an assembly of amphiphilic molecules formed in a liquid solution, generally an aqueous solution. Micelles are formed due to the packing (aggregation-like) behavior in which single-tail lipids are organized in a bilayer. A typical micelle in aqueous solution forms a spheric assembly where carboxylate ions are organized in a mode that hydrophilic head regions enter in contact with the surrounding water, while hydrophobic (lipophilic) portions are disposed inside the micelle.

Butterflies can produce hundreds of offspring per cross. In a certain variety of butterflies, a maternally-imprintable gene is responsible for wing phenotype. Two possible alleles for this gene are w for normal wings and wc for crinkled wings. Which of the following pieces of information would be helpful in predicting the wing phenotype of a male offspring from a cross?

a. look at the father's phenotype
b. know the mother's genotype
c. look at the mother's phenotype
d. know the father's genotype

Answers

Answer: d. know the father's genotype

Explanation: Butterflies can produce hundreds of offspring per cross. In a certain variety of butterflies, a maternally-imprintable gene is responsible for wing phenotype.

The option that would be helpful in predicting the wing phenotype of a male offspring from such a cross would be to look at the father's phenotype.

Imprinted genes can simply be defined as genes that are expressed in parent-of-origin specific manner and result from a chemical modification of DNAs.  Such genes are differentially expressed depending on which of the two parents they are inherited from. Hence:

maternally-imprintable genes are only expressed when they are inherited from the father. paternally-imprintable genes are only expressed when inherited from the mother.

Phenotype refers to the physical appearance or expression of a gene. Therefore, if a gene is maternally imprintable, looking at the phenotype of the father would be helpful in predicting the phenotype of the male child in case a cross happens.

Applying the same principle to the butterfly, it will, therefore, be helpful to look at the father's phenotype in order to predict the wing phenotype of a male offspring from a cross.

The correct option is A.

More about imprinted genes can be found here: https://brainly.com/question/7207412

during what stage of swallowing is there a risk of food entering respiratory pathways

Answers

Gulping... I think this is the stage you are looking for

Hope this helped ♥︎
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