A technique that can be used to separate proteins based primarily on the presence of non-polar residues on their surface is called A) ion-exchange chromatography. B) gel filtration chromatography. C) affinity chromatography. D) gel electrophoresis. E) hydrophobic interaction chromatography.

Answers

Answer 1

Answer:

E) hydrophobic interaction chromatography.

Explanation:

Hydrophobic interaction chromatography (HIC) is a methodology used to separate protein molecules depending on their hydrophobicity. HIC enables the separation and purification of proteins by using a reversible interaction between the target protein and the hydrophobic ligand of a HIC resin such as, for example, methacrylate-based beads. This interaction is influenced by the running buffer (high salt concentrations favor hydrophobic interactions). HIC enables the separation of proteins under low denaturation conditions, thereby preserving their biological functions.


Related Questions

¿Cuál de los siguientes niveles es el sucesor del nivel tejido?
A.
Órgano.
B.
Sistema.
C.
Organismo.
D.
Célula.

Answers

answer: D
bcuz i said so and this

C. Órgano

Porque Célula→Tejido→Órgano→Sistema→Organismo

Which of the following statements is MOST accurate?
a. Most people who engage in gambling activities become addicted to it
and end up taking bankruptcy because they cannot pay their bills.
b. People at lower income levels lower use a larger percentage of their
income to buy lottery and scratch tickets than people at higher levels of
income.
c. Gambling is a moral issue, with little or no economic or financial impact
on individuals or society.
d. Casinos only have a positive impact on communities because they create
jobs and generate wealth.

Answers

Answer:

C

Explanation:

Chemosynthesis _______. Question 11 options: uses energy derived from hydrocarbons and hydrogen sulfide in place of solar energy produces carbohydrates and sugars occurs around hydrothermal vents near the oceanic ridge system Both a and c are correct. All of the above are correct.

Answers

Answer:

The answer is all of the above are correct.

Explanation:

Chemosynthesis occurs in bacteria and other organisms and involves the use of energy released by inorganic chemical reactions to produce food. All chemosynthetic organisms use energy released by chemical reactions to make a sugar, but different species use different pathways. For example, at hydrothermal vents, bacteria oxidize hydrogen sulfide and add carbon dioxide and oxygen to produce sugar, sulfur, and water:  

CO2 + 4H2S + O2 ⇒ CH20 + 4S + 3H2O.

Chemosynthesis is the process by which food (glucose) is made by bacteria using chemicals as the energy source, rather than sunlight. Chemosynthesis occurs around hydrothermal vents and methane seeps in the deep sea where sunlight is absent. During chemosynthesis, bacteria living on the seafloor or within animals use energy stored in the chemical bonds of hydrogen sulfide and methane to make glucose from water and carbon dioxide (dissolved in seawater). Pure sulfur and sulfur compounds are produced as by-products.

What factor is described as sexual
selection?
A, non-random mating
B. migration
C. small populations
D. natural selection

Answers

Answer:

The correct answer is - D. natural selection.

Explanation:

Sexual selection takes place due to competition between individuals of the intraspecies same-sex and of mate choice which driving the evolution of reproductive traits.

Sexual selection is a mode of natural selection as it leads to the evolution of specific traits. Other examples or options are not related to sexual selection directly and are not affected by sexual selection.

Write down the functions of
1.microtubules
2.tripletsof microtubules

Answers

Answer:

Microtubules are filamentous intracellular structures that are responsible for various kinds of movements in all eukaryotic cells. Microtubules are involved in nucleic and cell division, organization of intracellular structure, and intracellular transport, as well as ciliary and flagellar motility

Centrioles are cylindrical structures that are made up of protein tubes called microtubules. Specifically, nine groups of three microtubules, known as triplet microtubules, are linked together to make the walls of the cylinder.

Hope it helps :)

Answer:

Microtubules have several functions. For example, they provide the rigid, organized components of the cytoskeleton that give shape to many cells, and they are major components of cilia and flagella (cellular locomotory projections). They participate in the formation of the spindle during cell division (mitosis).

triplet microtubules are linked together to make the walls of the cylinder.

Explanation:

the drug which is used to reduce pain is ..,....

i) opium ii) bhang iii) hashish iv) marijauna​

Answers

Answer:

hashish

Explanation:

opinum

Explanation:

because it is used to make pain nubbing/pain killer

which describe the complex carbohydrate cellulose

Answers

Answer:

Cellulose is a polysaccharide consisting of a linear chain of several hundred to many thousands of linked glucose units. Cellulose is an important structural component of the cell walls of plants and many algae.

Explanation:

Explain how homologous and the fossil records support evolution.
Thanks so much!!!

Answers

Answer:

Los fósiles, una prueba de la evolucion. El estudio de los fósiles nos da una idea muy directa de los cambios que sufrieron las especies al transformarse unas en otras; existen muchas series de fósiles de plantas y animales que nos permiten reconstruir cómo se fueron adaptando a las cambiantes

Explanation:

Answer:

homologous: have identical position, value, or structure

Fossils: In the oldest rocks area unit fossils of the best organisms and within the newest rocks area unit fossils of additional advanced organisms.

 

Explanation:

The evolution theory is supported by multiple sorts of evidence: homologous structures show common ancestry, whereas similar selective pressure will manufacture similar variations. analogous structures (beneficial features).

In the oldest rocks area unit fossils of the best organisms and within the newest rocks area unit fossils of additional advanced organisms. This supports Darwin's organic process theory, whereby straightforward life forms {progressively|increasingly|more and additional} evolved into more advanced. Proof of adolescence forms comes from fossils.

Can someone please help me!

Answers

Answer:

thats the 2 number point okay,plasma contains platelets ,ok

Explain the reciprocal relationship between human society and limiting factors

Answers

Answer:

humans try to modify environment factors to suit their needs. Limiting factors can be controlled in a specific area however, we can't control larger area. Like water or energy.

How do I make a Punnet Square without knowing if traits are Dominant or Recessive? I cannot refer to the parents of the current generation, so I have to rely on that generation to create a theoretical next generation.

Answers

If you are referring to a previous generation, that would probably mean that the traits they have are the dominant ones

Explanation:

if you know all the traits possible, then the ones the parents have of a previous generation are most likely the dominant ones, from there, you can hopefully figure out another generation. could you attach a picture if this didn't help? thanks

Why are photsynthetic aquatic and marine protists important to ecosystems
(remember to include algaes)?

Answers

Protists and their products are essential, indirectly and directly in photosynthesis because they help organisms through their survival and ranging from bacteria to mammals. Protists feed tons of the worlds aquatic animals and species and they do not only create food but they also provide sources for sea dwelling organisms

Which is the difference between an antibiotic and an antiviral?

Answers

Answer:

There are no options in this question, the options are as follows:

A. Antibiotics build up immunity, and antivirals increase white blood cells.

B. Antibiotics treat the symptoms, and antivirals destroy the causing organism.

C. Antibiotics are in liquid form, and antivirals are in mist form.

D. Antibiotics treat patients who have Staphylococcus, and antivirals treat patients who have the flu.

The answer is D

Explanation:

Specific drugs are used to curb infection or diseases caused by specific pathogens. An infection can be caused by either bacteria, virus or fungi. Considering their different mechanism of infection, viral infections are treated with the use of ANTIVIRAL DRUGS while bacterial infections are treated with the use of ANTIBIOTICS.

According to this question, staphylococcus is a bacterial genus and hence, patients with staphylococcus can be treated with antibiotics while flu is a viral disease, hence can only be treated with antivirals.

Answer:

A. Antibiotics build up immunity, and antivirals increase white blood cells.

Explanation:

edg

if the velocity of a vehicle starting from rest reaches 5m/2s in 2seconds, is the velocity of the vehicle uniform ?explain.​

Answers

Explanation:

Avg speed × total times = total distance

20×25=500

Since acceleration in the 1st interval is same as deceleration in the 3rd interval and deceleration is from the same velocity achieved after acceleration, t

1

=t

3

s

1

=

2

1

×5t

1

2

s

2

=(5t

1

)t

2

s

3

=(5t

1

)×t

3

2

1

×5×t

1

2

=(5t

1

)×t

1

2

1

×5×t

1

2

s

1

+s

2

+s

3

=5t

1

t

2

+5t

1

2

⇒5t

1

2

+5t

1

t

2

=500−−−−1

2t

1

+t

2

=25−−−−2

Solving (1 ) and (2) ⇒t

1

=5,t

2

=15sec

A cell membrane is very specific about what it allows to cross.how does this help the cell?

Answers

Answer:

The cell membrane is selectively permeable . It lets some substances pass through rapidly and some substances pass through more slowly, but prevents other substances passing through it at all. Some small molecules such as water, oxygen and carbon dioxide can pass directly through the phospholipids in the cell membrane

List three of water’s unique properties.

Answers

Explanation:

1.water is called universal solvent it can dissolve almost every solute on it

2.water can show anamolus expansion which mean it density will me most on 4 degree celsius

3.it is neutral substance

universal solvent, water cane expand, neutral substance

The pun nett square is for a test cross of two mice That were purebred

Answers

i don’t know this answer my b

Hitchhikers thumb (H) is dominant to no hitchhikers thumb (h). A woman who does not have hitchhiker some marries a man who is Carretera genus for hitchhikers thumb. What is the probable genotypic of their children?

Answers

Complete question:

3. Hitchhiker’s thumb (H) is dominant to no hitchhiker’s thumb (h). A woman who does not have hitchhiker’s thumb marries a man who is heterozygous for hitchhiker’s thumb. What is the probable genotypic ratio of their children? What is the probable genotypic of their children?

Answer and Explanation:

Due to technical problems, you will find the complete answer and explanation in the attached files

The statements on the left are incorrect
Write the correct statement.
Incorrect statements
1. Breathing produces carbon
dioxide from glucose
2. Only animals carry out
respiration. If someone can help with this it’d be greatly appreciated

Answers

Answer:

Carbon dioxide creates oxygen so we can breathe and seperates it from glucose

All species and humans breathe fish have gills to breathe underwater so not just animals have respiration.

Explanation:

How do the frequencies of the waves compare?

Wave A has a higher frequency because it has a larger amplitude.
Wave A has a lower frequency because it has a longer wavelength.
The waves have the same frequency because they have the same wavelength.
The waves have the same frequency because they have the same amplitude.

Answers

Answer:

The waves have the same frequency because they have the same amplitude.

. Most of the matter in the universe: A. Exists within stars B. Is made when planets are formed C. Is unknown, called dark matter and energy D. Is blue-shifted

Answers

Answer:

B

Explanation:

is made when planet are formed

can someone help me?

Answers

Answer:

I believe it's A, commencualism.

Explanation:

Because it means that one type of animal pairs itself with another to provide a service or perk, while the other maybe offers protection or something similar, improving the species.

I hope it's correct tho, sorry if it's not. ♥

Which of the following newborns has the lowest chance of having health problems?
A. Rosalind- 6 lbs
B. Charles- 11 lbs
C. Nikola- 7.5 lbs
D. Marie- 5 lbs

Answers

Answer:

C Nikola

Explanation:

7.5 ibs is the most average and ideal weight for newborns.

Rosalind is the newborn who the lowest chance of having health problems. Thus, the correct option is A.

What are health problems?

Health problems may be defined as a situation of disease or medical ailment or an environmental condition that poses the threat of disease or medical ailment practically.

Rosalind weighs 6lbs which is equal to 2.72 kilograms. The most ideal and normal weight of newborns should be ranging in between 2.5 to 3 kilograms.

Therefore, Rosalind is the newborn who the lowest chance of having health problems. Thus, the correct option is A.

To learn more about Health problems, refer to the link:

https://brainly.com/question/3797215

#SPJ5

write at least three difference between arteryand vein with diagram​

Answers

bonjour,

1. Arteries carry blood from the heart to the rest of the body, whereas veins carry blood from the rest of the body back to the heart.

2. Almost all arteries carry oxygenated blood and almost all veins carry deoxygenated blood.

3. Arteries have a thick elastic muscle layer, whereas the muscle layer for veins is much thinner.

Answer:

Difference Between Artery and Vein Artery 1. Vessels which carry blood from the heart to various body parts  2. Arteries carry oxygenated blood from the heart except pulmonary artery  3. Arteries have thick elastic muscular walls  4. Valves are absent  5. Blood flows under high pressure  Vein  1. Vessels which carry blood from the various body parts to the heart  2. Veins carry deoxygenated blood from the various body parts except pulmonary vein  3. Veins have thin non elastic walls  4. Valves are present to prevent the backward flow of blood  5. Blood flows under low pressure

In order for natural selection to occur, organisms must (select all that apply) Check All That Apply have variation that can be passed from one generation to the next. have variation that can be passed from one generation to the next. have differential reproductive success. have differential reproductive success. survive longer than another organism. survive longer than another organism. compete for resources. compete for resources. have adaptations to their environment. have adaptations to their environment.

Answers

Answer:

have variation that can be passed from one generation to the nextsurvive longer than another organismhave adaptations to their environment. have differential reproductive success.

Explanation:

Naturla selection is the survival and reproduction of individuals due to different phenotypes. The naturla selection represents a mechanism of evolution and changes in traits of species.

In order for natural selection to occur, organisms must :

have a variation that can be passed from one generation to the next.have differential reproductive success.

Natural Selection is a mechanism of evolution that occurs when there is a heritable variation for a trait and individuals with one version of the trait have greater reproductive success than do individuals with a different version of the trait.

In order for natural selection to occur, three conditions must be met:

There must be variation for the particular trait within a population.The variation must be inheritableIndividuals with one version of the trait must produce more offspring than those with a different version of the trait.

Thus,

In order for natural selection to occur, organisms must :

have a variation that can be passed from one generation to the next.have differential reproductive success.

Learn more:

https://brainly.com/question/15577096

Complete the table by filling in the missing information. Use these choices:

frameshift
substitution


7. UGU-CCG-GAA-CGA

UGC-CGG-GAA-CGA

8. GAA-CGU-AGC-GGU

GAU-CGU-AGC-GGU

9. UGU-UUC-CCU-UAA

UGU-UCC-CUU-AA

Answers

Answer:

7. Substitution mutation

8. Substitution mutation

9. Frameshift mutation

Explanation:

A substitution mutation is any kind of mutation that involves replacement of one or more nucleotide base by another in a sequence.

A frameshift mutation, on the other hand, is a mutation that changes the reading frame of the sequence. Two types of mutations cause frameshift viz: insertion and deletion mutation.

In the following sequences, mutation has occured as follows:

7. UGU-CCG-GAA-CGA to UGC-CGG-GAA-CGA - Substitution mutation has occured because nucleotides C and G has replaced C and U in the first and second codons respectively.

8. GAA-CGU-AGC-GGU to GAU-CGU-AGC-GGU- A substitution mutation because nucleotide U has replaced A in the first codon.

9. UGU-UUC-CCU-UAA to UGU-UCC-CUU-AA - A frameshift mutation because nucleotide U has been removed from the second codon, hence, causing a change in the reading frame.

what is science?????​

Answers

Answer:

Science is the study of nature around us.

Answer:

As scientist discovered that Science is the method of learning everything about the natural world.

Which of the following best describes the difference between a cation and an anion?

Answers

Answer:

An anion is a negatively charged ion that has gained more electrons than protons and added them to it's energy levels. A cation is a positively charged ion that has lost electrons from it's energy levels. It also has more protons than neutrons.

Explanation:

The diagram below shows a light ray striking Medium A and Medium B at the same angle. (4 points)

A rectangle labeled Medium A is drawn on the left. A ray of light travels through air and strikes the surface of medium A making an angle of 50 degrees with the vertical. The light ray bends towards the vertical as it travels through Medium A and makes an angle of about 15 degrees with the vertical. A rectangle labeled Medium B is drawn on the right. A ray of light travels through air and strikes the surface of medium B making an angle of 50 degrees with the vertical. The light ray bends towards the vertical as it travels through Medium B and makes an angle of about 25 degrees with the vertical.

Which statement is correct?
The speed of the light ray is the same in air and Medium A.

The speed of the light ray is the same in air and Medium B.

Medium A and Medium B have different densities because light refracts at different angles.

Medium A and Medium B have the same density because the light ray strikes both at the same angle.

Answers

Answer:

A the speed of the light ray is the same in air and medium

Which of the following is a heterotroph?
*cyanobacteria
*gopher
*fir tree
*dandelion

Answers

Correct Answer is gopher
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